Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IEE84_RS01475 Genome accession   NZ_CP061739
Coordinates   336920..337363 (+) Length   147 a.a.
NCBI ID   WP_191114648.1    Uniprot ID   -
Organism   Psychrobacter sp. 28M-43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331920..342363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEE84_RS01465 (IEE84_01465) - 335515..336021 (+) 507 WP_191114646.1 type IV pilin protein -
  IEE84_RS01470 (IEE84_01470) - 336099..336551 (+) 453 WP_323969846.1 type IV pilin protein -
  IEE84_RS01475 (IEE84_01475) pilA 336920..337363 (+) 444 WP_191114648.1 pilin Machinery gene
  IEE84_RS01480 (IEE84_01480) - 337438..339045 (+) 1608 WP_191114649.1 O-antigen ligase family protein -
  IEE84_RS13200 (IEE84_01485) - 339181..339627 (+) 447 WP_191114650.1 pilin -
  IEE84_RS01490 (IEE84_01490) fdxA 339715..340038 (-) 324 WP_101205516.1 ferredoxin FdxA -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 14852.86 Da        Isoelectric Point: 4.3307

>NTDB_id=484423 IEE84_RS01475 WP_191114648.1 336920..337363(+) (pilA) [Psychrobacter sp. 28M-43]
MNAQKGFTLIELMIVIAIIGILAAIAIPAYQGYTAKSQVAAGLAEISPAKTNAEAKLAEGISDTLTTADLGIQGSTSRCS
AITSSVIATGKSTIACTLVGTSSINGDTIQWTRDGDTDSAIGAWTCSTNVPEESAPKSCTVGLVTPS

Nucleotide


Download         Length: 444 bp        

>NTDB_id=484423 IEE84_RS01475 WP_191114648.1 336920..337363(+) (pilA) [Psychrobacter sp. 28M-43]
ATGAACGCTCAAAAAGGTTTTACCCTAATCGAACTAATGATCGTTATCGCTATTATCGGTATCTTGGCTGCGATCGCTAT
TCCTGCTTATCAAGGCTATACCGCTAAATCACAAGTAGCTGCGGGATTAGCAGAGATTTCACCAGCGAAAACTAATGCTG
AAGCAAAGTTGGCAGAGGGTATTTCTGATACTCTTACTACCGCTGATCTTGGTATACAGGGTTCGACTAGCCGTTGCTCT
GCAATTACATCTAGTGTAATAGCGACTGGCAAATCTACAATTGCATGTACTTTAGTAGGTACTTCTTCCATTAATGGTGA
TACCATTCAATGGACTCGTGATGGAGATACAGACAGTGCAATTGGTGCGTGGACATGCTCAACTAATGTTCCTGAAGAGT
CAGCACCTAAATCTTGTACAGTTGGTCTTGTTACTCCTAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

51.034

98.639

0.503
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.622

100

0.469
  pilA Vibrio cholerae strain A1552

46.622

100

0.469
  pilA Vibrio cholerae C6706

46.622

100

0.469
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.775

96.599

0.442
  pilA Pseudomonas aeruginosa PAK

40.523

100

0.422
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.571

95.238

0.415
  pilA Vibrio parahaemolyticus RIMD 2210633

44.961

87.755

0.395
  pilA Ralstonia pseudosolanacearum GMI1000

42.857

85.714

0.367