Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HBNCFIEN_RS08240 Genome accession   NZ_CP059576
Coordinates   1933291..1934511 (-) Length   406 a.a.
NCBI ID   WP_182390639.1    Uniprot ID   A0A7G4RGG5
Organism   Legionella sp. PC997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1928291..1939511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBNCFIEN_RS08220 (HBNCFIEN_01658) - 1929164..1929718 (-) 555 WP_182390635.1 HPF/RaiA family ribosome-associated protein -
  HBNCFIEN_RS08225 (HBNCFIEN_01660) - 1930091..1931302 (-) 1212 WP_182390636.1 sterol desaturase family protein -
  HBNCFIEN_RS08230 (HBNCFIEN_01661) - 1931526..1932296 (-) 771 WP_182390637.1 HAD family hydrolase -
  HBNCFIEN_RS08235 (HBNCFIEN_01662) - 1932319..1933176 (-) 858 WP_182390638.1 A24 family peptidase -
  HBNCFIEN_RS08240 (HBNCFIEN_01663) pilC 1933291..1934511 (-) 1221 WP_182390639.1 type II secretion system F family protein Machinery gene
  HBNCFIEN_RS08245 (HBNCFIEN_01664) pilB 1934523..1936241 (-) 1719 WP_182390640.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBNCFIEN_RS08250 (HBNCFIEN_01665) - 1936497..1937264 (+) 768 WP_182390641.1 methyltransferase domain-containing protein -
  HBNCFIEN_RS08255 (HBNCFIEN_01666) - 1937271..1937837 (-) 567 WP_182390642.1 hypothetical protein -
  HBNCFIEN_RS08260 (HBNCFIEN_01667) - 1938019..1938345 (+) 327 WP_182390643.1 DUF1820 family protein -
  HBNCFIEN_RS08265 (HBNCFIEN_01668) - 1938388..1938948 (+) 561 WP_182390644.1 hypoxanthine-guanine phosphoribosyltransferase -
  HBNCFIEN_RS08270 (HBNCFIEN_01669) - 1939209..1939394 (+) 186 WP_182390645.1 hypothetical protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44410.41 Da        Isoelectric Point: 9.9034

>NTDB_id=470909 HBNCFIEN_RS08240 WP_182390639.1 1933291..1934511(-) (pilC) [Legionella sp. PC997]
MKKNANTTLTFHYTGVNKAAQQVNGDIEARSLALAKVELRRQGIVVNKIAKKRPPLFSLKSKKIKSGDITVFSRQLATMI
ESGIPLVQSFDIVAKGQSNKRFKELIETIKRDVETGLTLSESLQKFPAYFNELFCNLVEAGEKSGSLDIMLDKVATYKEK
IETIKKKIKKALTYPLAVMVVAFLVTAGLLIFVVPQFEALFKGFGADLPAMTQAVVSMSKIFQSYWYLIFGVLGGAIYAF
VYAKKHSPQFAQNVDRMLLKIPVIGPIIEKAAIARFARTLSITFAAGLPLVEALKSVAGATGNIIFAKGTDNIREEVSTG
QQMNKAMENTQLFPNMVIQMVAIGEESGALEKMLSKVADFYEEDVDNAVDSLSSLLEPVIMCVLGVLVGGLVVAMYLPIF
KLGSAI

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=470909 HBNCFIEN_RS08240 WP_182390639.1 1933291..1934511(-) (pilC) [Legionella sp. PC997]
ATGAAAAAAAATGCCAATACAACCTTAACTTTTCATTACACTGGAGTTAATAAGGCAGCTCAACAAGTAAATGGAGATAT
TGAGGCCAGAAGTCTTGCTTTAGCCAAAGTCGAATTACGCCGACAAGGAATTGTTGTCAATAAAATTGCTAAAAAACGTC
CACCTCTATTTAGTTTAAAAAGTAAAAAAATAAAATCTGGGGATATAACTGTATTTAGTCGCCAATTGGCCACAATGATT
GAATCCGGGATCCCTCTAGTACAATCCTTTGATATTGTGGCAAAAGGACAATCCAATAAACGATTTAAAGAATTAATTGA
GACAATAAAAAGAGATGTTGAGACAGGATTAACGCTCTCAGAATCACTGCAAAAGTTTCCAGCATATTTTAATGAATTAT
TTTGTAATTTGGTTGAGGCGGGTGAGAAATCGGGATCTCTAGATATTATGCTGGATAAAGTTGCAACCTATAAAGAAAAA
ATAGAGACAATAAAGAAGAAAATTAAAAAGGCATTAACCTACCCTCTCGCGGTAATGGTTGTTGCTTTCCTTGTTACTGC
AGGTTTACTTATTTTTGTGGTTCCACAATTTGAGGCATTATTTAAAGGTTTCGGTGCCGACTTACCTGCAATGACCCAAG
CTGTAGTGAGTATGTCGAAAATTTTTCAATCCTATTGGTATCTAATTTTTGGAGTTTTAGGAGGCGCGATTTATGCATTT
GTTTATGCGAAAAAACACTCACCCCAATTCGCGCAAAATGTAGATAGAATGCTGTTAAAAATACCCGTAATTGGTCCTAT
TATTGAAAAAGCAGCGATCGCTCGTTTTGCAAGAACTTTATCGATTACGTTCGCAGCCGGCTTGCCTTTAGTTGAAGCGC
TTAAATCTGTGGCAGGTGCCACAGGAAATATTATTTTTGCCAAGGGTACTGATAATATTAGGGAAGAGGTCTCCACGGGA
CAGCAAATGAATAAAGCTATGGAGAATACGCAATTATTTCCCAACATGGTGATCCAGATGGTTGCCATTGGTGAGGAATC
AGGTGCTTTAGAAAAAATGTTAAGTAAAGTTGCTGATTTTTATGAAGAAGATGTTGATAATGCAGTTGACTCCCTGAGTA
GTTTATTAGAGCCTGTTATTATGTGTGTTTTAGGTGTCTTGGTCGGTGGACTGGTTGTTGCAATGTATCTACCTATCTTT
AAACTGGGATCTGCAATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G4RGG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

80.788

100

0.808
  pilC Acinetobacter baumannii D1279779

53.827

99.754

0.537
  pilC Acinetobacter baylyi ADP1

53.904

97.783

0.527
  pilC Pseudomonas stutzeri DSM 10701

52.709

100

0.527
  pilG Neisseria meningitidis 44/76-A

44.31

100

0.451
  pilG Neisseria gonorrhoeae MS11

44.068

100

0.448
  pilC Vibrio cholerae strain A1552

41.919

97.537

0.409
  pilC Vibrio campbellii strain DS40M4

38.049

100

0.384
  pilC Thermus thermophilus HB27

36.908

98.768

0.365