NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	H3M14_RS05110	Genome accession	NZ_CP059697
Coordinates	1018395..1019285 (+)	Length	296 a.a.
NCBI ID	WP_025016380.1	Uniprot ID	-
Organism	Latilactobacillus sakei strain CBA3635
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1013395..1024285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H3M14_RS05075 (H3M14_05075)	-	1013830..1014885 (+)	1056	WP_129584050.1	hypothetical protein	-
H3M14_RS05080 (H3M14_05080)	-	1014899..1015687 (+)	789	WP_061827022.1	hypothetical protein	-
H3M14_RS05085 (H3M14_05085)	-	1015684..1016298 (+)	615	WP_016265380.1	hypothetical protein	-
H3M14_RS05095 (H3M14_05095)	-	1016485..1016859 (-)	375	WP_011375004.1	hypothetical protein	-
H3M14_RS05100 (H3M14_05100)	-	1016956..1017456 (+)	501	WP_016265379.1	VanZ family protein	-
H3M14_RS05105 (H3M14_05105)	-	1017547..1018278 (+)	732	WP_011375002.1	YebC/PmpR family DNA-binding transcriptional regulator	-
H3M14_RS05110 (H3M14_05110)	comGA	1018395..1019285 (+)	891	WP_025016380.1	competence type IV pilus ATPase ComGA	Machinery gene
H3M14_RS05115 (H3M14_05115)	comGB	1019278..1020285 (+)	1008	WP_061827023.1	type II secretion system F family protein	Machinery gene
H3M14_RS05120 (H3M14_05120)	comGC	1020282..1020581 (+)	300	WP_025016383.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
H3M14_RS05125 (H3M14_05125)	comGD	1020601..1021005 (+)	405	WP_235596237.1	hypothetical protein	Machinery gene
H3M14_RS05130 (H3M14_05130)	comGE	1020992..1021297 (+)	306	WP_016265375.1	hypothetical protein	Machinery gene
H3M14_RS05135 (H3M14_05135)	comGF	1021272..1021784 (+)	513	WP_035145013.1	competence type IV pilus minor pilin ComGF	Machinery gene
H3M14_RS05140 (H3M14_05140)	-	1021747..1022085 (+)	339	WP_035145011.1	hypothetical protein	-
H3M14_RS05145 (H3M14_05145)	-	1022200..1023219 (+)	1020	WP_235596233.1	class I SAM-dependent methyltransferase	-

Sequence

Protein

Download Length: 296 a.a. Molecular weight: 32872.67 Da Isoelectric Point: 6.6910

                                >NTDB_id=471394 H3M14_RS05110 WP_025016380.1 1018395..1019285(+) (comGA) [Latilactobacillus sakei strain CBA3635]

MSTEEKLTTLLKHCAKHHVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL

QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA

KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTRG

VVARLLDLGISNLQLRACLTGIAYQSLVTKDDIVQAHYELLTGSDYFSAKEGGCDV

Nucleotide

Download Length: 891 bp

                                >NTDB_id=471394 H3M14_RS05110 WP_025016380.1 1018395..1019285(+) (comGA) [Latilactobacillus sakei strain CBA3635]

ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAGCACCATGTGCAAGATCTTTATTTTACACCGAC

AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC

GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA

CAACTGGCTGACCAAACAATTTATTTACGCCTAGCGACGGTTGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT

TATCTATCCAATTGGTGCCATTTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG

GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC

AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTCAACGATAATGCGGG

ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTGCGATTGCGTCCAGATACGCTGATTATCGGTGAGATTCGCGATTTAG

AGACGGCCCAATATGCCGTTTCCGCGGCTTTAAGTGGCCACCTGGTATTGAGCACAATCCATGCTCGAAGTACACGGGGC

GTGGTCGCGCGTTTATTGGATCTTGGTATTTCTAATCTGCAATTACGAGCCTGTCTAACGGGGATTGCCTATCAATCATT

GGTGACAAAAGACGACATTGTGCAAGCCCATTATGAGTTATTAACTGGTTCAGATTATTTCTCGGCAAAAGAAGGTGGAT

GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-269)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Latilactobacillus sakei subsp. sakei 23K	97.297	100	0.973
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	42.279	91.892	0.389
comGA/cglA/cilD	Streptococcus pneumoniae D39	41.912	91.892	0.385
comGA/cglA/cilD	Streptococcus pneumoniae R6	41.912	91.892	0.385
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	41.912	91.892	0.385
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	41.912	91.892	0.385
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	39.929	95.608	0.382
comGA	Lactococcus lactis subsp. cremoris KW2	37.884	98.986	0.375
comYA	Streptococcus gordonii str. Challis substr. CH1	40.755	89.527	0.365
comYA	Streptococcus mutans UA140	38.909	92.905	0.361
comYA	Streptococcus mutans UA159	38.909	92.905	0.361

Detailed information NTG0471394