Detailed information NTG0456431

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HUW35_RS00825
• Genome accession: NZ_CP055157
• Coordinates: 175372..177084 (+)
• Length: 570 a.a.
• NCBI ID: WP_181253844.1
• Uniprot ID: A0A7H8PTW6
• Organism: Microbulbifer sp. YPW1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	162291..174857	175372..177084	flank	515

Gene organization within MGE regions

Location: 162291..177084

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUW35_RS00760 (HUW35_00760)	-	162465..163235 (+)	771	WP_255463399.1	glycerophosphodiester phosphodiesterase	-
HUW35_RS00765 (HUW35_00765)	-	163264..163923 (-)	660	WP_181253834.1	peroxiredoxin-like family protein	-
HUW35_RS00770 (HUW35_00770)	-	164143..164718 (-)	576	WP_181253835.1	hypothetical protein	-
HUW35_RS00775 (HUW35_00775)	-	165106..165867 (-)	762	WP_181253836.1	hypothetical protein	-
HUW35_RS18810	-	165979..166113 (-)	135	WP_255463400.1	hypothetical protein	-
HUW35_RS18955 (HUW35_00780)	-	166321..166446 (-)	126	Protein_151	DUF465 domain-containing protein	-
HUW35_RS00785 (HUW35_00785)	-	166701..167438 (-)	738	WP_181253837.1	SDR family oxidoreductase	-
HUW35_RS00790 (HUW35_00790)	-	167441..168742 (-)	1302	WP_181253838.1	FAD-binding oxidoreductase	-
HUW35_RS00795 (HUW35_00795)	-	168739..169143 (-)	405	WP_181253839.1	GtrA family protein	-
HUW35_RS00800 (HUW35_00800)	-	169179..170066 (+)	888	WP_255463401.1	decaprenyl-phosphate phosphoribosyltransferase	-
HUW35_RS00805 (HUW35_00805)	-	170075..170506 (+)	432	WP_181253840.1	divergent PAP2 family protein	-
HUW35_RS00810 (HUW35_00810)	-	170726..172387 (-)	1662	WP_181253841.1	hypothetical protein	-
HUW35_RS00815 (HUW35_00815)	-	172581..174347 (-)	1767	WP_181253842.1	hypothetical protein	-
HUW35_RS00820 (HUW35_00820)	-	174420..174857 (-)	438	WP_181253843.1	pilin	-
HUW35_RS00825 (HUW35_00825)	pilB	175372..177084 (+)	1713	WP_181253844.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 570 a.a.
• Molecular weight: 62364.09 Da
• Isoelectric Point: 5.1718

>NTDB_id=456431 HUW35_RS00825 WP_181253844.1 175372..177084(+) (pilB) [Microbulbifer sp. YPW1]
MSSTPLSGLAKRLVSDNAIDEATAQSAIKAAKREGQTFAQHAVEAKLVKSRDLANIASTAFGSPLFDLSSYNFDLLPKDV
VDEKLISKHFALPLYKRGNRLFVAVADPTNLAGLDEINFNTGLNTDAILVETDKLAKAIESYLANSGDIGGGLEGLDDED
LDSLDVESGENRQDDDKDPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKLYRVRLRTDGVLHEVARPPIQLATRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEESQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCGECKKPVELPEEVLKEEGF
DTVTIPESEWHIFQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIEIADQARKEGFNNLRTSALRKVVMGVTS
LEEANRVTKD

Nucleotide

• Length: 1713 bp

>NTDB_id=456431 HUW35_RS00825 WP_181253844.1 175372..177084(+) (pilB) [Microbulbifer sp. YPW1]
ATGAGCAGCACTCCACTGAGCGGCCTGGCCAAGCGGCTGGTTTCTGACAATGCTATCGATGAGGCTACTGCCCAGTCGGC
GATCAAGGCGGCCAAACGTGAGGGGCAGACCTTTGCCCAGCACGCGGTGGAGGCCAAACTGGTCAAGAGCCGAGATCTGG
CCAATATCGCATCAACGGCATTTGGTAGCCCCCTGTTTGACCTTTCGAGCTACAACTTTGACCTCCTTCCCAAGGATGTT
GTTGACGAAAAGTTGATCAGCAAGCACTTTGCGCTCCCTCTTTATAAGCGCGGCAACCGGCTTTTTGTCGCAGTAGCAGA
CCCCACCAATCTCGCAGGCCTGGACGAAATCAACTTCAACACCGGCCTGAACACCGATGCCATCCTGGTAGAAACGGACA
AGCTGGCCAAAGCCATCGAAAGTTATCTTGCTAATAGTGGCGACATAGGCGGTGGCCTGGAAGGGTTGGACGATGAGGAC
CTGGACAGTCTGGATGTTGAGAGCGGGGAAAACCGGCAGGATGACGATAAGGACCCCGGAGGGGATGAGGCCCCAGTTGT
ACGTTTTGTAAACAAGGTGCTATTGGATGCGATCCGTACCGGGGCGTCCGACATACACTTTGAGCCCTATGAAAAGCTTT
ATCGGGTACGCCTGCGCACCGACGGCGTACTTCATGAAGTCGCCCGTCCACCCATCCAGTTGGCGACCCGGATCTCTGCC
CGCTTGAAAGTTATGTCCAAAATGGACATTTCTGAGCGCCGGGTACCCCAGGACGGCCGTATCAAGATGAAGCTGTCCAA
GACCAAGGCCATCGACTTCCGGGTAAACAGCCTCCCGACTCTTTGGGGAGAAAAGATCGTTCTGCGGATACTCGACCCCT
CTTCTGCCAAGCTGGGCATCGATGCTCTCGGCTACGAAGAGAGCCAGAAAAAGATCTATATGGATGCTCTTGCGCAGCCG
CAGGGCATGATCCTGGTGACCGGCCCTACCGGCTCCGGAAAAACCGTATCGCTATACACAGGCCTGAATATTCTGAACAC
CCCGGAGCGCAATATCTCCACGGCGGAAGACCCGGTAGAAATCAACCTGGAGGGTATTAATCAGGTAAACGTCTCCAACA
AGGTGGGACTTAACTTCGCTGAGGCGCTGCGCTCCTTTCTGCGTCAGGACCCGGATATTGTGATGGTGGGGGAGATTCGG
GACTTGGAAACGGCAGAAATCGCCATCAAGGCGGCACAGACCGGGCACCTTGTACTCTCTACCCTTCACACTAACTCGGC
CCCGGAAACCCTGACGCGACTGATGAATATGGGGGTTCCGACTTTCAATATCGCGACGTCTGTCAGTGTCATTATCGCCC
AGCGTCTTGCACGACGGCTCTGCGGCGAGTGCAAGAAGCCGGTAGAGTTACCCGAGGAGGTATTGAAGGAGGAGGGTTTC
GATACGGTCACCATCCCCGAAAGCGAGTGGCATATATTCCAGCCCGTCGGTTGCGAGCACTGTTCGAAAGGCTACAAGGG
GCGCGTCGGCGTGTATGAAGTGGTTCGCATCACCGACGGAATCTCGCGCATTATAATGGAGGGTGGCAACTCCATTGAGA
TTGCCGACCAGGCCAGGAAAGAAGGCTTCAACAACCTCCGTACTTCTGCGCTGCGGAAGGTAGTAATGGGGGTTACAAGC
CTTGAGGAAGCTAACCGGGTAACGAAAGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (192-459)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

3D structure

• AlphaFold DB: A0A7H8PTW6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	61.646	100	0.618
pilB	Acinetobacter baylyi ADP1	60.07	100	0.602
pilB	Legionella pneumophila strain ERS1305867	54.497	99.474	0.542
pilB	Vibrio parahaemolyticus RIMD 2210633	53.663	95.789	0.514
pilB	Vibrio campbellii strain DS40M4	51.504	99.123	0.511
pilB	Vibrio cholerae strain A1552	50.973	99.123	0.505
pilF	Neisseria gonorrhoeae MS11	49.336	92.456	0.456
pilB/pilB1	Synechocystis sp. PCC 6803	37.886	100	0.409
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.036	98.246	0.374