Detailed information NTG0047709

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLH_RS11745
• Genome accession: NC_017492
• Coordinates: 2257412..2257837 (-)
• Length: 141 a.a.
• NCBI ID: WP_014735174.1
• Uniprot ID: -
• Organism: Lactococcus cremoris subsp. cremoris A76
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2252555..2260656	2257412..2257837	within	0

Gene organization within MGE regions

Location: 2252555..2260656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLH_RS11720 (llh_12275)	-	2254015..2254824 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLH_RS11725 (llh_12280)	-	2254817..2255554 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLH_RS11730 (llh_12285)	-	2255733..2256575 (-)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLH_RS11735 (llh_12290)	-	2256572..2257009 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLH_RS11740 (llh_12295)	comGG	2257089..2257388 (-)	300	WP_011677181.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLH_RS11745	comGF	2257412..2257837 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLH_RS11750 (llh_12305)	comGE	2257821..2258057 (-)	237	WP_014573335.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLH_RS14595 (llh_12310)	comGD	2258089..2258277 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLH_RS11760 (llh_12320)	comGC	2258479..2258829 (-)	351	WP_050574401.1	competence type IV pilus major pilin ComGC	Machinery gene
LLH_RS11765 (llh_12325)	comGB	2258874..2259899 (-)	1026	WP_050574400.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLH_RS11770 (llh_12330)	-	2259799..2260620 (-)	822	Protein_2274	ATPase, T2SS/T4P/T4SS family	-

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16210.78 Da
• Isoelectric Point: 8.4549

>NTDB_id=47709 LLH_RS11745 WP_014735174.1 2257412..2257837(-) (comGF) [Lactococcus cremoris subsp. cremoris A76]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=47709 LLH_RS11745 WP_014735174.1 2257412..2257837(-) (comGF) [Lactococcus cremoris subsp. cremoris A76]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	100	100	1
comYF	Streptococcus mutans UA140	43.972	100	0.44
comYF	Streptococcus mutans UA159	43.262	100	0.433
comGF/cglF	Streptococcus mitis NCTC 12261	39.286	99.291	0.39
comGF/cglF	Streptococcus mitis SK321	39.286	99.291	0.39
comGF/cglF	Streptococcus pneumoniae Rx1	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae D39	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae R6	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae TIGR4	37.857	99.291	0.376