Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IG196_RS24145 Genome accession   NZ_CP062121
Coordinates   5191268..5193001 (+) Length   577 a.a.
NCBI ID   WP_101488972.1    Uniprot ID   -
Organism   Variovorax sp. 38R     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5186268..5198001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG196_RS24110 (IG196_24110) - 5186269..5186862 (+) 594 WP_192193329.1 CNP1-like family protein -
  IG196_RS24115 (IG196_24115) proB 5186897..5188036 (-) 1140 WP_192193330.1 glutamate 5-kinase -
  IG196_RS24120 (IG196_24120) cgtA 5188117..5189193 (-) 1077 WP_192193331.1 Obg family GTPase CgtA -
  IG196_RS24125 (IG196_24125) rpmA 5189267..5189527 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  IG196_RS24130 (IG196_24130) rplU 5189540..5189851 (-) 312 WP_042578903.1 50S ribosomal protein L21 -
  IG196_RS24135 (IG196_24135) - 5190034..5191026 (+) 993 WP_192193332.1 polyprenyl synthetase family protein -
  IG196_RS24145 (IG196_24145) pilB 5191268..5193001 (+) 1734 WP_101488972.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IG196_RS24150 (IG196_24150) pilC 5193026..5194255 (+) 1230 WP_192193333.1 type II secretion system F family protein Machinery gene
  IG196_RS24155 (IG196_24155) - 5194255..5195220 (+) 966 WP_192193334.1 prepilin peptidase -
  IG196_RS24160 (IG196_24160) coaE 5195234..5195845 (+) 612 WP_192193335.1 dephospho-CoA kinase -
  IG196_RS24165 (IG196_24165) - 5195922..5197415 (+) 1494 WP_192193336.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62610.91 Da        Isoelectric Point: 5.7157

>NTDB_id=486142 IG196_RS24145 WP_101488972.1 5191268..5193001(+) (pilB) [Variovorax sp. 38R]
MAAAEIPVKETTQIALPGLARALVSAGKLAAKSAEEIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAASAAETI
NSIVGTEFEFDDVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMLCRVPIDIPR
EALLEAGFNEEDINGTWKPYRPVGCAACSGGYKGRVGIYQVMPITEAIQEIILRDGSALDIANQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=486142 IG196_RS24145 WP_101488972.1 5191268..5193001(+) (pilB) [Variovorax sp. 38R]
ATGGCCGCTGCCGAAATTCCCGTTAAAGAAACTACGCAGATCGCCCTTCCCGGCTTGGCGCGCGCCTTGGTGTCCGCCGG
CAAGCTTGCAGCGAAATCAGCCGAAGAGATCTATCAGAAGTCGCTGAGCGGGCGCACCAGTTTCATCGCCGAACTGACCG
GCACCGGCGCCGTCTCGGCCGCGGACCTCGCCCACACCCTGTCCAGCGCCTTCGGCGCCCCACTGCTCGACCTCGATGCC
ATCGATCACCAGCGCCTGCCCAAGGACCTGCTCGATCCGAAGCTCTGCCACGCCTATCGCATCGTCGTTCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCGACGGCCGACCCGTCGGACCAGCAGGCTGCGGAGAAGATCAAGTTCGCCTCGCAGATGG
GCGTCGACTGGGTCATCGCGGAGTACGACAAGCTCTCGCGCATGATCGAGGCCGCCGCAGCCAGTGCAGCCGAAACCATC
AACAGCATTGTCGGCACCGAGTTCGAATTCGACGATGTCACGGCTGACACCTCGGGCGACGCGAATGAGCAGGCCATCGC
CGAAGTCGAAGACGCACCCGTGGTGCGCTTCCTGCACAAGATGCTGCTCGACGCAGTGAGCATGCGGGCCTCGGACATTC
ACTTCGAGCCTTACGAACACCACTACCGGGTGCGCTTCCGGGTCGACGGTGAGCTGCGCGAAATCGCCTCCCCGCCCACC
GTCATCAAGGACAAGCTCGCCTCGCGCATCAAGGTCATTTCGCGACTCGACATCTCGGAAAAACGCGTGCCGCAAGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTCCGCGTGAGCACGCTGCCCACCCTGTTCGGCGAGAAGA
TCGTCGTGCGTATCCTGGACCCGAGCAGCGCGCGCATGGGCATCGACGCCCTCGGCTACGACGCCGACGAGAAGGAACGG
CTCCTCCATGCCATCGGCCGTCCCTACGGCATGGTGCTGGTGACCGGCCCCACGGGCTCGGGCAAAACCGTGTCGCTCTA
CACCTGCCTGAATCTGCTGAATCAGCCGGGCGTCAACATCGCAACGGCGGAAGACCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAGAAGGCCGGCCTCACCTTCGCGGCCGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGCCACTTGGTGCT
GTCGACGCTGCACACCAATGACGCCCCGACCACCCTCACGCGCATGCGCAACATGGGCATCGCACCGTTCAACATCGCGT
CGAGCGTGATCCTGATCACCGCGCAGCGCCTTGCGCGCCGGCTGTGCATGCTGTGCCGTGTGCCGATCGACATCCCGCGT
GAGGCTCTGCTCGAAGCCGGCTTCAACGAGGAAGACATCAACGGCACCTGGAAGCCATACCGCCCGGTCGGCTGCGCCGC
CTGCAGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATTACGGAAGCCATCCAGGAAATCATCCTGC
GCGACGGCAGTGCGCTGGACATCGCGAACCAATCCGAAGCCGAAGGCGTGCGCTCGCTGCGCCAGTCCGGCCTTCGCAAA
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTCGTGGCCTGCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-157)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.659

99.48

0.534
  pilB Acinetobacter baumannii D1279779

54.286

97.054

0.527
  pilF Neisseria gonorrhoeae MS11

52.847

97.4

0.515
  pilB Legionella pneumophila strain ERS1305867

48.85

97.92

0.478
  pilB Vibrio cholerae strain A1552

46.429

97.054

0.451
  pilB Vibrio campbellii strain DS40M4

46.085

97.4

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

97.227

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.254

97.574

0.383
  pilF Thermus thermophilus HB27

38.137

98.614

0.376