Detailed information NTG0452876

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: HUE57_RS10295
• Genome accession: NZ_CP054491
• Coordinates: 1844928..1845725 (+)
• Length: 265 a.a.
• NCBI ID: WP_174673127.1
• Uniprot ID: A0A6N0HW74
• Organism: Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Integron	1846086..1847406	1844928..1845725	flank	361

Gene organization within MGE regions

Location: 1844928..1847406

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUE57_RS10295 (HUE57_10295)	pilF	1844928..1845725 (+)	798	WP_174673127.1	GspE/PulE family protein	Machinery gene
HUE57_RS10300 (HUE57_10300)	-	1846973..1847296 (+)	324	WP_078483381.1	low molecular weight protein tyrosine phosphatase family protein	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29277.52 Da
• Isoelectric Point: 5.3730

>NTDB_id=452876 HUE57_RS10295 WP_174673127.1 1844928..1845725(+) (pilF) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
MLGFGEHEVDTLRDLITKPHGMVLVTGPTGSGKSTTLYSLLNEVKRQNPHIITVEDPVEYAMDGVEQIQISNVKGYTFAE
ALRHILRHDPDVIMVGEIRDLETAQIANKAALTGHLMLSTLHTNDAISSVTRLIDMGVEPFLLSSTLLGAMAQRLVRVIC
EECKEVDTIDTKLRQTLGVSDDEIFYRGRGCPTCSQSGYRGRVAVCEIFTVTPEIQQLIHANKGEQAIREALHKAGMTTL
FEDALELAREGRTTLEEAYKVRTVG

Nucleotide

• Length: 798 bp

>NTDB_id=452876 HUE57_RS10295 WP_174673127.1 1844928..1845725(+) (pilF) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
GTGCTCGGATTTGGCGAACATGAGGTCGATACCCTGCGTGATCTGATCACCAAACCGCACGGCATGGTGCTGGTCACCGG
GCCGACCGGCTCTGGTAAGTCGACCACCCTCTACTCACTGCTCAATGAGGTGAAACGGCAAAATCCGCACATCATCACCG
TCGAAGATCCGGTTGAGTATGCGATGGACGGCGTCGAGCAGATTCAAATCTCAAACGTGAAGGGTTACACCTTCGCCGAG
GCACTGCGCCACATCCTGCGCCACGACCCCGATGTGATTATGGTCGGCGAGATTCGTGACCTGGAAACGGCACAGATCGC
CAACAAGGCTGCACTCACCGGACACCTGATGCTCTCCACTCTTCACACCAATGATGCGATCAGCTCCGTAACACGATTGA
TCGACATGGGTGTCGAACCGTTCCTACTGAGCTCCACACTGCTAGGCGCAATGGCGCAACGACTGGTGCGCGTTATCTGC
GAAGAGTGCAAAGAGGTTGATACCATCGATACGAAACTGCGTCAGACACTCGGCGTTTCCGATGATGAGATCTTCTATCG
TGGAAGAGGATGCCCCACATGTAGCCAGAGCGGCTACCGTGGCCGAGTCGCCGTCTGTGAAATCTTCACCGTAACGCCAG
AGATCCAACAGCTGATCCACGCCAATAAAGGTGAGCAGGCAATCAGAGAGGCGCTTCACAAGGCGGGAATGACAACACTC
TTTGAAGATGCGCTTGAGCTAGCCAGAGAGGGTCGTACCACCCTGGAAGAGGCCTACAAAGTCCGAACCGTCGGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (2-158)

3D structure

• AlphaFold DB: A0A6N0HW74

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	49.615	98.113	0.487
pilB/pilB1	Synechocystis sp. PCC 6803	46.324	100	0.475
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	46.415	100	0.464
pilB	Legionella pneumophila strain ERS1305867	45.283	100	0.453
pilB	Vibrio cholerae strain A1552	44.656	98.868	0.442
pilF	Neisseria gonorrhoeae MS11	43.893	98.868	0.434
ctsE	Campylobacter jejuni subsp. jejuni 81-176	43.462	98.113	0.426
pilB	Acinetobacter baumannii D1279779	41.353	100	0.415
pilB	Acinetobacter baylyi ADP1	40.602	100	0.408
pilB	Vibrio parahaemolyticus RIMD 2210633	41.221	98.868	0.408
pilB	Vibrio campbellii strain DS40M4	40.458	98.868	0.4
pilB	Haemophilus influenzae 86-028NP	41.004	90.189	0.37
pilB	Glaesserella parasuis strain SC1401	45.498	79.623	0.362
pilT	Acinetobacter baylyi ADP1	35.955	100	0.362