Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H6S61_RS06085 Genome accession   NZ_CP060047
Coordinates   1205264..1206952 (+) Length   562 a.a.
NCBI ID   WP_103187652.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1200264..1211952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS06065 (H6S61_06065) pdhR 1201867..1202634 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  H6S61_RS06070 (H6S61_06070) ampD 1203022..1203594 (-) 573 WP_080541072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H6S61_RS06075 (H6S61_06075) nadC 1203687..1204574 (+) 888 WP_130354580.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H6S61_RS06080 (H6S61_06080) pilA 1204797..1205264 (+) 468 WP_130270837.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  H6S61_RS06085 (H6S61_06085) pilB 1205264..1206952 (+) 1689 WP_103187652.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H6S61_RS06090 (H6S61_06090) pilC 1207000..1208226 (+) 1227 WP_130247867.1 type II secretion system F family protein Machinery gene
  H6S61_RS06095 (H6S61_06095) pilD 1208319..1209188 (+) 870 WP_038941424.1 A24 family peptidase Machinery gene
  H6S61_RS06100 (H6S61_06100) coaE 1209190..1209798 (+) 609 WP_045593985.1 dephospho-CoA kinase -
  H6S61_RS06105 (H6S61_06105) zapD 1209831..1210571 (+) 741 WP_017421189.1 cell division protein ZapD -
  H6S61_RS06110 (H6S61_06110) yacG 1210634..1210828 (+) 195 WP_038941426.1 DNA gyrase inhibitor YacG -
  H6S61_RS06115 (H6S61_06115) rplS 1211060..1211413 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62387.36 Da        Isoelectric Point: 5.5330

>NTDB_id=473533 H6S61_RS06085 WP_103187652.1 1205264..1206952(+) (pilB) [Vibrio vulnificus strain 2497-87]
MQSNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQDLQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=473533 H6S61_RS06085 WP_103187652.1 1205264..1206952(+) (pilB) [Vibrio vulnificus strain 2497-87]
ATGCAATCCAACCTTGCCACGATTCTTCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCCGATTGTCAAGATCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAACTGGCG
AATTTAGTCGAGGTTTCAGATGACGAAGTCGAGTCCATTGAAGACTTAAGCCAAGATGAATCTCCCGTTAGCCGCTTTAT
CAACCAAATTCTGCTGGATGCGGTACGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTAC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAA
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGCATTAAGCTACGCCTTAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACTTTACCCACGCTGTTTGGTGAAAAAATTGTGCTGCGACTTCTCGACAGCTCGAGTAGCA
GCTTGGATATCGACAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTATCTCTGTACACGGGCTTGAGTTTGCTCAACCGACCTGAAGT
GAATATCTCCACCGCGGAAGACCCGGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATAGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACCAACTCCGCTGCCGAAAC
CATTGTTCGCCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATCGCTCAGCGTCTTG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCGCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTAGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.335

100

0.763
  pilB Vibrio parahaemolyticus RIMD 2210633

75.801

100

0.758
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.341

92.883

0.477
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.608

99.466

0.404
  pilB/pilB1 Synechocystis sp. PCC 6803

37.063

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

94.306

0.365