Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IC797_RS01740 Genome accession   NZ_CP061556
Coordinates   369474..369911 (+) Length   145 a.a.
NCBI ID   WP_191012237.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364474..374911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC797_RS01720 (IC797_01720) - 365870..366757 (+) 888 WP_191012240.1 metal-dependent hydrolase -
  IC797_RS01725 (IC797_01725) - 366888..367478 (+) 591 WP_002051712.1 LemA family protein -
  IC797_RS01730 (IC797_01730) - 367500..368579 (+) 1080 WP_191012239.1 YgcG family protein -
  IC797_RS01735 (IC797_01735) - 368573..369133 (+) 561 WP_191012238.1 TPM domain-containing protein -
  IC797_RS01740 (IC797_01740) pilA 369474..369911 (+) 438 WP_191012237.1 pilin Machinery gene
  IC797_RS01745 (IC797_01745) - 369972..371282 (+) 1311 WP_191105888.1 O-antigen ligase -
  IC797_RS01750 (IC797_01750) - 371396..373024 (+) 1629 WP_191012235.1 PglL family O-oligosaccharyltransferase -
  IC797_RS01755 (IC797_01755) bfr 373066..373530 (-) 465 WP_000678123.1 bacterioferritin -
  IC797_RS01760 (IC797_01760) - 373775..373969 (-) 195 WP_004705220.1 bacterioferritin-associated ferredoxin -
  IC797_RS01765 (IC797_01765) - 374178..374561 (-) 384 WP_191012234.1 RidA family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14974.99 Da        Isoelectric Point: 7.5909

>NTDB_id=483153 IC797_RS01740 WP_191012237.1 369474..369911(+) (pilA) [Acinetobacter seifertii strain AS74]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQVSTGLTDITAGKANTETKLAEGLTNKLTNATDVGLQASSNAC
SSISVSVDTTGLTTIECTLQGTSQISGKKISWERTADSATAGTTGTWRCKTDVADNLRPKSCGAS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=483153 IC797_RS01740 WP_191012237.1 369474..369911(+) (pilA) [Acinetobacter seifertii strain AS74]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTTATGATCGTGGTTGCGATTATTGGTATTTTAGCTGCAATTGCGAT
TCCAGCTTATCAAAACTACATTGCTAAATCTCAAGTAAGTACAGGACTAACTGATATTACCGCTGGTAAAGCTAATACTG
AAACTAAATTAGCAGAAGGTTTAACTAATAAATTAACAAATGCTACTGATGTAGGCTTGCAAGCTTCAAGCAATGCTTGT
TCTTCAATTAGTGTTTCAGTTGATACTACTGGATTAACAACAATTGAATGTACGTTACAAGGCACTTCACAAATTAGTGG
TAAGAAAATTTCTTGGGAGCGAACAGCTGACAGTGCTACAGCAGGAACTACAGGTACATGGCGTTGTAAAACTGATGTAG
CTGATAATTTACGTCCTAAATCATGTGGCGCATCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.98

100

0.497
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.39

97.241

0.441
  pilA Pseudomonas aeruginosa PAK

40.26

100

0.428
  pilA Vibrio cholerae C6706

41.892

100

0.428
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.892

100

0.428
  pilA Vibrio cholerae strain A1552

41.892

100

0.428
  comP Acinetobacter baylyi ADP1

39.869

100

0.421
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.254

97.931

0.414
  pilA/pilA1 Eikenella corrodens VA1

35.526

100

0.372