Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUK75_RS24805 Genome accession   NZ_CP054792
Coordinates   5275030..5276733 (+) Length   567 a.a.
NCBI ID   WP_003141354.1    Uniprot ID   A0A0H2ZH73
Organism   Pseudomonas aeruginosa strain SE5431     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5262500..5277954 5275030..5276733 within 0


Gene organization within MGE regions


Location: 5262500..5277954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK75_RS24725 (HUK75_24670) - 5262500..5263720 (+) 1221 WP_062832571.1 tyrosine-type recombinase/integrase -
  HUK75_RS24730 (HUK75_24675) - 5263792..5264613 (-) 822 WP_062832572.1 hypothetical protein -
  HUK75_RS31270 - 5264928..5265053 (+) 126 WP_265324936.1 hypothetical protein -
  HUK75_RS24735 (HUK75_24680) - 5265182..5265343 (+) 162 WP_162998314.1 hypothetical protein -
  HUK75_RS24740 (HUK75_24685) - 5265755..5266069 (+) 315 WP_062832573.1 helix-turn-helix transcriptional regulator -
  HUK75_RS24745 (HUK75_24690) - 5266073..5268472 (+) 2400 WP_079767670.1 bifunctional DNA primase/polymerase -
  HUK75_RS24750 (HUK75_24695) - 5269063..5269578 (+) 516 WP_062832575.1 hypothetical protein -
  HUK75_RS24755 (HUK75_24700) - 5269568..5269816 (+) 249 WP_062832576.1 hypothetical protein -
  HUK75_RS24760 (HUK75_24705) - 5269791..5270237 (+) 447 WP_062832577.1 hypothetical protein -
  HUK75_RS24765 (HUK75_24710) - 5270463..5271179 (+) 717 WP_062832578.1 hypothetical protein -
  HUK75_RS24770 (HUK75_24720) - 5271172..5271447 (+) 276 WP_050714322.1 hypothetical protein -
  HUK75_RS24775 (HUK75_24725) - 5271665..5272132 (+) 468 WP_062832579.1 hypothetical protein -
  HUK75_RS24780 (HUK75_24730) - 5272126..5272506 (+) 381 WP_062832580.1 hypothetical protein -
  HUK75_RS24785 (HUK75_24735) - 5272597..5273064 (-) 468 WP_062832581.1 hypothetical protein -
  HUK75_RS24795 (HUK75_24745) - 5273498..5274196 (-) 699 WP_016254081.1 hypothetical protein -
  HUK75_RS24800 (HUK75_24750) - 5274259..5274801 (-) 543 WP_235179930.1 pilin -
  HUK75_RS24805 (HUK75_24755) pilB 5275030..5276733 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK75_RS24810 (HUK75_24760) pilC 5276737..5277954 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=454670 HUK75_RS24805 WP_003141354.1 5275030..5276733(+) (pilB) [Pseudomonas aeruginosa strain SE5431]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFP
EDKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=454670 HUK75_RS24805 WP_003141354.1 5275030..5276733(+) (pilB) [Pseudomonas aeruginosa strain SE5431]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTAATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCAGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCAGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCTA
GCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAATCTGATCATCGCCCAGC
GCCTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCG
GAAGACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZH73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.58
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376