Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HUK75_RS24810 Genome accession   NZ_CP054792
Coordinates   5276737..5277954 (+) Length   405 a.a.
NCBI ID   WP_016254080.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5431     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5262500..5277954 5276737..5277954 within 0


Gene organization within MGE regions


Location: 5262500..5277954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK75_RS24725 (HUK75_24670) - 5262500..5263720 (+) 1221 WP_062832571.1 tyrosine-type recombinase/integrase -
  HUK75_RS24730 (HUK75_24675) - 5263792..5264613 (-) 822 WP_062832572.1 hypothetical protein -
  HUK75_RS31270 - 5264928..5265053 (+) 126 WP_265324936.1 hypothetical protein -
  HUK75_RS24735 (HUK75_24680) - 5265182..5265343 (+) 162 WP_162998314.1 hypothetical protein -
  HUK75_RS24740 (HUK75_24685) - 5265755..5266069 (+) 315 WP_062832573.1 helix-turn-helix transcriptional regulator -
  HUK75_RS24745 (HUK75_24690) - 5266073..5268472 (+) 2400 WP_079767670.1 bifunctional DNA primase/polymerase -
  HUK75_RS24750 (HUK75_24695) - 5269063..5269578 (+) 516 WP_062832575.1 hypothetical protein -
  HUK75_RS24755 (HUK75_24700) - 5269568..5269816 (+) 249 WP_062832576.1 hypothetical protein -
  HUK75_RS24760 (HUK75_24705) - 5269791..5270237 (+) 447 WP_062832577.1 hypothetical protein -
  HUK75_RS24765 (HUK75_24710) - 5270463..5271179 (+) 717 WP_062832578.1 hypothetical protein -
  HUK75_RS24770 (HUK75_24720) - 5271172..5271447 (+) 276 WP_050714322.1 hypothetical protein -
  HUK75_RS24775 (HUK75_24725) - 5271665..5272132 (+) 468 WP_062832579.1 hypothetical protein -
  HUK75_RS24780 (HUK75_24730) - 5272126..5272506 (+) 381 WP_062832580.1 hypothetical protein -
  HUK75_RS24785 (HUK75_24735) - 5272597..5273064 (-) 468 WP_062832581.1 hypothetical protein -
  HUK75_RS24795 (HUK75_24745) - 5273498..5274196 (-) 699 WP_016254081.1 hypothetical protein -
  HUK75_RS24800 (HUK75_24750) - 5274259..5274801 (-) 543 WP_235179930.1 pilin -
  HUK75_RS24805 (HUK75_24755) pilB 5275030..5276733 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK75_RS24810 (HUK75_24760) pilC 5276737..5277954 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44041.92 Da        Isoelectric Point: 9.8417

>NTDB_id=454671 HUK75_RS24810 WP_016254080.1 5276737..5277954(+) (pilC) [Pseudomonas aeruginosa strain SE5431]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEMLQEWWLIVLIGLFAAAFAFR
EAHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=454671 HUK75_RS24810 WP_016254080.1 5276737..5277954(+) (pilC) [Pseudomonas aeruginosa strain SE5431]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAAGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTATCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGTGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATGCTTCAAGAGTGGTGGCTCATAGTGCTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGCTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.753

100

0.798
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Legionella pneumophila strain ERS1305867

55.303

97.778

0.541
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38