Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R2866_RS01405 Genome accession   NC_017451
Coordinates   271421..272815 (+) Length   464 a.a.
NCBI ID   WP_005666685.1    Uniprot ID   -
Organism   Haemophilus influenzae R2866     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 266421..277815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2866_RS01380 (R2866_0276) rsmE 266439..267176 (-) 738 WP_005666674.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R2866_RS01385 (R2866_0277) lnt 267229..268755 (-) 1527 WP_020909963.1 apolipoprotein N-acyltransferase -
  R2866_RS01390 (R2866_0278) corC 268778..269677 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  R2866_RS01395 (R2866_0279) ampD 270309..270860 (-) 552 WP_005666680.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R2866_RS01400 (R2866_0280) pilA 270975..271424 (+) 450 WP_005666683.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  R2866_RS01405 (R2866_0281) pilB 271421..272815 (+) 1395 WP_005666685.1 GspE/PulE family protein Machinery gene
  R2866_RS01410 (R2866_0282) pilC 272812..274032 (+) 1221 WP_005666687.1 type II secretion system F family protein Machinery gene
  R2866_RS01415 (R2866_0283) pilD 274029..274721 (+) 693 WP_005666688.1 prepilin peptidase Machinery gene
  R2866_RS01420 (R2866_0284) rho 274776..276038 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  R2866_RS01425 (R2866_0285) metJ 276285..276602 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  R2866_RS01430 (R2866_0286) cueR 276616..277002 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  R2866_RS01435 (R2866_0287) - 277079..277285 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  R2866_RS01440 (R2866_0288) - 277360..277566 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52836.39 Da        Isoelectric Point: 6.1942

>NTDB_id=47258 R2866_RS01405 WP_005666685.1 271421..272815(+) (pilB) [Haemophilus influenzae R2866]
MTSYALLHTQHVTAKNGEVFTISPELWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQKLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIVQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIESSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKKS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=47258 R2866_RS01405 WP_005666685.1 271421..272815(+) (pilB) [Haemophilus influenzae R2866]
ATGACGAGCTATGCTTTACTTCATACTCAGCATGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGAGTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTTTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTAAAAGAACTGTTACAGAAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCCGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCTTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTTACAGGTCCCACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTGGATGGCATTGTTCAAAGCCAAATTAATCCACAGATTGGACTAGATTTTAACCGCCTATTGCGT
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTGCGTGC
TGCACAAACTGGGCATCTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATATCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAGCAGTTTACTGCTCGTCATTGCGCAGCGTCTTGTACGAAAAATCTGCCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGCTAGGTAAAAAGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(23-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

96.97

99.569

0.966
  pilB Haemophilus influenzae 86-028NP

96.336

100

0.963
  pilB Glaesserella parasuis strain SC1401

57.237

98.276

0.563
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.148

100

0.416
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.351

100

0.401
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.179

100

0.375
  pilF Thermus thermophilus HB27

35.714

100

0.366

Multiple sequence alignment