Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SA190iCDA_RS05880 Genome accession   NZ_CP056087
Coordinates   1298725..1300428 (-) Length   567 a.a.
NCBI ID   WP_070884461.1    Uniprot ID   -
Organism   Pseudomonas argentinensis strain SA190     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1293725..1305428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA190iCDA_RS05855 - 1295142..1295669 (-) 528 WP_070884464.1 MOSC domain-containing protein -
  SA190iCDA_RS05860 yacG 1295709..1295909 (-) 201 WP_070884463.1 DNA gyrase inhibitor YacG -
  SA190iCDA_RS05865 coaE 1295906..1296514 (-) 609 WP_070884681.1 dephospho-CoA kinase -
  SA190iCDA_RS05870 pilD 1296623..1297495 (-) 873 WP_139159445.1 prepilin peptidase Machinery gene
  SA190iCDA_RS05875 pilC 1297499..1298722 (-) 1224 WP_070884462.1 type II secretion system F family protein Machinery gene
  SA190iCDA_RS05880 pilB 1298725..1300428 (-) 1704 WP_070884461.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SA190iCDA_RS05885 pilA2 1300672..1301097 (+) 426 WP_083329721.1 pilin Machinery gene
  SA190iCDA_RS05890 - 1301159..1303099 (+) 1941 WP_205881589.1 tetratricopeptide repeat protein -
  SA190iCDA_RS05895 - 1303144..1303995 (+) 852 WP_070884460.1 glycosyltransferase family 2 protein -
  SA190iCDA_RS05900 - 1304003..1304236 (+) 234 WP_070884459.1 helix-turn-helix domain-containing protein -
  SA190iCDA_RS05905 - 1304239..1305222 (+) 984 WP_236101044.1 lipopolysaccharide biosynthesis protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62409.26 Da        Isoelectric Point: 5.6080

>NTDB_id=458122 SA190iCDA_RS05880 WP_070884461.1 1298725..1300428(-) (pilB) [Pseudomonas argentinensis strain SA190]
MSESIPLSGLAKQVVLAELLDEKAAQQAQVQAKRNKLSLVHYLAQNKLVKSRALAELTADQFGIAYFDLSALDKESQPKD
LVSEKLVRQHRALPLWRRGNKLFVGISDPSNHQVVTDIQFSTGLSTEALLVEDDKLGDAIDKFFDTGHTGLEDMADVDLD
GLETESVDDDKKSDGGGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERAYRVRFRTDGILHETARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKISKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEEDQKELYLNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSSCKKEVDIPREALLEEGFP
EDKIGSFKIYGPVGCENCKGGYKGRVGIYEVVKNTPSLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=458122 SA190iCDA_RS05880 WP_070884461.1 1298725..1300428(-) (pilB) [Pseudomonas argentinensis strain SA190]
ATGAGCGAGAGCATCCCCCTTTCCGGCCTGGCCAAGCAGGTAGTGCTAGCTGAGCTGCTGGACGAGAAGGCCGCTCAACA
GGCCCAAGTACAAGCTAAGCGAAACAAACTGTCTCTGGTTCATTACCTGGCTCAGAACAAGCTGGTGAAAAGTCGCGCAC
TGGCAGAGCTGACTGCGGATCAATTTGGCATCGCTTATTTCGACCTCAGCGCGCTAGACAAGGAAAGTCAGCCCAAGGAT
CTGGTCAGTGAAAAGCTGGTGCGCCAGCATCGCGCGCTGCCCTTATGGCGCCGTGGCAACAAGCTGTTCGTAGGCATCTC
GGATCCCTCCAATCACCAAGTCGTTACCGATATCCAGTTCAGCACAGGACTCTCTACAGAAGCGTTGCTGGTTGAGGACG
ACAAGCTGGGCGACGCCATCGATAAGTTTTTCGATACCGGCCATACGGGGCTGGAAGACATGGCCGACGTTGATCTCGAT
GGCCTGGAAACCGAGTCGGTCGATGACGACAAAAAGAGCGACGGTGGTGGCCAGGACGCCGATGATGCCCCGGTGGTTCG
TTTCGTCAACAAGATGCTGCTCGATGCCATCAAAGGTGGCTCCTCGGATCTGCACTTCGAGCCCTATGAGCGCGCTTACC
GCGTGCGTTTTCGTACTGACGGCATCCTGCACGAGACGGCGCGCCCGCCTATCCAGCTGGCACCGCGCATTTCCGCGCGC
CTGAAGGTCATGGCCGGCCTTGATATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGTATCAAGATGAAGATTTCCAAGAG
CAAATCCATCGACTTCCGCGTCAACACCTTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATTCTGCCA
GCGCACAGATGGGTATCGACGCCCTGGGTTACGAGGAAGACCAGAAGGAACTGTACCTCAACGCCCTCAAGCAGCCGCAA
GGCATGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTATCTCTGTATACCGGCCTCAATATTCTCAATACCCC
AGATGTGAATATTTCCACGGCTGAAGACCCTGTGGAAATCAACCTGGAAGGCATCAACCAAGTCAACGTCAACCCCAAAC
AGGGGATGGACTTTACCCAGGCACTGCGCGCCTTCCTGCGTCAGGATCCGGATATCATCATGGTCGGTGAGATTCGTGAC
CTGGACACCGCCTCCATCGCCATCAAGGCCGCGCAGACCGGCCATATGGTGATGTCGACCCTGCACACCAATAGCGCTGC
GGAGACGCTAACTCGCCTACGTAATATGGGTGTGCCCTCGTTCAACATTGCCACCTCGGTCAACCTGATCATTGCCCAGC
GCCTGGCGCGCAAGCTGTGTTCGTCCTGCAAAAAGGAAGTCGATATCCCGCGCGAAGCATTGCTGGAGGAAGGCTTCCCG
GAAGACAAGATAGGTAGCTTCAAGATCTACGGCCCGGTTGGCTGCGAAAACTGCAAGGGCGGCTATAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGAGCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCGCGC
AAATGCGCAAAGACGGCTTTAACGACCTGCGCACCTCGGCCCTTGTAAAGGCCATGCAAGGCGTGACCAGCCTTGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.536

98.765

0.598
  pilB Acinetobacter baylyi ADP1

58.156

99.471

0.578
  pilB Legionella pneumophila strain ERS1305867

55.088

100

0.554
  pilF Neisseria gonorrhoeae MS11

48.49

99.295

0.481
  pilB Vibrio cholerae strain A1552

49.455

97.002

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

50.464

95.062

0.48
  pilB Vibrio campbellii strain DS40M4

49.084

96.296

0.473
  pilB/pilB1 Synechocystis sp. PCC 6803

36.187

100

0.395
  pilF Thermus thermophilus HB27

37.39

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.015

93.122

0.363