Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IF132_RS17285 Genome accession   NZ_CP061845
Coordinates   2493641..2495329 (-) Length   562 a.a.
NCBI ID   WP_039430449.1    Uniprot ID   A0A099LLY0
Organism   Vibrio navarrensis strain 2540-90     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2488641..2500329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF132_RS17255 (IF132_18320) rplS 2489153..2489506 (+) 354 WP_039430454.1 50S ribosomal protein L19 -
  IF132_RS17260 (IF132_18325) yacG 2489723..2489917 (-) 195 WP_039430453.1 DNA gyrase inhibitor YacG -
  IF132_RS17265 (IF132_18330) zapD 2490002..2490742 (-) 741 WP_039430452.1 cell division protein ZapD -
  IF132_RS17270 (IF132_18335) coaE 2490775..2491383 (-) 609 WP_039430451.1 dephospho-CoA kinase -
  IF132_RS17275 (IF132_18340) pilD 2491385..2492254 (-) 870 WP_039430450.1 prepilin peptidase Machinery gene
  IF132_RS17280 (IF132_18345) - 2492394..2493619 (-) 1226 Protein_2127 type II secretion system F family protein -
  IF132_RS17285 (IF132_18350) pilB 2493641..2495329 (-) 1689 WP_039430449.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IF132_RS17290 (IF132_18355) - 2495330..2495803 (-) 474 WP_039430448.1 pilin -
  IF132_RS17295 (IF132_18360) nadC 2496019..2496906 (-) 888 WP_039430447.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IF132_RS17300 (IF132_18365) ampD 2496999..2497562 (+) 564 WP_039430446.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IF132_RS17305 (IF132_18370) pdhR 2497972..2498742 (+) 771 WP_039430445.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62153.31 Da        Isoelectric Point: 6.2967

>NTDB_id=485058 IF132_RS17285 WP_039430449.1 2493641..2495329(-) (pilB) [Vibrio navarrensis strain 2540-90]
MIANLVTILRQATLLSPTQEQACLEHLNTSGASAPEVLLQLGFFQAEELTEHLSALFGLPPVELSHYDYQSVCNQLGLRE
LITRHQALPLQKNRSALILASADPTNLQAEDDFRFATGLQVEMVLADVKQLQGAIRRLYGRTLSQDNRSGLKEIHQDELA
NLVDVAADEVDNIEDLSQDSSPVSRFINQILLDAVRKGASDIHFEPYEAMYRVRLRCDGILVEVQQPPSHLSRRLSARLK
ILAKLDIAERRLPQDGRIKLKLNQSTAIDMRVSTLPTLFGEKIVLRLLDSSSSNLDIDKLGYSETQKQLYLNALRKPQGM
ILMTGPTGSGKTVSLYTGLNLLNRPEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCRHCKQPQHLSPALQELGLSQQD
GLYRANPQGCNECNQGYSGRVGIYEVMPFDDAMAEALIKGASVQSLEMLARKNGMLTLRESGLEKVKQGITSIEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=485058 IF132_RS17285 WP_039430449.1 2493641..2495329(-) (pilB) [Vibrio navarrensis strain 2540-90]
ATGATAGCTAACCTAGTCACGATTCTGCGCCAAGCTACCCTACTTAGCCCAACGCAAGAACAAGCCTGTTTGGAACATCT
TAACACTTCCGGTGCCAGTGCACCGGAAGTGTTGTTGCAACTGGGCTTTTTCCAAGCCGAAGAGCTGACCGAACATCTCA
GCGCGCTGTTTGGCTTGCCGCCCGTCGAGCTTAGCCACTATGACTACCAAAGCGTGTGCAACCAACTGGGGTTGCGCGAG
CTGATTACCCGCCACCAAGCCCTGCCGCTGCAAAAAAATCGCTCAGCGCTGATTCTCGCCAGTGCCGACCCCACCAATTT
GCAGGCCGAAGATGACTTTCGCTTTGCCACCGGGCTGCAAGTGGAAATGGTGTTGGCCGATGTTAAGCAGCTGCAAGGGG
CAATACGTCGCTTATATGGCCGCACGCTTTCTCAAGATAACCGCTCAGGACTGAAAGAGATCCACCAAGATGAGCTGGCC
AACTTAGTCGATGTGGCGGCCGATGAAGTCGACAATATTGAAGATCTCAGCCAAGACAGCTCCCCCGTCAGCCGTTTTAT
CAACCAGATCTTACTTGATGCAGTGCGTAAAGGCGCATCCGATATTCACTTTGAACCCTATGAAGCAATGTATCGCGTGC
GTTTACGCTGCGATGGCATTTTGGTCGAAGTGCAGCAGCCGCCAAGCCATTTGAGCCGTCGACTCTCCGCACGGCTTAAA
ATTCTCGCTAAGCTGGATATTGCCGAGCGGCGTTTACCGCAAGATGGGCGCATCAAACTCAAACTGAACCAAAGCACCGC
GATTGATATGCGCGTTTCTACCCTACCGACGCTGTTTGGTGAAAAAATCGTGTTGCGTCTGCTCGACAGCTCAAGTAGCA
ACTTAGACATTGATAAGCTCGGCTACAGCGAAACGCAAAAGCAGCTCTATCTCAACGCGCTGCGCAAACCGCAAGGGATG
ATCTTGATGACGGGCCCAACTGGCAGCGGCAAAACCGTTTCACTCTACACAGGTTTAAACCTACTCAATCGCCCTGAGGT
CAACATCTCTACCGCCGAAGATCCGGTAGAGATTAACTTGCCCGGCATTAACCAAGTGCAAGTACAGCCAAAAATCGGCT
TTGGCTTTGCGCAGGCACTGCGCTCATTTTTACGTCAAGACCCCGATATTGTCATGGTCGGTGAAATTCGCGATTTGGAA
ACCGCCGAAATAGCTATTAAAGCCGCGCAAACGGGGCATTTGGTGCTATCCACCCTACATACCAACTCGGCCGCAGAGAC
CATAGTACGCCTATCAAACATGGGCATTGCCAGCTTCAACCTCGCTTCTTCATTGAGCTTAATTATCGCTCAGCGCCTAG
CGCGTCGTTTGTGTCGCCATTGCAAGCAGCCACAACACCTTTCACCGGCATTACAAGAGCTAGGGTTAAGCCAGCAGGAT
GGGTTGTATCGTGCCAATCCTCAAGGGTGTAACGAATGCAATCAAGGGTATTCAGGGCGCGTCGGCATCTACGAAGTGAT
GCCCTTTGACGATGCCATGGCCGAGGCACTAATTAAAGGAGCCTCAGTACAAAGCTTGGAAATGCTCGCCAGAAAAAATG
GCATGCTGACGCTTCGAGAATCTGGGCTAGAAAAGGTCAAGCAAGGCATCACCAGCATTGAAGAGCTGCAACGCGTGCTC
TACCTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A099LLY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

78.114

100

0.781
  pilB Vibrio campbellii strain DS40M4

76.512

100

0.765
  pilB Vibrio cholerae strain A1552

72.824

100

0.73
  pilB Acinetobacter baumannii D1279779

52.713

91.815

0.484
  pilB Legionella pneumophila strain ERS1305867

51.423

93.772

0.482
  pilB Acinetobacter baylyi ADP1

50.969

91.815

0.468
  pilF Neisseria gonorrhoeae MS11

45.775

100

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.821

100

0.399
  pilF Thermus thermophilus HB27

38.503

99.822

0.384
  pilB Haemophilus influenzae 86-028NP

42.623

86.833

0.37
  pilB Haemophilus influenzae Rd KW20

42.975

86.121

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.786

99.644

0.367