Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IG196_RS24150 Genome accession   NZ_CP062121
Coordinates   5193026..5194255 (+) Length   409 a.a.
NCBI ID   WP_192193333.1    Uniprot ID   A0A7L8SD51
Organism   Variovorax sp. 38R     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5188026..5199255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG196_RS24120 (IG196_24120) cgtA 5188117..5189193 (-) 1077 WP_192193331.1 Obg family GTPase CgtA -
  IG196_RS24125 (IG196_24125) rpmA 5189267..5189527 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  IG196_RS24130 (IG196_24130) rplU 5189540..5189851 (-) 312 WP_042578903.1 50S ribosomal protein L21 -
  IG196_RS24135 (IG196_24135) - 5190034..5191026 (+) 993 WP_192193332.1 polyprenyl synthetase family protein -
  IG196_RS24145 (IG196_24145) pilB 5191268..5193001 (+) 1734 WP_101488972.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IG196_RS24150 (IG196_24150) pilC 5193026..5194255 (+) 1230 WP_192193333.1 type II secretion system F family protein Machinery gene
  IG196_RS24155 (IG196_24155) - 5194255..5195220 (+) 966 WP_192193334.1 prepilin peptidase -
  IG196_RS24160 (IG196_24160) coaE 5195234..5195845 (+) 612 WP_192193335.1 dephospho-CoA kinase -
  IG196_RS24165 (IG196_24165) - 5195922..5197415 (+) 1494 WP_192193336.1 MBOAT family O-acyltransferase -
  IG196_RS24170 (IG196_24170) - 5197422..5198450 (+) 1029 WP_192193337.1 hypothetical protein -
  IG196_RS24175 (IG196_24175) zapD 5198487..5199242 (+) 756 WP_101488967.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44743.48 Da        Isoelectric Point: 9.5343

>NTDB_id=486143 IG196_RS24150 WP_192193333.1 5193026..5194255(+) (pilC) [Variovorax sp. 38R]
MATVASSRSQVTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKSIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANPSVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFIVVAIIMIFVIPAFKEVFTSFGADLPAPTLIVMAISEFFVSYWWLIFGVLGGGI
YFFLQAWKRNERVQKVMDRLLLRLPIFGTLIEKSCIARWTRTLATMFAAGVPLVEALDSVGGASGNSVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=486143 IG196_RS24150 WP_192193333.1 5193026..5194255(+) (pilC) [Variovorax sp. 38R]
ATGGCAACAGTGGCATCCTCCCGCTCCCAGGTCACGCACAAGGAATTCGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTACGTGGCGAGCTTCGCGCGGCCGGAGAGAACCAGGTCCAGGCGGCGCTGCGTCGCCAGGGCGTGCTGGCGT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGTCGATCAAGCCCAAGGACATTGCGATCTTCACGCGCCAGCTCGCG
ACCATGATGAAAGCCGGCGTGCCGCTGCTGCAGTCCTTCGACATCGTCGGCCGCGGCAACGCCAACCCGAGCGTCGCCAA
GCTGCTGAACGACATCCGCAGCGATGTCGAGACCGGCACGTCGCTGTCGGCTGCCTTCCGCAAGTTTCCGAAGTACTTCG
ACAACCTGTATTGCAACCTGGTGGAAGCCGGCGAGGCCGCCGGTATTCTGGAGGACCTGCTCGATCGCCTGGCCACCTAC
ATGGAGAAGACCGAGGCGATCAAGTCGAAGATCAAGTCGGCACTGATGTACCCGACTTCGGTGGTCGTTGTGGCCTTCAT
CGTGGTGGCGATCATCATGATCTTCGTGATCCCAGCATTCAAGGAGGTGTTCACTTCGTTCGGCGCCGACTTGCCCGCGC
CGACACTGATCGTGATGGCGATCAGCGAGTTTTTTGTGTCTTACTGGTGGTTGATCTTCGGCGTACTCGGCGGCGGCATC
TACTTCTTCCTTCAGGCTTGGAAGCGCAACGAACGCGTCCAGAAAGTCATGGACCGCCTGCTGCTGCGCCTTCCGATCTT
CGGCACGCTGATCGAGAAGTCCTGCATCGCCCGCTGGACCCGCACGCTCGCCACCATGTTCGCAGCTGGCGTGCCACTGG
TCGAGGCCCTCGACTCGGTGGGCGGTGCCTCGGGCAACTCGGTGTACGGCGACGCCACCGCCAAGATCCAGCAGGAAGTC
TCGACCGGCACCAGCCTGACGACGGCCATGACCAACGTCAACCTGTTCCCCTCGATGGTGATCCAGATGACCGCCATCGG
CGAAGAATCGGGCTCCATCGACCACATGCTCGGCAAGGCCGCAGACTTCTACGAGTCCGAGGTCGACGACATGGTCGCCG
GCCTCTCCAGCCTGATGGAGCCCATCATCATCGTGTTCCTGGGCGTCATCATCGGTGGCATCGTAGTGTCGATGTATCTG
CCCATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L8SD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.713

98.778

0.531
  pilG Neisseria gonorrhoeae MS11

53.149

97.066

0.516
  pilG Neisseria meningitidis 44/76-A

52.897

97.066

0.513
  pilC Acinetobacter baylyi ADP1

51.095

100

0.513
  pilC Legionella pneumophila strain ERS1305867

50.253

96.822

0.487
  pilC Acinetobacter baumannii D1279779

50.126

97.066

0.487
  pilC Vibrio cholerae strain A1552

41.206

97.311

0.401
  pilC Thermus thermophilus HB27

38.186

100

0.391
  pilC Vibrio campbellii strain DS40M4

39.303

98.289

0.386