Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HUE57_RS10295 Genome accession   NZ_CP054491
Coordinates   1844928..1845725 (+) Length   265 a.a.
NCBI ID   WP_174673127.1    Uniprot ID   A0A6N0HW74
Organism   Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1839928..1850725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUE57_RS10270 (HUE57_10270) - 1841629..1842300 (+) 672 WP_236860572.1 endonuclease III domain-containing protein -
  HUE57_RS10275 (HUE57_10275) - 1842319..1842492 (-) 174 WP_174673124.1 hypothetical protein -
  HUE57_RS10280 (HUE57_10280) - 1842494..1843432 (-) 939 WP_078483379.1 GGDEF domain-containing protein -
  HUE57_RS10285 (HUE57_10285) - 1843694..1844647 (+) 954 WP_174673125.1 GspE/PulE family protein -
  HUE57_RS10290 (HUE57_10290) - 1844703..1844897 (-) 195 WP_174673126.1 hypothetical protein -
  HUE57_RS10295 (HUE57_10295) pilF 1844928..1845725 (+) 798 WP_174673127.1 GspE/PulE family protein Machinery gene
  HUE57_RS10300 (HUE57_10300) - 1846973..1847296 (+) 324 WP_078483381.1 low molecular weight protein tyrosine phosphatase family protein -
  HUE57_RS10305 (HUE57_10305) - 1847421..1847666 (+) 246 WP_174673128.1 hypothetical protein -
  HUE57_RS10310 (HUE57_10310) - 1847644..1847928 (-) 285 WP_174673129.1 hypothetical protein -
  HUE57_RS10315 (HUE57_10315) - 1848142..1848879 (-) 738 WP_078483383.1 YciK family oxidoreductase -
  HUE57_RS10320 (HUE57_10320) hpnE 1848900..1850237 (-) 1338 WP_078483384.1 hydroxysqualene dehydroxylase HpnE -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29277.52 Da        Isoelectric Point: 5.3730

>NTDB_id=452876 HUE57_RS10295 WP_174673127.1 1844928..1845725(+) (pilF) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
MLGFGEHEVDTLRDLITKPHGMVLVTGPTGSGKSTTLYSLLNEVKRQNPHIITVEDPVEYAMDGVEQIQISNVKGYTFAE
ALRHILRHDPDVIMVGEIRDLETAQIANKAALTGHLMLSTLHTNDAISSVTRLIDMGVEPFLLSSTLLGAMAQRLVRVIC
EECKEVDTIDTKLRQTLGVSDDEIFYRGRGCPTCSQSGYRGRVAVCEIFTVTPEIQQLIHANKGEQAIREALHKAGMTTL
FEDALELAREGRTTLEEAYKVRTVG

Nucleotide


Download         Length: 798 bp        

>NTDB_id=452876 HUE57_RS10295 WP_174673127.1 1844928..1845725(+) (pilF) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
GTGCTCGGATTTGGCGAACATGAGGTCGATACCCTGCGTGATCTGATCACCAAACCGCACGGCATGGTGCTGGTCACCGG
GCCGACCGGCTCTGGTAAGTCGACCACCCTCTACTCACTGCTCAATGAGGTGAAACGGCAAAATCCGCACATCATCACCG
TCGAAGATCCGGTTGAGTATGCGATGGACGGCGTCGAGCAGATTCAAATCTCAAACGTGAAGGGTTACACCTTCGCCGAG
GCACTGCGCCACATCCTGCGCCACGACCCCGATGTGATTATGGTCGGCGAGATTCGTGACCTGGAAACGGCACAGATCGC
CAACAAGGCTGCACTCACCGGACACCTGATGCTCTCCACTCTTCACACCAATGATGCGATCAGCTCCGTAACACGATTGA
TCGACATGGGTGTCGAACCGTTCCTACTGAGCTCCACACTGCTAGGCGCAATGGCGCAACGACTGGTGCGCGTTATCTGC
GAAGAGTGCAAAGAGGTTGATACCATCGATACGAAACTGCGTCAGACACTCGGCGTTTCCGATGATGAGATCTTCTATCG
TGGAAGAGGATGCCCCACATGTAGCCAGAGCGGCTACCGTGGCCGAGTCGCCGTCTGTGAAATCTTCACCGTAACGCCAG
AGATCCAACAGCTGATCCACGCCAATAAAGGTGAGCAGGCAATCAGAGAGGCGCTTCACAAGGCGGGAATGACAACACTC
TTTGAAGATGCGCTTGAGCTAGCCAGAGAGGGTCGTACCACCCTGGAAGAGGCCTACAAAGTCCGAACCGTCGGTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(2-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

49.615

98.113

0.487
  pilB/pilB1 Synechocystis sp. PCC 6803

46.324

100

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.415

100

0.464
  pilB Legionella pneumophila strain ERS1305867

45.283

100

0.453
  pilB Vibrio cholerae strain A1552

44.656

98.868

0.442
  pilF Neisseria gonorrhoeae MS11

43.893

98.868

0.434
  ctsE Campylobacter jejuni subsp. jejuni 81-176

43.462

98.113

0.426
  pilB Acinetobacter baumannii D1279779

41.353

100

0.415
  pilB Acinetobacter baylyi ADP1

40.602

100

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

41.221

98.868

0.408
  pilB Vibrio campbellii strain DS40M4

40.458

98.868

0.4
  pilB Haemophilus influenzae 86-028NP

41.004

90.189

0.37
  pilB Glaesserella parasuis strain SC1401

45.498

79.623

0.362
  pilT Acinetobacter baylyi ADP1

35.955

100

0.362