Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LLH_RS11765 Genome accession   NC_017492
Coordinates   2258874..2259899 (-) Length   341 a.a.
NCBI ID   WP_050574400.1    Uniprot ID   -
Organism   Lactococcus cremoris subsp. cremoris A76     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2252555..2260656 2258874..2259899 within 0
IS/Tn 2260723..2261613 2258874..2259899 flank 824


Gene organization within MGE regions


Location: 2252555..2261613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLH_RS11720 (llh_12275) - 2254015..2254824 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LLH_RS11725 (llh_12280) - 2254817..2255554 (-) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLH_RS11730 (llh_12285) - 2255733..2256575 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLH_RS11735 (llh_12290) - 2256572..2257009 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLH_RS11740 (llh_12295) comGG 2257089..2257388 (-) 300 WP_011677181.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLH_RS11745 comGF 2257412..2257837 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLH_RS11750 (llh_12305) comGE 2257821..2258057 (-) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLH_RS14595 (llh_12310) comGD 2258089..2258277 (-) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLH_RS11760 (llh_12320) comGC 2258479..2258829 (-) 351 WP_050574401.1 competence type IV pilus major pilin ComGC Machinery gene
  LLH_RS11765 (llh_12325) comGB 2258874..2259899 (-) 1026 WP_050574400.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLH_RS11770 (llh_12330) - 2259799..2260620 (-) 822 Protein_2274 ATPase, T2SS/T4P/T4SS family -
  LLH_RS11775 (llh_12335) - 2260723..2261613 (+) 891 WP_014573340.1 IS982-like element IS982B family transposase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38411.13 Da        Isoelectric Point: 10.0626

>NTDB_id=47712 LLH_RS11765 WP_050574400.1 2258874..2259899(-) (comGB) [Lactococcus cremoris subsp. cremoris A76]
MQTDILRLLRPKGKKLALIKQAKLIQLMGNLLNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILDSLSFSK
NVVTQLALVEVHGNLVGTMQLVELHLKKQLKVKKKLIEVATYPVVLLVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLP
LVFFSVGALIVLLTSLSIIIFKHLSALTNFTFLVKVPLVSSFIRLYLTAYFSREWGNLIAQGVELRQIIELMKKQKSRIF
AEVGIKLDLGLKAGQNFEQAVSHFSIFLPELSLMIEYGAIKDKLGLELSVYADECWEQFFSKIDRLMQLIQPLIFIFVAL
MIVLLYAAMSLPIYSNMGSGI

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=47712 LLH_RS11765 WP_050574400.1 2258874..2259899(-) (comGB) [Lactococcus cremoris subsp. cremoris A76]
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAG
AGTTTGTATTCAAAATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGATAGTCTCTCTTTTTCTAAA
AATGTTGTGACACAACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAA
AAAGCAGCTTAAGGTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTGGTATTTCTCATTGGAATTA
TGTGGGGCTTGAAAAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCG
CTAGTATTTTTTTCTGTTGGTGCTTTAATCGTTTTACTGACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACT
CACTAATTTTACTTTTTTGGTTAAAGTTCCATTAGTAAGTTCTTTTATCCGCTTGTATTTGACCGCTTATTTCTCCAGAG
AATGGGGAAATTTAATTGCTCAAGGAGTTGAATTGCGACAAATAATAGAATTGATGAAAAAGCAGAAAAGTCGCATTTTT
GCGGAAGTTGGAATTAAACTTGATTTGGGTTTGAAGGCTGGTCAGAATTTTGAGCAAGCTGTCAGTCATTTTTCGATTTT
TTTACCAGAACTTTCTTTGATGATTGAGTACGGCGCGATTAAAGATAAATTAGGGTTGGAGCTTTCTGTTTATGCTGATG
AATGTTGGGAACAATTCTTTTCTAAGATAGACCGTCTAATGCAATTAATACAACCCCTTATTTTTATTTTTGTTGCTTTG
ATGATTGTCTTATTGTATGCAGCGATGTCGTTACCCATTTATTCTAATATGGGTTCTGGAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(25-143)

Type II secretion system (T2SS), protein F (T2SSF)

(211-332)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

98.24

100

0.982
  comYB Streptococcus gordonii str. Challis substr. CH1

51.632

98.827

0.51
  comGB/cglB Streptococcus mitis SK321

50.599

97.947

0.496
  comGB/cglB Streptococcus pneumoniae TIGR4

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae Rx1

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae D39

50.299

97.947

0.493
  comGB/cglB Streptococcus pneumoniae R6

50.299

97.947

0.493
  comGB/cglB Streptococcus mitis NCTC 12261

49.701

97.947

0.487
  comYB Streptococcus mutans UA140

51.592

92.082

0.475
  comYB Streptococcus mutans UA159

50.955

92.082

0.469

Multiple sequence alignment