Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H8N02_RS04390 Genome accession   NZ_CP060413
Coordinates   939151..940893 (-) Length   580 a.a.
NCBI ID   WP_258536234.1    Uniprot ID   -
Organism   Comamonas kerstersii strain 3132976     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 934151..945893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8N02_RS04360 (H8N02_04360) - 934747..935211 (+) 465 WP_058880033.1 NUDIX domain-containing protein -
  H8N02_RS04365 (H8N02_04365) - 935297..935500 (-) 204 WP_054067375.1 DNA gyrase inhibitor YacG -
  H8N02_RS04370 (H8N02_04370) zapD 935528..936283 (-) 756 WP_077345505.1 cell division protein ZapD -
  H8N02_RS04375 (H8N02_04375) coaE 936366..936974 (-) 609 WP_058880035.1 dephospho-CoA kinase -
  H8N02_RS04380 (H8N02_04380) - 936977..937846 (-) 870 WP_058880036.1 prepilin peptidase -
  H8N02_RS04385 (H8N02_04385) pilC 937846..939069 (-) 1224 WP_054067379.1 type II secretion system F family protein Machinery gene
  H8N02_RS04390 (H8N02_04390) pilB 939151..940893 (-) 1743 WP_258536234.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H8N02_RS04395 (H8N02_04395) - 941043..941972 (-) 930 WP_210607678.1 polyprenyl synthetase family protein -
  H8N02_RS04400 (H8N02_04400) rplU 942184..942495 (+) 312 WP_054067382.1 50S ribosomal protein L21 -
  H8N02_RS04405 (H8N02_04405) rpmA 942514..942774 (+) 261 WP_054067383.1 50S ribosomal protein L27 -
  H8N02_RS04410 (H8N02_04410) cgtA 942934..944007 (+) 1074 WP_210607254.1 Obg family GTPase CgtA -
  H8N02_RS04415 (H8N02_04415) proB 944089..945225 (+) 1137 WP_054067385.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63180.58 Da        Isoelectric Point: 6.2054

>NTDB_id=475806 H8N02_RS04390 WP_258536234.1 939151..940893(-) (pilB) [Comamonas kerstersii strain 3132976]
MTAVTNAPSLAPTGTMQLPGLARALVSAGQLSEATAREVALKATASKSSFVAQLVSSGHFSAAELAHQISLIFSTPLLDL
NAIDPLLLPKDLLDPKLCTSHHVLPLCKRGNRLIVATAAPTDKEATEKIKFASQMGIDWIIAEHDKLVRLIESAHKSVTE
ALDTYIADDEFQFDDTKEAPQQEDKDSSASDVEDAPVVKFLHKMLIDAVNMRASDLHFEPYEHNYRVRFRVDGELREIAS
PPITIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPERVIDFRVSTLPTLFGEKIVIRILDPNSAKMGIDALGYEPEE
KARLLDAIHRPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNISTAEDPSEINMPGVNQVNVNDKAGLTFAAALKAFLRQ
DPDVIMVGEIRDLETADIAIKAAQTGHMVLSTLHTNDAPTTLTRLRNMGVAPFNIASSVILITAQRLARRLCPQCKQPHD
VPTEALLAAGFEESQLDGSWVPYKAVGCSACNNGYKGRVGIYQVMPISEEIQQIILRDGSALDIANQAKREGVKSLRESG
LDKVRQGVTSLEEVLAVTNL

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=475806 H8N02_RS04390 WP_258536234.1 939151..940893(-) (pilB) [Comamonas kerstersii strain 3132976]
ATGACTGCTGTTACCAACGCCCCATCACTTGCACCGACTGGCACCATGCAGCTTCCGGGCTTAGCCCGTGCTTTAGTCAG
TGCTGGGCAGCTATCAGAAGCTACTGCTCGTGAAGTCGCCCTCAAGGCCACTGCCAGCAAAAGCAGCTTTGTAGCGCAGC
TGGTCAGCAGCGGCCATTTCTCTGCCGCAGAACTAGCCCACCAGATCTCGCTGATCTTCAGCACGCCACTGCTGGATCTG
AACGCCATTGACCCGCTGCTGCTGCCCAAGGATCTGCTCGACCCCAAGCTGTGCACCAGCCACCATGTGCTGCCGCTGTG
CAAGCGCGGCAACCGCCTGATCGTGGCCACGGCGGCCCCCACCGACAAGGAAGCCACGGAAAAAATCAAATTTGCCTCTC
AGATGGGCATTGACTGGATCATTGCCGAGCACGACAAACTGGTCAGACTCATTGAGAGCGCACACAAAAGCGTCACGGAA
GCGCTGGACACTTATATTGCAGACGACGAATTCCAGTTCGACGATACCAAGGAAGCTCCCCAGCAGGAGGACAAGGACAG
CAGCGCTTCGGACGTAGAAGATGCTCCCGTTGTCAAATTCCTGCACAAGATGCTGATCGATGCCGTCAACATGCGCGCAT
CGGACCTGCACTTTGAGCCGTATGAGCACAACTACCGCGTGCGCTTTCGTGTGGACGGTGAGCTGCGTGAAATTGCCTCG
CCACCTATCACCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGATTTCACGTCTGGACATTTCGGAAAAACGCGTGCC
ACAGGATGGCCGCATGAAGCTCAAGATCGGCCCTGAACGGGTGATTGACTTCCGTGTGTCCACCCTGCCCACGCTGTTTG
GCGAAAAAATCGTCATTCGTATCTTGGACCCCAACAGCGCCAAGATGGGCATTGATGCCCTGGGCTATGAGCCCGAAGAA
AAAGCACGCCTGCTCGATGCCATCCATCGTCCCTACGGCATGATTCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTCTACACCTGCCTGAACATCCTCAATCAGCCAGGCGTCAACATCTCGACCGCAGAAGACCCTTCCGAAATCAACA
TGCCGGGGGTCAACCAGGTCAACGTCAATGACAAGGCGGGCCTGACCTTTGCTGCTGCGCTCAAGGCGTTTTTGCGTCAG
GACCCGGACGTGATCATGGTCGGTGAAATCCGTGACCTGGAAACTGCAGACATCGCCATCAAGGCCGCACAGACCGGCCA
CATGGTGCTCTCGACCCTGCACACCAACGATGCCCCCACCACGCTGACGCGTCTGCGCAACATGGGGGTAGCACCGTTCA
ACATCGCCTCCAGCGTCATCCTGATTACGGCCCAGCGTCTGGCACGTCGCCTGTGCCCCCAATGCAAGCAGCCGCACGAT
GTCCCGACGGAAGCGCTGCTGGCCGCAGGCTTTGAAGAAAGCCAGCTCGACGGCTCCTGGGTGCCGTACAAGGCCGTAGG
CTGCAGCGCCTGCAACAATGGCTACAAGGGCCGCGTCGGCATCTACCAGGTCATGCCCATCAGCGAAGAAATACAGCAAA
TCATCCTGCGTGATGGCTCCGCACTGGACATTGCCAACCAGGCCAAGCGCGAAGGCGTCAAATCCTTGCGTGAATCAGGT
TTGGACAAGGTCCGGCAAGGCGTTACCTCGCTGGAAGAAGTTCTGGCAGTTACCAACCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.782

100

0.566
  pilB Acinetobacter baumannii D1279779

56.383

97.241

0.548
  pilF Neisseria gonorrhoeae MS11

53.641

97.069

0.521
  pilB Legionella pneumophila strain ERS1305867

50.174

98.966

0.497
  pilB Vibrio cholerae strain A1552

48.294

96.034

0.464
  pilB Vibrio campbellii strain DS40M4

46.099

97.241

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.52

96.207

0.438
  pilF Thermus thermophilus HB27

40.672

92.414

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.112

92.414

0.371