Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H3L98_RS05220 Genome accession   NZ_CP059563
Coordinates   940675..942354 (-) Length   559 a.a.
NCBI ID   WP_027021067.1    Uniprot ID   A0AAE9KZL2
Organism   Conchiformibius steedae strain DSM 2580     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 935675..947354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L98_RS05195 (H3L98_05200) - 935739..936974 (+) 1236 WP_169733441.1 YihY family inner membrane protein -
  H3L98_RS05200 (H3L98_05205) zapD 937019..937768 (-) 750 WP_027021063.1 cell division protein ZapD -
  H3L98_RS05205 (H3L98_05210) coaE 937793..938398 (-) 606 WP_027021064.1 dephospho-CoA kinase -
  H3L98_RS05210 (H3L98_05215) pilD 938402..939313 (-) 912 WP_027021065.1 prepilin peptidase Machinery gene
  H3L98_RS05215 (H3L98_05220) pilG 939318..940586 (-) 1269 WP_027021066.1 type II secretion system F family protein Machinery gene
  H3L98_RS05220 (H3L98_05225) pilF 940675..942354 (-) 1680 WP_027021067.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L98_RS05225 (H3L98_05230) mtgA 942482..943168 (-) 687 WP_027021068.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  H3L98_RS05230 (H3L98_05235) aroE 943306..944106 (-) 801 Protein_915 shikimate dehydrogenase -
  H3L98_RS05235 (H3L98_05240) hisC 944394..945455 (+) 1062 WP_027021069.1 histidinol-phosphate transaminase -
  H3L98_RS05240 (H3L98_05245) - 945443..946267 (-) 825 WP_027021070.1 hypothetical protein -
  H3L98_RS05245 (H3L98_05250) hisB 946367..946957 (+) 591 Protein_918 imidazoleglycerol-phosphate dehydratase HisB -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62241.67 Da        Isoelectric Point: 5.3064

>NTDB_id=470650 H3L98_RS05220 WP_027021067.1 940675..942354(-) (pilF) [Conchiformibius steedae strain DSM 2580]
MSIGLFRVLLQQKLIPQEKVSKYQQAAENNSFLQTIFNDKIITPQDLSSLLSRMFNYPQFNLNAFSRRGLMTDLLDENQM
AEHRCVPLFKRGKKLFMGVSDPTNIQALQKLVFSSGLSVDLVLVRDDHLSNFLEWFGQSSTSLLREMGADSALQEAQAQT
MIVDGEEEEDGPIARFIQKVLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPLNLRSQLASRIKVMAKLDISER
RVPQDGRIQLQFGKGTKAIDFRVNTMPCLFGEKVVMRILNSDAATLNIDQLGFEDFQKELIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLARRLCSSCKEPMERPPEPALKRAGFTDEDLAGDWVM
YRPVGCEVCKGKGYKGRAGLYEVMPMTEKMKAVIMNNGTEVDIANIAYEEGLVDLRRSGLLKVMQGITSLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=470650 H3L98_RS05220 WP_027021067.1 940675..942354(-) (pilF) [Conchiformibius steedae strain DSM 2580]
ATGAGCATCGGTTTATTTCGTGTTCTGCTGCAACAGAAGCTGATACCGCAAGAAAAAGTCAGCAAATACCAACAAGCAGC
CGAAAACAACAGCTTTCTGCAAACCATTTTTAACGACAAAATCATTACCCCCCAAGATTTGTCGTCTTTGCTGTCGCGCA
TGTTCAACTACCCGCAGTTTAACCTTAATGCGTTTTCGCGGCGCGGGCTGATGACCGATTTGCTGGACGAAAACCAAATG
GCGGAACACCGCTGCGTACCTTTGTTCAAACGCGGCAAAAAACTGTTTATGGGCGTATCCGACCCCACCAATATCCAAGC
ACTGCAAAAACTGGTGTTTTCCAGCGGTTTGTCTGTTGATTTGGTGCTGGTGCGCGATGACCACTTAAGCAATTTTCTGG
AATGGTTCGGACAAAGCAGCACCAGCCTGTTGCGCGAAATGGGCGCAGACAGCGCCTTGCAGGAAGCCCAGGCGCAAACC
ATGATTGTGGACGGCGAGGAAGAAGAAGACGGTCCCATCGCCCGCTTTATCCAAAAAGTCTTGTCAGACGCGCTCAATGC
AGGCGCATCGGATATCCATTTTGAATTTTACGAACAAATGGCACGGGTGCGTTTCCGTGTGGACGGTCAGTTGCGCGAAG
TGGTGCAACCGCCGCTCAACCTGCGTAGCCAGTTGGCATCGCGTATTAAAGTAATGGCAAAACTGGATATTTCCGAACGA
CGCGTACCGCAGGACGGACGTATCCAGCTGCAATTTGGTAAAGGCACGAAAGCCATTGACTTCCGTGTGAACACCATGCC
CTGCCTGTTTGGCGAAAAAGTGGTGATGCGTATTTTGAACTCCGATGCCGCCACGCTGAACATCGACCAACTGGGTTTTG
AAGACTTCCAAAAAGAATTGATTATGGAAGCCATTCACCGTCCTTACGGCATGGTGTTGGTAACAGGTCCTACGGGTTCG
GGTAAAACCGTATCACTTTATACCTGCCTGAACATTCTGAATACCGACGATGTGAACATTTCCACCGCCGAAGACCCCGC
CGAGATTAACTTACCTGGTATCAATCAGGTAAACGTCAACGACAAACAGGGCTTAACCTTTGAAGCGGCACTCAAAGCCT
TCCTGCGTCAAGACCCTGACATCATCATGATTGGTGAGATTCGTGACTTGGGTACTGCCGACATCGCCATTAAAGCCGCC
CAAACGGGTCACATGGTATTTTCCACCCTGCACACCAACAACGCCCCCGCCACCCTGTCGCGTATGCTGAACATGGGTGT
TGCCCCGTTTAACATCGCCAGTTCCGTCAATCTAATTATGGCGCAGCGTTTGGCACGTCGTCTCTGTTCTTCCTGCAAAG
AACCCATGGAACGTCCGCCCGAACCCGCCCTCAAACGCGCAGGCTTTACCGATGAAGATTTGGCAGGCGATTGGGTGATG
TATCGTCCTGTAGGCTGCGAAGTGTGCAAAGGCAAAGGCTATAAAGGACGGGCGGGTTTGTATGAAGTGATGCCGATGAC
CGAAAAAATGAAAGCCGTGATTATGAACAACGGTACCGAAGTCGATATTGCCAATATTGCCTACGAAGAAGGCTTGGTGG
ATTTGCGCCGTTCCGGTTTGCTGAAAGTGATGCAAGGCATTACCTCGCTTGAAGAAGTGATTGCCACCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.036

100

0.682
  pilB Acinetobacter baumannii D1279779

52.135

100

0.524
  pilB Acinetobacter baylyi ADP1

51.805

99.106

0.513
  pilB Legionella pneumophila strain ERS1305867

47.965

100

0.485
  pilB Vibrio cholerae strain A1552

45.745

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.458
  pilB Vibrio campbellii strain DS40M4

45.273

98.39

0.445