Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7F36_RS10305 Genome accession   NZ_CP060296
Coordinates   2181602..2183335 (-) Length   577 a.a.
NCBI ID   WP_187054577.1    Uniprot ID   A0A7G8W597
Organism   Variovorax sp. PAMC28562     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2176602..2188335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F36_RS10285 (H7F36_10285) - 2177206..2178699 (-) 1494 WP_187054573.1 MBOAT family O-acyltransferase -
  H7F36_RS10290 (H7F36_10290) coaE 2178780..2179382 (-) 603 WP_187054574.1 dephospho-CoA kinase -
  H7F36_RS10295 (H7F36_10295) - 2179401..2180360 (-) 960 WP_187054575.1 prepilin peptidase -
  H7F36_RS10300 (H7F36_10300) pilC 2180360..2181580 (-) 1221 WP_187054576.1 type II secretion system F family protein Machinery gene
  H7F36_RS10305 (H7F36_10305) pilB 2181602..2183335 (-) 1734 WP_187054577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7F36_RS10315 (H7F36_10315) - 2183579..2184577 (-) 999 WP_187054578.1 polyprenyl synthetase family protein -
  H7F36_RS10320 (H7F36_10320) rplU 2184764..2185075 (+) 312 WP_187054579.1 50S ribosomal protein L21 -
  H7F36_RS10325 (H7F36_10325) rpmA 2185088..2185357 (+) 270 WP_187054580.1 50S ribosomal protein L27 -
  H7F36_RS10330 (H7F36_10330) cgtA 2185426..2186493 (+) 1068 WP_187054581.1 Obg family GTPase CgtA -
  H7F36_RS10335 (H7F36_10335) proB 2186499..2187674 (+) 1176 WP_187054900.1 glutamate 5-kinase -
  H7F36_RS10340 (H7F36_10340) - 2187675..2188112 (-) 438 WP_261802570.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62533.70 Da        Isoelectric Point: 5.6225

>NTDB_id=475543 H7F36_RS10305 WP_187054577.1 2181602..2183335(-) (pilB) [Variovorax sp. PAMC28562]
MAAAEPLVKENLPVALPGLARALISAGKLPAKTAEDIYQKSQSSRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDPQRLPKDLLDTKLCQAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMVEANAVSAAETI
DNIVGAEFEFDEVTADTSGDANEQAVAEVEDAPVVRFLHKMLLDAFSMRASDIHFEPYEHNYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGADRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKAR
LLQAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCIVCRTPAEIPR
EALLDAGFSATDADSGWTPYRPVGCSACNGGYKGRVGIYQVMPITEEIQKIILRDGSALDIARQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNQ

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=475543 H7F36_RS10305 WP_187054577.1 2181602..2183335(-) (pilB) [Variovorax sp. PAMC28562]
ATGGCCGCTGCCGAACCTCTCGTCAAAGAAAACCTGCCAGTTGCGCTGCCCGGTCTAGCGCGCGCTCTGATCTCCGCCGG
CAAATTGCCTGCCAAGACGGCTGAAGATATCTATCAAAAGTCGCAGTCGAGTCGCACCAGTTTCATTGCCGAATTGACCG
GCACAGGCGCAGTGTCGGCCGCCGACCTTGCCCACACGCTCTCCAGCGCTTTCGGCGCTCCGCTGCTAGACCTCGATGCA
ATCGACCCACAGCGTCTGCCCAAAGACCTGCTCGATACCAAGCTATGTCAGGCTTACCGCATCGTCGTTCTCAGCAAGCG
AAACAACCGACTCATCGTCGCTACCGCCGACCCGTCAGACCAGCAAGCCGCCGAGAAGATCAAGTTTGCGTCGCAAATGG
GCGTCGACTGGGTCATCGCCGAGTACGACAAGCTTTCGCGCATGGTCGAGGCCAATGCGGTCAGCGCGGCAGAAACCATC
GACAACATCGTTGGCGCCGAATTCGAGTTCGACGAAGTCACGGCCGACACATCGGGTGACGCCAATGAGCAGGCCGTCGC
CGAAGTCGAAGACGCGCCCGTGGTGCGCTTCCTGCACAAGATGCTGCTGGACGCCTTTAGCATGCGCGCGTCGGACATTC
ACTTCGAGCCGTACGAACACAACTACCGCGTTCGATTTCGCGTAGACGGTGAATTGCGCGAGATTGCGAGCCCGCCGACA
GTCATCAAAGACAAGCTGGCATCACGCATCAAGGTCATATCGCGGCTCGACATTTCTGAAAAGCGCGTGCCGCAAGACGG
TCGCATGAAGCTCAAGATCGGCGCCGACCGTGTCATCGATTTCCGGGTCAGCACGCTGCCGACCTTGTTCGGCGAAAAGA
TCGTTATCCGGATTCTCGATCCGAGCAGCGCCCGGCTGGGCATCGACGCACTGGGTTACGACGCCGACGAGAAGGCGCGT
TTGCTTCAGGCCATCGGTCGTCCTTACGGCATGGTTCTGGTGACCGGACCCACCGGCTCGGGAAAGACGGTGTCGCTCTA
CACCTGCCTGAATTTGCTTAACAAGCCGGGTGTGAATATCGCCACCGCGGAAGACCCGTCTGAGATCAACTTGCCGGGCG
TCAATCAGGTAAACGTCAACGAAAAGGCCGGACTGACCTTTGCGACGGCCCTGCGCGCCTTCTTGCGGCAGGACCCCGAC
ATCATCATGGTCGGCGAAATCCGGGATCTGGAGACTGCAGACATTTCCATCAAAGCGGCACAAACGGGCCACCTGGTGCT
GTCGACCCTTCACACCAACGACGCGCCGACCACGTTGACCCGCATGCGCAATATGGGGATTGCGCCTTTCAACATCGCAT
CGAGCGTCATCCTGATCACGGCGCAACGGCTGGCTCGGCGCCTTTGCATCGTGTGCCGGACGCCGGCCGAAATTCCGCGC
GAAGCATTGCTGGACGCCGGCTTTTCGGCGACGGACGCCGACAGCGGCTGGACGCCCTACCGCCCCGTGGGCTGTTCGGC
ATGCAACGGCGGCTACAAAGGCCGCGTCGGCATTTACCAGGTGATGCCGATCACCGAAGAAATTCAAAAAATCATCCTGC
GCGACGGCAGTGCGCTGGACATCGCTCGGCAGTCCGAGGCGGAGGGCGTTCGTTCGCTGCGTCAATCCGGCCTTCGGAAA
GTCATGCAGGGGCTGACATCACTCGAAGAAGTGGTCGCGTGCACCAACCAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8W597

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.417

98.094

0.534
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilF Neisseria gonorrhoeae MS11

52.669

97.4

0.513
  pilB Legionella pneumophila strain ERS1305867

48.485

97.227

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

46.046

98.614

0.454
  pilB Vibrio cholerae strain A1552

46.263

97.4

0.451
  pilB Vibrio campbellii strain DS40M4

45.907

97.4

0.447
  pilF Thermus thermophilus HB27

38.276

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.643

91.681

0.373