Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   H3L98_RS05215 Genome accession   NZ_CP059563
Coordinates   939318..940586 (-) Length   422 a.a.
NCBI ID   WP_027021066.1    Uniprot ID   A0AAE9KYV5
Organism   Conchiformibius steedae strain DSM 2580     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 934318..945586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L98_RS05190 (H3L98_05195) - 934764..935708 (+) 945 WP_027021062.1 LysR substrate-binding domain-containing protein -
  H3L98_RS05195 (H3L98_05200) - 935739..936974 (+) 1236 WP_169733441.1 YihY family inner membrane protein -
  H3L98_RS05200 (H3L98_05205) zapD 937019..937768 (-) 750 WP_027021063.1 cell division protein ZapD -
  H3L98_RS05205 (H3L98_05210) coaE 937793..938398 (-) 606 WP_027021064.1 dephospho-CoA kinase -
  H3L98_RS05210 (H3L98_05215) pilD 938402..939313 (-) 912 WP_027021065.1 prepilin peptidase Machinery gene
  H3L98_RS05215 (H3L98_05220) pilG 939318..940586 (-) 1269 WP_027021066.1 type II secretion system F family protein Machinery gene
  H3L98_RS05220 (H3L98_05225) pilF 940675..942354 (-) 1680 WP_027021067.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L98_RS05225 (H3L98_05230) mtgA 942482..943168 (-) 687 WP_027021068.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  H3L98_RS05230 (H3L98_05235) aroE 943306..944106 (-) 801 Protein_915 shikimate dehydrogenase -
  H3L98_RS05235 (H3L98_05240) hisC 944394..945455 (+) 1062 WP_027021069.1 histidinol-phosphate transaminase -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46332.78 Da        Isoelectric Point: 10.0989

>NTDB_id=470649 H3L98_RS05215 WP_027021066.1 939318..940586(-) (pilG) [Conchiformibius steedae strain DSM 2580]
MAAKKPVKTVPAKKAAAFSPKKKEKGNRYQFEGRNLQTESIVRGEVVARNEEEARQKLQRRQVKVLQITKMKKTRDKKIT
QADLTVFTRQLSTMMKAGLPLMQAFDIVAKGHSNATMTRMLLSVRADVEQGTSLASAFAKHPKYFDKFYCNLVAAGEAGG
VLEALLDKLATYKEKTEAIKKKVKSALTYPISVIVVAIVLVIVMMMFVLPEFKKVYDGLGAELPALTAFMMNISDIFVAY
GWLIILGLVGFVVALVQIHQRSPAFQKKVDAWLLKMPIFGQIVQKATIARWARTTATLFAAGVPLVEALDSVAGASGNIL
YEEATHEIRSKVNQGISLTSSMQAAQIFPNMVVQMASIGEESGALDDMLNKVAEFYEDEVDNAVAMLSSLMEPIIMVVLG
SIIGVILVAMYLPLFNIGNVFG

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=470649 H3L98_RS05215 WP_027021066.1 939318..940586(-) (pilG) [Conchiformibius steedae strain DSM 2580]
ATGGCCGCCAAAAAACCCGTCAAAACCGTCCCAGCGAAAAAAGCCGCAGCCTTTTCGCCCAAAAAGAAAGAAAAAGGCAA
CCGCTACCAGTTTGAAGGGCGCAACCTGCAAACCGAATCCATTGTGCGCGGTGAAGTGGTTGCCCGCAACGAAGAAGAAG
CCCGCCAAAAGCTGCAGCGCCGTCAGGTAAAGGTGCTGCAAATCACCAAAATGAAAAAAACGCGGGACAAAAAAATCACG
CAGGCAGATTTAACCGTGTTTACCCGCCAGCTTTCCACCATGATGAAAGCTGGTTTGCCGCTGATGCAGGCATTTGATAT
TGTGGCAAAAGGGCATTCCAACGCCACCATGACGCGCATGTTGTTAAGTGTGCGTGCCGACGTGGAACAAGGTACGTCTT
TGGCAAGCGCGTTTGCCAAACACCCCAAATACTTTGACAAATTCTATTGCAACTTGGTGGCGGCAGGTGAAGCGGGCGGT
GTATTGGAAGCCCTGCTCGACAAACTCGCCACTTATAAAGAAAAAACCGAAGCCATCAAGAAAAAAGTAAAAAGCGCATT
GACTTACCCGATTTCGGTTATCGTGGTGGCGATTGTGCTGGTGATTGTGATGATGATGTTCGTGTTGCCCGAATTTAAAA
AGGTATATGACGGCTTGGGCGCAGAACTGCCCGCGCTGACTGCCTTTATGATGAATATTTCCGATATATTCGTGGCTTAC
GGCTGGTTGATTATTTTGGGCTTGGTGGGCTTTGTTGTCGCGCTGGTGCAAATTCACCAACGCTCGCCCGCTTTCCAGAA
AAAAGTAGATGCATGGCTGCTGAAAATGCCGATTTTCGGTCAGATTGTGCAAAAAGCCACCATTGCCCGTTGGGCGCGTA
CCACTGCCACGCTGTTTGCAGCGGGTGTGCCATTGGTGGAAGCCTTGGATTCGGTGGCGGGTGCATCGGGCAATATTTTG
TATGAAGAAGCCACACACGAAATCCGCAGCAAGGTGAATCAGGGTATTTCACTGACTTCATCTATGCAAGCAGCGCAGAT
TTTCCCCAATATGGTGGTACAAATGGCATCTATTGGTGAAGAATCGGGTGCTTTGGACGATATGCTGAACAAAGTGGCGG
AATTTTATGAAGACGAAGTGGATAATGCTGTGGCGATGCTGTCGTCTTTGATGGAACCGATTATTATGGTGGTATTGGGT
AGTATTATCGGTGTGATTTTGGTGGCAATGTATCTGCCGCTGTTTAATATCGGTAACGTATTCGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

66.165

94.55

0.626
  pilG Neisseria meningitidis 44/76-A

65.915

94.55

0.623
  pilC Legionella pneumophila strain ERS1305867

48.485

93.839

0.455
  pilC Pseudomonas stutzeri DSM 10701

45.906

95.498

0.438
  pilC Acinetobacter baumannii D1279779

45.679

95.972

0.438
  pilC Acinetobacter baylyi ADP1

45.974

91.232

0.419
  pilC Vibrio cholerae strain A1552

38.903

95.024

0.37
  pilC Vibrio campbellii strain DS40M4

39.196

94.313

0.37