Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HBNCFIEN_RS08245 Genome accession   NZ_CP059576
Coordinates   1934523..1936241 (-) Length   572 a.a.
NCBI ID   WP_182390640.1    Uniprot ID   A0A7G4RGG6
Organism   Legionella sp. PC997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1929523..1941241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBNCFIEN_RS08225 (HBNCFIEN_01660) - 1930091..1931302 (-) 1212 WP_182390636.1 sterol desaturase family protein -
  HBNCFIEN_RS08230 (HBNCFIEN_01661) - 1931526..1932296 (-) 771 WP_182390637.1 HAD family hydrolase -
  HBNCFIEN_RS08235 (HBNCFIEN_01662) - 1932319..1933176 (-) 858 WP_182390638.1 A24 family peptidase -
  HBNCFIEN_RS08240 (HBNCFIEN_01663) pilC 1933291..1934511 (-) 1221 WP_182390639.1 type II secretion system F family protein Machinery gene
  HBNCFIEN_RS08245 (HBNCFIEN_01664) pilB 1934523..1936241 (-) 1719 WP_182390640.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBNCFIEN_RS08250 (HBNCFIEN_01665) - 1936497..1937264 (+) 768 WP_182390641.1 methyltransferase domain-containing protein -
  HBNCFIEN_RS08255 (HBNCFIEN_01666) - 1937271..1937837 (-) 567 WP_182390642.1 hypothetical protein -
  HBNCFIEN_RS08260 (HBNCFIEN_01667) - 1938019..1938345 (+) 327 WP_182390643.1 DUF1820 family protein -
  HBNCFIEN_RS08265 (HBNCFIEN_01668) - 1938388..1938948 (+) 561 WP_182390644.1 hypoxanthine-guanine phosphoribosyltransferase -
  HBNCFIEN_RS08270 (HBNCFIEN_01669) - 1939209..1939394 (+) 186 WP_182390645.1 hypothetical protein -
  HBNCFIEN_RS08275 (HBNCFIEN_01670) - 1939939..1940580 (-) 642 WP_182390646.1 hypothetical protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62912.63 Da        Isoelectric Point: 6.3298

>NTDB_id=470910 HBNCFIEN_RS08245 WP_182390640.1 1934523..1936241(-) (pilB) [Legionella sp. PC997]
MLGSDEFKLHGIGQLFVLEKLLDKPQALEFCKNASAEKISLVQYLVKNNILSATQIALTAAHNFGAPMLDLDCIDIDSIP
TDLVNEKLIRRHSMVPLFCRGNNLYLATEDPSKQASLKEIQFHTGLNTHAIIVEADKLSALIDHLLTVKETQGLSEFVGD
TSEFDGIIINEEEEHETAVVTSLTEDAPIVKFVNKILLDAIKQGASDIHFEPYEKEYRIRYRQDGILHEIATPPGSLSTR
ITARIKIMSSLDISERRVPQDGGFKMKISKTRSIDFRVSTCPTTSGEKVVMRILDPGSAKLGIEALGFSPIQKEYFKHAI
ERPQGMILVTGPTGSGKTVTLYTALNILNTKEVNISTAEDPVEIKVAGINQVNINPKAGLTFSNALRSFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIATFNVASSVTLIIAQRLARKLCEHCKVLRDDFNKPSLL
ELGFKESDLEGIKLYKAAGCARCTSGYRGRIGLFEVLPMTKKLGQIIMAGGNSLEILKIAQEEGMITVYQSGLEKIKQGI
TTIEEVNRVTVD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=470910 HBNCFIEN_RS08245 WP_182390640.1 1934523..1936241(-) (pilB) [Legionella sp. PC997]
ATGTTAGGAAGCGATGAATTTAAATTACATGGAATAGGACAATTGTTTGTTCTTGAAAAGCTTTTGGACAAACCACAAGC
TCTTGAGTTCTGTAAAAATGCCTCTGCAGAAAAAATATCTTTAGTTCAATACCTTGTCAAAAATAACATCTTATCAGCCA
CCCAAATTGCACTTACAGCAGCACATAATTTTGGTGCTCCTATGCTGGACCTAGACTGTATTGACATAGATTCTATTCCT
ACTGATCTGGTGAATGAAAAGTTAATTCGTCGTCATTCAATGGTTCCTCTATTTTGTCGCGGAAATAACTTATATCTTGC
AACTGAAGATCCAAGCAAACAAGCTTCTTTAAAGGAGATTCAATTTCATACGGGACTTAATACACATGCAATTATCGTTG
AGGCAGATAAGCTTAGTGCATTAATTGATCATTTACTCACCGTTAAGGAAACTCAAGGATTATCTGAATTTGTGGGTGAT
ACAAGTGAGTTCGATGGAATTATCATCAATGAAGAAGAAGAGCATGAGACAGCAGTTGTTACTTCGCTAACGGAGGATGC
TCCTATTGTTAAATTCGTTAACAAAATACTTCTAGATGCCATCAAACAAGGAGCTTCTGACATCCATTTTGAACCCTATG
AAAAAGAGTACCGCATACGCTATCGACAAGACGGTATTTTACATGAAATTGCCACCCCACCTGGAAGCTTATCCACACGA
ATTACTGCACGTATAAAAATCATGTCGAGTCTTGATATTTCGGAAAGACGAGTTCCTCAGGATGGCGGCTTTAAAATGAA
AATATCCAAAACGCGATCCATTGATTTTCGTGTAAGCACCTGCCCTACTACCTCAGGTGAAAAAGTTGTTATGCGGATCT
TAGACCCTGGTTCTGCAAAATTAGGTATTGAAGCTTTAGGTTTTAGCCCTATTCAAAAAGAATATTTTAAACATGCTATT
GAACGACCACAGGGAATGATCCTGGTAACAGGTCCAACAGGTAGTGGTAAAACCGTAACGTTGTATACCGCATTAAATAT
TCTTAACACTAAAGAAGTCAACATTTCTACGGCTGAAGATCCCGTAGAAATTAAAGTAGCTGGCATTAATCAAGTTAATA
TTAATCCTAAAGCGGGATTAACTTTTTCTAATGCATTACGTTCTTTTTTACGACAAGATCCTGATATCATCATGGTTGGT
GAGATACGTGATTTAGAAACGGCGGAGATTGCAGTTAAAGCAGCTCAAACAGGACATTTAGTCCTCTCGACCTTACATAC
GAATAGTGCCGCTGAAACATTAAATCGTCTAGTTAATATGGGAATTGCAACATTTAATGTGGCCAGCTCCGTAACACTTA
TTATTGCCCAGCGTTTAGCTCGAAAATTATGCGAACATTGTAAAGTCTTAAGAGATGACTTCAATAAACCGAGTTTGTTA
GAGTTAGGTTTTAAAGAGTCTGATTTGGAAGGAATTAAGTTATATAAAGCTGCAGGATGCGCTAGGTGTACCAGCGGATA
CCGTGGAAGGATTGGATTATTTGAGGTGCTGCCTATGACTAAAAAACTTGGCCAGATCATCATGGCTGGAGGAAACTCTC
TGGAAATATTAAAAATTGCCCAGGAAGAAGGGATGATTACGGTTTATCAATCAGGACTTGAAAAGATAAAACAAGGGATT
ACAACTATTGAGGAAGTCAATCGGGTAACCGTTGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-146)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G4RGG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

79.545

100

0.795
  pilB Acinetobacter baylyi ADP1

53.694

97.028

0.521
  pilB Acinetobacter baumannii D1279779

53.65

95.804

0.514
  pilB Vibrio cholerae strain A1552

48.561

97.203

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

47.957

98.427

0.472
  pilB Vibrio campbellii strain DS40M4

48.885

94.056

0.46
  pilF Neisseria gonorrhoeae MS11

46.195

98.776

0.456
  pilF Thermus thermophilus HB27

41.527

93.881

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.643

92.483

0.376