Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HUW35_RS00830 Genome accession   NZ_CP055157
Coordinates   177240..178454 (+) Length   404 a.a.
NCBI ID   WP_181253845.1    Uniprot ID   A0A7H8PUF5
Organism   Microbulbifer sp. YPW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 172240..183454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW35_RS00815 (HUW35_00815) - 172581..174347 (-) 1767 WP_181253842.1 hypothetical protein -
  HUW35_RS00820 (HUW35_00820) - 174420..174857 (-) 438 WP_181253843.1 pilin -
  HUW35_RS00825 (HUW35_00825) pilB 175372..177084 (+) 1713 WP_181253844.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUW35_RS00830 (HUW35_00830) pilC 177240..178454 (+) 1215 WP_181253845.1 type II secretion system F family protein Machinery gene
  HUW35_RS00835 (HUW35_00835) - 178473..179357 (+) 885 WP_181253846.1 A24 family peptidase -
  HUW35_RS00840 (HUW35_00840) coaE 179362..179973 (+) 612 WP_181253847.1 dephospho-CoA kinase -
  HUW35_RS00845 (HUW35_00845) yacG 179988..180194 (+) 207 WP_181253848.1 DNA gyrase inhibitor YacG -
  HUW35_RS00850 (HUW35_00850) - 180213..180746 (-) 534 WP_181253849.1 protein disulfide oxidoreductase -
  HUW35_RS00855 (HUW35_00855) tsaA 180743..181486 (-) 744 WP_255463402.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  HUW35_RS00860 (HUW35_00860) - 181483..182940 (-) 1458 WP_181253850.1 NAD-dependent succinate-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43551.39 Da        Isoelectric Point: 9.6228

>NTDB_id=456432 HUW35_RS00830 WP_181253845.1 177240..178454(+) (pilC) [Microbulbifer sp. YPW1]
MAKAATAVAYIYKGVDAKGNKVEGEINSTNPALIKAQLRRQGIIANRVQKKPKPLFGGKKKVKPGDIAIFTRQMATMMRA
GVPLVQSFEIVADGLDNEGLKELIFKIRDDVASGTAFADALRRHPLYFDDLFCNLVASGEQSGALETMLDRIATYKEKTE
SLKAKIKKAMTYPIAVVVVAIVVTAILLVKVVPQFAETFSSFGADLPAFTLFVLGLSEWMQANWLIAFVALVVGVGGTLE
AKKRNKKVANFFDKLMLRLPIIGQITYNSIAARFARTLSTTFAAGVPLIDALKSVAGATGNVVYEVDTLKIRDSVATGIP
LNGAMRASGLFPTMLVQMAAIGEESGALDEMLAKAADFYEEAVDNMVDNLTTLMEPMIMAVLGILVGGLMIAMYLPIFQL
GQVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=456432 HUW35_RS00830 WP_181253845.1 177240..178454(+) (pilC) [Microbulbifer sp. YPW1]
ATGGCCAAAGCCGCCACCGCAGTCGCTTATATTTATAAAGGCGTCGACGCCAAAGGCAATAAAGTTGAGGGTGAGATCAA
TAGTACCAACCCTGCGTTGATCAAGGCCCAGCTGCGCCGCCAGGGGATTATTGCCAACAGGGTACAAAAGAAGCCGAAGC
CTTTGTTTGGTGGCAAGAAGAAAGTGAAGCCCGGCGATATTGCTATCTTCACCCGCCAAATGGCGACAATGATGCGGGCC
GGTGTGCCGCTGGTGCAGAGCTTTGAAATTGTAGCCGATGGCCTGGACAACGAGGGGCTGAAAGAGCTCATTTTCAAAAT
CCGTGATGATGTCGCTTCCGGGACCGCCTTCGCCGACGCCCTGCGCAGGCACCCGCTCTACTTTGATGACTTGTTCTGTA
ACCTTGTCGCCTCGGGCGAGCAATCCGGGGCGCTGGAAACAATGCTCGATCGGATCGCAACCTACAAAGAAAAGACAGAG
TCACTGAAGGCAAAAATCAAAAAGGCCATGACTTACCCTATTGCGGTGGTTGTGGTGGCGATTGTGGTAACGGCTATTCT
ACTCGTAAAAGTTGTGCCGCAATTCGCTGAGACTTTTTCCAGCTTTGGCGCTGATCTGCCAGCCTTCACGCTTTTCGTCC
TGGGCCTTTCCGAGTGGATGCAGGCAAACTGGCTTATCGCTTTTGTAGCACTGGTAGTCGGAGTGGGTGGAACTCTCGAA
GCGAAAAAGCGTAACAAGAAAGTCGCCAACTTCTTTGACAAGCTGATGCTGCGCCTGCCGATTATTGGGCAGATTACCTA
CAATTCGATTGCCGCTCGCTTCGCTCGAACGCTGTCAACAACCTTCGCCGCGGGCGTTCCTCTTATTGACGCACTGAAGT
CCGTAGCCGGTGCAACCGGGAATGTAGTTTACGAAGTAGATACCCTCAAAATTCGTGATTCTGTTGCAACAGGTATCCCA
CTTAATGGCGCAATGCGAGCATCGGGTCTCTTCCCTACCATGCTGGTACAAATGGCCGCGATTGGTGAAGAGTCTGGCGC
GCTGGACGAAATGCTGGCCAAGGCAGCGGACTTTTATGAAGAGGCTGTTGACAATATGGTCGACAACCTTACAACGCTGA
TGGAGCCCATGATCATGGCGGTCCTGGGTATTCTGGTTGGCGGCCTGATGATTGCCATGTACCTCCCCATCTTCCAACTC
GGTCAGGTTGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8PUF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.687

99.505

0.624
  pilC Acinetobacter baylyi ADP1

60.784

100

0.614
  pilC Acinetobacter baumannii D1279779

60.396

100

0.604
  pilC Legionella pneumophila strain ERS1305867

56.784

98.515

0.559
  pilG Neisseria gonorrhoeae MS11

42.929

98.02

0.421
  pilG Neisseria meningitidis 44/76-A

42.929

98.02

0.421
  pilC Vibrio cholerae strain A1552

41.667

98.02

0.408
  pilC Vibrio campbellii strain DS40M4

40.25

99.01

0.399
  pilC Thermus thermophilus HB27

35.561

100

0.369