Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   H3L94_RS01485 Genome accession   NZ_CP059567
Coordinates   322065..323309 (+) Length   414 a.a.
NCBI ID   WP_182122376.1    Uniprot ID   -
Organism   Neisseria shayeganii strain DSM 22244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 317065..328309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L94_RS12240 - 317340..317542 (+) 203 Protein_295 ATP-dependent exonuclease -
  H3L94_RS01470 (H3L94_01470) glcF 317552..318787 (-) 1236 WP_182122373.1 glycolate oxidase subunit GlcF -
  H3L94_RS01475 (H3L94_01475) glcE 318861..319937 (-) 1077 WP_182122374.1 glycolate oxidase subunit GlcE -
  H3L94_RS01480 (H3L94_01480) pilF 320356..322032 (+) 1677 WP_182122375.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L94_RS01485 (H3L94_01485) pilG 322065..323309 (+) 1245 WP_182122376.1 type II secretion system F family protein Machinery gene
  H3L94_RS01490 (H3L94_01490) pilD 323483..324337 (+) 855 WP_182122377.1 A24 family peptidase Machinery gene
  H3L94_RS01495 (H3L94_01495) coaE 324338..324946 (+) 609 WP_182122378.1 dephospho-CoA kinase -
  H3L94_RS01500 (H3L94_01500) zapD 324950..325726 (+) 777 WP_182122379.1 cell division protein ZapD -
  H3L94_RS01505 (H3L94_01505) yacG 325723..325944 (+) 222 WP_182122380.1 DNA gyrase inhibitor YacG -
  H3L94_RS01510 (H3L94_01510) rlmD 326036..327328 (+) 1293 WP_182122381.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Sequence

Protein


Download         Length: 414 a.a.        Molecular weight: 45579.72 Da        Isoelectric Point: 9.6006

>NTDB_id=470767 H3L94_RS01485 WP_182122376.1 322065..323309(+) (pilG) [Neisseria shayeganii strain DSM 22244]
MVLNRKTSVNQAAAKQTGNRFEFEGRSLDTEQMVRGEVVAKDEKEARAKLARRRIKVIHIHKKKKVRQKKITQADITVFT
RQLATMMKAGLPLMQAFDIVAKGHSNASMTKLLMEIRSDIEQGTSMADAFRKHPKYFDSFYCNLVEAGEAGGVLENLLDK
LAVYKEKTQAIKKKIKSALTYPIAVVVVAIVLIIIMMMFVLPEFKKVYDGMNAEMPGLTQFMMGISDFMVEYGWLVIIGL
IGLVAGFVQWHKHSPAMQKRVDALLLKLPIFGNIVEKGTIARWARTTATLFTAGVPLVEALDSVAGASGNIIYEEATKGI
RSQVNQGSSLTSAMNATDLFPNMVVQMTSIGEESGALDDMLNKAAEFYEDEVDMAVATLSSLMEPIIMVVLGSIIGVILV
AMYLPLFNLGSVVG

Nucleotide


Download         Length: 1245 bp        

>NTDB_id=470767 H3L94_RS01485 WP_182122376.1 322065..323309(+) (pilG) [Neisseria shayeganii strain DSM 22244]
ATGGTTCTAAACAGGAAAACGTCTGTCAATCAAGCTGCAGCCAAACAAACCGGCAACCGTTTCGAATTTGAAGGCCGCAG
TTTGGATACCGAGCAAATGGTACGCGGCGAAGTAGTGGCAAAAGATGAGAAAGAGGCGCGCGCCAAGCTGGCCCGCCGCC
GCATCAAAGTCATCCACATTCATAAAAAGAAAAAAGTCCGCCAGAAGAAAATTACCCAAGCTGACATCACGGTGTTTACC
CGCCAATTGGCCACCATGATGAAGGCCGGTTTGCCTTTGATGCAGGCATTCGATATTGTGGCCAAAGGACATTCCAACGC
CTCGATGACCAAACTGCTGATGGAAATCCGCAGCGATATTGAGCAAGGTACCTCTATGGCCGATGCGTTCCGGAAACACC
CGAAATACTTCGATTCGTTTTACTGCAACCTGGTAGAGGCCGGTGAGGCGGGCGGCGTGTTGGAAAATCTGCTGGATAAA
TTGGCTGTATACAAAGAGAAAACCCAAGCGATTAAAAAGAAAATCAAGAGTGCACTCACTTATCCGATTGCTGTAGTAGT
GGTGGCCATTGTTTTGATCATCATCATGATGATGTTTGTTTTGCCGGAATTTAAGAAGGTATATGACGGCATGAATGCGG
AAATGCCCGGACTGACCCAATTTATGATGGGGATTTCGGATTTCATGGTCGAATACGGTTGGTTGGTGATCATCGGCTTG
ATTGGTCTTGTCGCCGGCTTTGTTCAATGGCACAAACATTCGCCTGCCATGCAAAAGCGGGTGGATGCGCTGCTGTTGAA
ACTGCCGATTTTCGGCAATATTGTCGAAAAAGGTACCATTGCCCGCTGGGCGCGGACAACGGCAACTTTATTTACCGCCG
GTGTGCCTTTGGTGGAGGCTTTGGACTCGGTTGCGGGCGCCTCGGGCAATATTATTTATGAAGAAGCGACCAAAGGCATT
CGCAGTCAAGTCAATCAAGGCAGTTCTTTAACTTCGGCCATGAATGCAACCGATCTGTTTCCCAATATGGTGGTACAGAT
GACATCTATTGGGGAGGAATCGGGTGCTTTAGACGATATGCTGAACAAAGCGGCTGAATTCTACGAAGATGAGGTGGATA
TGGCGGTGGCCACGCTGTCATCGCTGATGGAGCCCATTATCATGGTGGTGTTGGGCAGCATTATTGGGGTGATTTTGGTG
GCCATGTATCTGCCCTTGTTCAATTTGGGTAGTGTGGTCGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-202)

Type II secretion system (T2SS), protein F (T2SSF)

(283-405)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

66.244

95.169

0.63
  pilG Neisseria meningitidis 44/76-A

65.482

95.169

0.623
  pilC Legionella pneumophila strain ERS1305867

50.126

95.894

0.481
  pilC Acinetobacter baumannii D1279779

47.118

96.377

0.454
  pilC Pseudomonas stutzeri DSM 10701

47.355

95.894

0.454
  pilC Acinetobacter baylyi ADP1

46.5

96.618

0.449
  pilC Vibrio cholerae strain A1552

39.481

92.995

0.367
  pilC Vibrio campbellii strain DS40M4

38.718

94.203

0.365