Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H3L94_RS01480 Genome accession   NZ_CP059567
Coordinates   320356..322032 (+) Length   558 a.a.
NCBI ID   WP_182122375.1    Uniprot ID   A0A7D7N5P2
Organism   Neisseria shayeganii strain DSM 22244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315356..327032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L94_RS12240 - 317340..317542 (+) 203 Protein_295 ATP-dependent exonuclease -
  H3L94_RS01470 (H3L94_01470) glcF 317552..318787 (-) 1236 WP_182122373.1 glycolate oxidase subunit GlcF -
  H3L94_RS01475 (H3L94_01475) glcE 318861..319937 (-) 1077 WP_182122374.1 glycolate oxidase subunit GlcE -
  H3L94_RS01480 (H3L94_01480) pilF 320356..322032 (+) 1677 WP_182122375.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L94_RS01485 (H3L94_01485) pilG 322065..323309 (+) 1245 WP_182122376.1 type II secretion system F family protein Machinery gene
  H3L94_RS01490 (H3L94_01490) pilD 323483..324337 (+) 855 WP_182122377.1 A24 family peptidase Machinery gene
  H3L94_RS01495 (H3L94_01495) coaE 324338..324946 (+) 609 WP_182122378.1 dephospho-CoA kinase -
  H3L94_RS01500 (H3L94_01500) zapD 324950..325726 (+) 777 WP_182122379.1 cell division protein ZapD -
  H3L94_RS01505 (H3L94_01505) yacG 325723..325944 (+) 222 WP_182122380.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62401.14 Da        Isoelectric Point: 5.4378

>NTDB_id=470766 H3L94_RS01480 WP_182122375.1 320356..322032(+) (pilF) [Neisseria shayeganii strain DSM 22244]
MSVGLLRILFQNHLLTQEQAEKYREKVNQNKNIIPMLAEAGIIRQQELAALLAKLFNYPMMDLSHYSRSLMVPDILDDEQ
MAEYRCVPLFKRGRRLFLGVSDPTLLQTYQKLVFPSGLIVDLVILNDEQLDSLLELVRQTSTALLEGIAAEQQFSGEGAK
NMLLEDEEEDGPIAKFIHKVLADALNGGASDIHFEFYEEMARIRFRTDGQLREVVQPPLNIRGQIASRIKVMAKLDISEK
RIPQDGRIQIQFNKNQRPIDFRVNTLPTLFGEKIVMRILNSDAATLNINQLGFEPFQKELLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTESVNISTAEDPAEINLPGINQVNVNDKQGMTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKQPIERPPTPALLKAGFTEEDLQGDWQMY
RQVGCDRCKGKGFKGRAGVYEIMPVSEAMQKIIMNNGTEVDIAEQAYRDGLVDLRRAGLLKVMQGVTTLEEILASTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=470766 H3L94_RS01480 WP_182122375.1 320356..322032(+) (pilF) [Neisseria shayeganii strain DSM 22244]
ATGAGTGTCGGACTGCTGCGCATCTTGTTTCAAAATCACTTGCTTACTCAGGAACAGGCTGAAAAATACCGCGAAAAAGT
AAACCAAAACAAAAACATTATTCCGATGCTGGCGGAGGCCGGCATCATCCGACAGCAGGAGTTGGCCGCTCTGCTGGCCA
AACTGTTCAATTATCCGATGATGGATTTGTCGCATTATTCGCGTTCGCTGATGGTGCCGGATATCTTGGATGACGAGCAG
ATGGCGGAATACCGCTGCGTGCCGCTGTTTAAACGCGGGCGGCGGCTGTTTTTAGGCGTTTCCGATCCGACACTGTTGCA
AACCTACCAAAAATTGGTTTTCCCCAGTGGCCTGATTGTGGACTTGGTCATTTTGAATGACGAGCAATTGGACAGCTTGC
TGGAACTGGTGCGCCAAACCTCTACCGCCTTGTTGGAAGGTATTGCCGCAGAGCAGCAATTCAGTGGCGAGGGCGCCAAA
AATATGCTGCTGGAGGACGAGGAAGAAGACGGCCCGATTGCCAAATTTATCCATAAAGTATTGGCCGATGCGCTCAACGG
CGGTGCGTCGGACATTCATTTCGAGTTTTATGAGGAAATGGCGCGTATCCGTTTCCGTACCGACGGCCAGCTGCGCGAGG
TGGTGCAGCCGCCGCTGAATATCCGCGGCCAAATCGCTTCGCGTATCAAAGTCATGGCCAAGCTCGATATTTCCGAAAAG
CGGATCCCGCAAGACGGCCGTATTCAGATTCAGTTCAATAAAAATCAGCGGCCGATTGATTTCCGGGTCAATACCTTGCC
CACCCTGTTTGGCGAAAAGATTGTGATGCGTATCCTGAATTCGGATGCGGCGACGCTGAACATCAACCAGCTGGGTTTTG
AGCCATTCCAAAAAGAGCTGCTGCTTGAAGCCATCCACCGGCCTTACGGCATGGTGTTGGTTACCGGGCCGACGGGTTCG
GGTAAAACTGTTTCGCTTTATACCTGCCTGAATATTTTGAATACCGAGAGCGTGAATATCTCCACGGCGGAAGATCCGGC
GGAGATTAATCTGCCCGGCATCAACCAGGTAAACGTAAACGACAAACAAGGCATGACTTTTGCTGCTGCTTTGAAGGCGT
TTCTGCGCCAAGACCCGGACATCATCATGGTGGGTGAGATTCGGGACTTGGAAACGGCCGATATTGCCATTAAAGCGGCC
CAAACCGGCCACATGGTGTTTTCAACCCTGCATACCAATAATGCGCCGGCTACCTTATCCAGGATGTTGAACATGGGCGT
GGCGCCGTTCAATATAGCCAGTTCAGTCAATCTGATTATGGCGCAGCGCTTGGTACGCCGTTTGTGTACCTGCAAGCAGC
CAATTGAGCGCCCGCCTACGCCGGCTTTGTTGAAAGCCGGATTTACCGAAGAAGATTTGCAGGGTGATTGGCAGATGTAC
CGCCAAGTGGGTTGCGACCGCTGCAAAGGCAAAGGTTTCAAGGGTCGGGCCGGTGTGTATGAAATCATGCCGGTGAGCGA
AGCCATGCAAAAAATCATCATGAATAACGGCACCGAAGTGGACATTGCCGAACAGGCTTACCGTGACGGTTTGGTTGATT
TGCGACGTGCCGGCTTATTGAAGGTGATGCAGGGGGTGACCACTTTGGAGGAAATCCTCGCCAGCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-137)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D7N5P2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

70

100

0.703
  pilB Acinetobacter baylyi ADP1

50.176

100

0.511
  pilB Acinetobacter baumannii D1279779

53.592

92.294

0.495
  pilB Vibrio cholerae strain A1552

46.032

100

0.468
  pilB Legionella pneumophila strain ERS1305867

45.343

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

43.816

100

0.444
  pilB Vibrio campbellii strain DS40M4

43.182

100

0.443
  pilF Thermus thermophilus HB27

39.962

94.624

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.223

92.294

0.362