Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H6S61_RS06080 Genome accession   NZ_CP060047
Coordinates   1204797..1205264 (+) Length   155 a.a.
NCBI ID   WP_130270837.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1199797..1210264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS06065 (H6S61_06065) pdhR 1201867..1202634 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  H6S61_RS06070 (H6S61_06070) ampD 1203022..1203594 (-) 573 WP_080541072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H6S61_RS06075 (H6S61_06075) nadC 1203687..1204574 (+) 888 WP_130354580.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H6S61_RS06080 (H6S61_06080) pilA 1204797..1205264 (+) 468 WP_130270837.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  H6S61_RS06085 (H6S61_06085) pilB 1205264..1206952 (+) 1689 WP_103187652.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H6S61_RS06090 (H6S61_06090) pilC 1207000..1208226 (+) 1227 WP_130247867.1 type II secretion system F family protein Machinery gene
  H6S61_RS06095 (H6S61_06095) pilD 1208319..1209188 (+) 870 WP_038941424.1 A24 family peptidase Machinery gene
  H6S61_RS06100 (H6S61_06100) coaE 1209190..1209798 (+) 609 WP_045593985.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16378.92 Da        Isoelectric Point: 8.7914

>NTDB_id=473532 H6S61_RS06080 WP_130270837.1 1204797..1205264(+) (pilA) [Vibrio vulnificus strain 2497-87]
MKKLHKTKKQQGFTLIELMIVVAIIGILAAFAIPAYQDYTKRATMAEFPRVASSTKLAVELCAHEHAADGAGFKSKCYLA
ANGIPAEFTLNNLKVTPTTGTASGSVDITVAATTDKGSIKTGEKYILTATYNATGITWEAVCKDAGGTPQTAYCP

Nucleotide


Download         Length: 468 bp        

>NTDB_id=473532 H6S61_RS06080 WP_130270837.1 1204797..1205264(+) (pilA) [Vibrio vulnificus strain 2497-87]
ATGAAGAAATTACACAAAACCAAGAAACAACAAGGTTTTACCTTGATTGAATTAATGATAGTGGTGGCGATTATTGGTAT
TTTGGCCGCCTTTGCTATTCCTGCTTATCAAGACTACACAAAGAGAGCTACTATGGCCGAGTTTCCGAGAGTAGCGTCTT
CAACAAAATTAGCAGTCGAACTTTGCGCTCACGAACATGCAGCAGATGGAGCTGGATTCAAGTCAAAATGCTACCTAGCA
GCAAATGGCATTCCTGCAGAGTTTACGTTAAACAATTTAAAAGTAACTCCAACCACAGGAACTGCATCAGGTTCCGTTGA
CATTACAGTAGCAGCAACAACAGATAAAGGCTCTATTAAGACCGGTGAAAAGTATATTCTTACCGCAACCTATAACGCTA
CTGGCATTACATGGGAAGCAGTTTGTAAAGATGCTGGTGGTACACCACAAACAGCCTACTGTCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

73.856

98.71

0.729
  pilA2 Legionella pneumophila strain ERS1305867

43.243

95.484

0.413
  pilA2 Legionella pneumophila str. Paris

42.568

95.484

0.406
  pilA Haemophilus influenzae 86-028NP

40.278

92.903

0.374
  pilA Glaesserella parasuis strain SC1401

38.095

94.839

0.361