Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   H8N02_RS04385 Genome accession   NZ_CP060413
Coordinates   937846..939069 (-) Length   407 a.a.
NCBI ID   WP_054067379.1    Uniprot ID   A0A0W7YZY7
Organism   Comamonas kerstersii strain 3132976     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 932846..944069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8N02_RS04355 (H8N02_04355) - 933802..934734 (+) 933 WP_151042671.1 ATP-binding protein -
  H8N02_RS04360 (H8N02_04360) - 934747..935211 (+) 465 WP_058880033.1 NUDIX domain-containing protein -
  H8N02_RS04365 (H8N02_04365) - 935297..935500 (-) 204 WP_054067375.1 DNA gyrase inhibitor YacG -
  H8N02_RS04370 (H8N02_04370) zapD 935528..936283 (-) 756 WP_077345505.1 cell division protein ZapD -
  H8N02_RS04375 (H8N02_04375) coaE 936366..936974 (-) 609 WP_058880035.1 dephospho-CoA kinase -
  H8N02_RS04380 (H8N02_04380) - 936977..937846 (-) 870 WP_058880036.1 prepilin peptidase -
  H8N02_RS04385 (H8N02_04385) pilC 937846..939069 (-) 1224 WP_054067379.1 type II secretion system F family protein Machinery gene
  H8N02_RS04390 (H8N02_04390) pilB 939151..940893 (-) 1743 WP_258536234.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H8N02_RS04395 (H8N02_04395) - 941043..941972 (-) 930 WP_210607678.1 polyprenyl synthetase family protein -
  H8N02_RS04400 (H8N02_04400) rplU 942184..942495 (+) 312 WP_054067382.1 50S ribosomal protein L21 -
  H8N02_RS04405 (H8N02_04405) rpmA 942514..942774 (+) 261 WP_054067383.1 50S ribosomal protein L27 -
  H8N02_RS04410 (H8N02_04410) cgtA 942934..944007 (+) 1074 WP_210607254.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44734.82 Da        Isoelectric Point: 9.3792

>NTDB_id=475805 H8N02_RS04385 WP_054067379.1 937846..939069(-) (pilC) [Comamonas kerstersii strain 3132976]
MANATIGRAPAKEFIFEWVGKDRQGKELRGEMRATGEAQVQASLRRQGIFMTKLKKRALRAGKKIKPKDIAVFTRQMATM
MKAGVPLLQAFDIVGRGNPNPSVTKLLNDIRQDVETGTSLSVAFRKHPMYFDALYCNLVEAGEAAGILETLMDRLATYME
KTEAIKGKIKSALMYPTSVVLVAFIVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMSISEFFVDYWWLIFGVLGGGFYF
FMQAWKRSPKMQRVMDRLLLKMPIFGVLIDKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNSVYKEATDRIQHEVAT
GSSLNVAMANANVFPSMVLQMCAIGEESGAIDHMLGKAADFYEQEVDEMVAGLSSLMEPIIIVFLGGIIGSIVVAMYLPI
FKLGQVV

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=475805 H8N02_RS04385 WP_054067379.1 937846..939069(-) (pilC) [Comamonas kerstersii strain 3132976]
ATGGCAAACGCAACAATTGGCCGAGCGCCAGCCAAGGAATTCATCTTTGAATGGGTAGGCAAAGACCGGCAGGGCAAAGA
GCTGCGCGGCGAAATGCGAGCCACAGGAGAAGCACAGGTCCAGGCGTCGCTGCGCCGCCAGGGTATCTTCATGACCAAGC
TGAAAAAGCGCGCCCTGCGGGCTGGCAAGAAGATCAAGCCCAAGGACATTGCCGTCTTCACGCGCCAGATGGCCACCATG
ATGAAGGCGGGCGTGCCCTTGCTGCAGGCCTTCGACATCGTGGGCCGCGGCAACCCCAACCCCAGCGTGACCAAGCTGCT
CAACGACATCCGTCAGGATGTAGAGACCGGCACTTCACTGAGTGTGGCGTTTCGCAAGCACCCCATGTACTTCGATGCGC
TGTACTGCAACCTGGTCGAAGCGGGTGAAGCCGCAGGTATTCTGGAAACCCTGATGGATCGCCTGGCCACCTATATGGAA
AAGACCGAGGCCATCAAGGGCAAGATCAAATCAGCCCTGATGTACCCCACCTCGGTCGTGCTGGTGGCCTTTATTGTGGT
GACGGTGATCATGATCTTTGTGATCCCTGCCTTCAAGGAAGTCTTTACCTCCTTTGGTGCCGATCTGCCCGCCCCCACCC
TGCTGGTAATGTCCATCAGCGAGTTCTTTGTGGACTACTGGTGGCTGATCTTTGGCGTGCTCGGTGGAGGTTTTTACTTT
TTCATGCAGGCCTGGAAGCGCAGCCCCAAGATGCAGCGCGTCATGGACCGCCTGCTGCTGAAAATGCCCATCTTTGGCGT
GCTGATCGACAAATCCTGCGTGGCGCGCTGGACGCGTACGCTCTCGACCATGTTTGCCGCAGGCGTGCCCCTGGTGGAGG
CACTGGACTCTGTGGGCGGCGCTTCCGGCAACAGCGTCTACAAGGAGGCCACCGACCGCATCCAGCATGAAGTAGCCACC
GGCTCCAGCCTGAACGTGGCCATGGCCAATGCCAATGTCTTTCCATCCATGGTGCTGCAGATGTGCGCCATTGGCGAGGA
GTCGGGTGCCATTGACCACATGCTGGGCAAGGCTGCCGACTTCTACGAACAAGAGGTCGATGAGATGGTGGCCGGGCTTT
CCAGCCTGATGGAGCCCATCATCATCGTGTTTCTGGGTGGCATCATCGGCAGCATCGTGGTGGCCATGTACCTGCCTATC
TTCAAATTGGGACAAGTGGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W7YZY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.138

98.034

0.541
  pilG Neisseria gonorrhoeae MS11

53.149

97.543

0.518
  pilG Neisseria meningitidis 44/76-A

52.897

97.543

0.516
  pilC Legionella pneumophila strain ERS1305867

50.758

97.297

0.494
  pilC Acinetobacter baylyi ADP1

49.497

97.789

0.484
  pilC Acinetobacter baumannii D1279779

47.859

97.543

0.467
  pilC Vibrio cholerae strain A1552

41.414

97.297

0.403
  pilC Vibrio campbellii strain DS40M4

40.5

98.28

0.398
  pilC Thermus thermophilus HB27

39.098

98.034

0.383