Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   H7F36_RS10300 Genome accession   NZ_CP060296
Coordinates   2180360..2181580 (-) Length   406 a.a.
NCBI ID   WP_187054576.1    Uniprot ID   A0A7G8W596
Organism   Variovorax sp. PAMC28562     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2175360..2186580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F36_RS10280 (H7F36_10280) - 2176193..2177203 (-) 1011 WP_187054572.1 hypothetical protein -
  H7F36_RS10285 (H7F36_10285) - 2177206..2178699 (-) 1494 WP_187054573.1 MBOAT family O-acyltransferase -
  H7F36_RS10290 (H7F36_10290) coaE 2178780..2179382 (-) 603 WP_187054574.1 dephospho-CoA kinase -
  H7F36_RS10295 (H7F36_10295) - 2179401..2180360 (-) 960 WP_187054575.1 prepilin peptidase -
  H7F36_RS10300 (H7F36_10300) pilC 2180360..2181580 (-) 1221 WP_187054576.1 type II secretion system F family protein Machinery gene
  H7F36_RS10305 (H7F36_10305) pilB 2181602..2183335 (-) 1734 WP_187054577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7F36_RS10315 (H7F36_10315) - 2183579..2184577 (-) 999 WP_187054578.1 polyprenyl synthetase family protein -
  H7F36_RS10320 (H7F36_10320) rplU 2184764..2185075 (+) 312 WP_187054579.1 50S ribosomal protein L21 -
  H7F36_RS10325 (H7F36_10325) rpmA 2185088..2185357 (+) 270 WP_187054580.1 50S ribosomal protein L27 -
  H7F36_RS10330 (H7F36_10330) cgtA 2185426..2186493 (+) 1068 WP_187054581.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44170.79 Da        Isoelectric Point: 9.6629

>NTDB_id=475542 H7F36_RS10300 WP_187054576.1 2180360..2181580(-) (pilC) [Variovorax sp. PAMC28562]
MATAASTRTLKEFVFEWEGKDRNGKAVRGEVRAAGENQVQAALRRQGVLASKIKKRRMRSGKSIKPKDIAIFTRQLATMM
KAGVPLLQSFDIVGRGNANPSVSKLLNDVRSDVETGTSLSSAFRKFPKYFDNLYCNLVEAGEAAGILEELLDRLATYMEK
TEAIKSKIKSALMYPASVVVVAFVVVAIIMIFVIPAFKSVFSSFGADLPAPTLIVMGISEFFVAYWWLIFGVIGGGLYFF
MQAWKRNERVQKVMDRVLLKLPIFGALIDKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNSVYADATVKIQQEVSTG
TSLTSAMTNANLFPSMVLQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVLIGGIVVSMYLPIF
KLGQVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=475542 H7F36_RS10300 WP_187054576.1 2180360..2181580(-) (pilC) [Variovorax sp. PAMC28562]
ATGGCCACCGCCGCATCGACACGCACTCTTAAAGAATTCGTCTTCGAATGGGAAGGCAAGGACCGCAATGGCAAGGCTGT
CCGCGGCGAAGTGCGCGCGGCGGGCGAGAACCAAGTCCAGGCCGCGTTGCGCCGCCAGGGCGTGCTCGCATCGAAGATCA
AGAAGCGCCGGATGCGCTCCGGCAAGTCGATCAAGCCTAAAGACATCGCCATCTTCACGCGCCAACTCGCGACCATGATG
AAAGCAGGCGTGCCGCTTTTGCAGTCGTTCGACATCGTGGGCCGAGGCAACGCCAACCCCAGCGTTTCCAAACTGCTCAA
CGACGTGCGCAGCGACGTGGAAACAGGCACTTCGCTCTCCTCGGCGTTCCGCAAGTTCCCGAAGTATTTTGACAACCTCT
ACTGCAATCTGGTCGAGGCAGGTGAAGCGGCCGGTATTTTGGAGGAGTTGCTCGACCGCCTGGCCACTTACATGGAAAAG
ACCGAAGCGATCAAGTCGAAGATTAAATCGGCTTTGATGTATCCCGCATCGGTCGTTGTAGTGGCCTTCGTGGTGGTTGC
GATCATCATGATTTTCGTGATCCCGGCGTTCAAGTCGGTTTTCAGTTCATTCGGGGCGGACCTGCCGGCTCCCACGTTGA
TCGTGATGGGGATCAGCGAATTTTTCGTGGCTTACTGGTGGTTGATCTTCGGCGTCATCGGCGGCGGTCTGTACTTTTTC
ATGCAGGCATGGAAGCGCAACGAACGCGTTCAAAAGGTGATGGACCGGGTGCTGCTGAAGTTGCCTATCTTCGGTGCGCT
TATCGACAAATCCTGCGTGGCACGATGGACTCGCACGTTGTCGACGATGTTCGCAGCCGGCGTGCCTTTGGTCGAGGCGC
TCGATTCCGTGGGGGGCGCTTCGGGCAACTCGGTGTACGCCGACGCCACCGTCAAGATTCAGCAAGAGGTTTCTACCGGC
ACCAGCCTGACGAGCGCAATGACCAACGCGAACCTGTTCCCATCGATGGTTTTGCAGATGACGGCCATCGGCGAAGAGTC
GGGCTCCATCGATCACATGCTCGGCAAGGCGGCCGACTTCTACGAATCCGAAGTCGACGACATGGTCGCTGGCCTGTCGA
GCCTGATGGAGCCCATCATCATCGTGTTTTTGGGCGTGCTCATCGGCGGCATCGTGGTGTCCATGTACCTGCCCATCTTC
AAGCTCGGTCAAGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8W596

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.455

99.507

0.542
  pilG Neisseria gonorrhoeae MS11

51.515

97.537

0.502
  pilG Neisseria meningitidis 44/76-A

51.263

97.537

0.5
  pilC Acinetobacter baylyi ADP1

50.754

98.03

0.498
  pilC Legionella pneumophila strain ERS1305867

50.503

98.03

0.495
  pilC Acinetobacter baumannii D1279779

49.622

97.783

0.485
  pilC Vibrio cholerae strain A1552

41.032

100

0.411
  pilC Thermus thermophilus HB27

37.767

100

0.392
  pilC Vibrio campbellii strain DS40M4

39.303

99.015

0.389