Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HUE57_RS04805 Genome accession   NZ_CP054491
Coordinates   854601..855680 (-) Length   359 a.a.
NCBI ID   WP_174672813.1    Uniprot ID   A0A6N0HU24
Organism   Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849601..860680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUE57_RS04780 (HUE57_04780) - 850708..850962 (-) 255 WP_078482045.1 WGR domain-containing protein -
  HUE57_RS04785 (HUE57_04785) - 851089..852240 (-) 1152 WP_078482046.1 deoxyguanosinetriphosphate triphosphohydrolase -
  HUE57_RS04790 (HUE57_04790) aroB 852245..853345 (-) 1101 WP_078482047.1 3-dehydroquinate synthase -
  HUE57_RS04795 (HUE57_04795) - 853410..853934 (-) 525 Protein_982 shikimate kinase -
  HUE57_RS04800 (HUE57_04800) - 854025..854514 (-) 490 Protein_983 type II and III secretion system protein -
  HUE57_RS04805 (HUE57_04805) pilQ 854601..855680 (-) 1080 WP_174672813.1 type IV pilus secretin PilQ Machinery gene
  HUE57_RS04810 (HUE57_04810) - 855655..856206 (-) 552 WP_174672814.1 AMIN domain-containing protein -
  HUE57_RS04815 (HUE57_04815) - 856223..856762 (-) 540 WP_174672815.1 pilus assembly protein PilP -
  HUE57_RS04820 (HUE57_04820) - 856759..857339 (-) 581 Protein_987 type 4a pilus biogenesis protein PilO -
  HUE57_RS20170 (HUE57_04825) - 857343..857480 (-) 138 WP_420885708.1 hypothetical protein -
  HUE57_RS04830 (HUE57_04830) comM 857890..858957 (-) 1068 WP_201790704.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39112.18 Da        Isoelectric Point: 4.4817

>NTDB_id=452866 HUE57_RS04805 WP_174672813.1 854601..855680(-) (pilQ) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
MSDEIESVDFRRGDNGEGKIVIKLSNPSTPIDMHLEGGKVVVDFIENNVPEQLQQRLDVVDFATPVKFIETINLRDNARL
SVTTVGDYEHSGYQTDNTYTVEFRPLSEEEIATKREKEFKGERLSLNFQNIEVRAILQLIADFTGLNIVVSDSVRGNLTL
RLQNVPWDQALDIILKSKGLDMRQTNNVVMVAPAAEIAAREKLELEAAQQITELAPLRTEFIQINYAKASDISTLLKSDS
KNLLSGRGSVSVDARTNILLVQETSDKLAEIRGVIAKLDIPVRQVMIESRIVVASNDFSKALGTKLGYSGVVSDDIGGNN
TLAVTGSGAGADSMVGSGESIPGNGVSPLYLSQTSTIET

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=452866 HUE57_RS04805 WP_174672813.1 854601..855680(-) (pilQ) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
GTGAGCGACGAAATAGAGAGCGTTGATTTTCGGAGGGGTGATAACGGCGAAGGGAAAATAGTAATTAAACTTTCGAATCC
ATCCACGCCCATCGACATGCATCTTGAAGGTGGAAAGGTGGTCGTTGACTTCATTGAAAACAACGTACCCGAACAATTAC
AACAACGTCTTGATGTGGTGGACTTTGCCACCCCCGTGAAGTTCATAGAAACAATCAATCTTCGCGACAACGCCCGCCTT
TCCGTCACCACCGTAGGTGACTATGAACATTCCGGCTACCAAACTGATAACACATATACGGTTGAATTCCGCCCTCTAAG
TGAAGAGGAGATCGCAACAAAACGTGAAAAGGAGTTCAAGGGCGAACGGCTTTCACTAAACTTCCAAAACATTGAAGTAC
GTGCAATCTTGCAACTAATAGCCGACTTTACTGGGTTGAACATCGTCGTTAGCGATTCCGTACGTGGAAACCTGACGCTA
CGCCTTCAGAATGTCCCATGGGACCAAGCATTAGACATTATACTTAAGAGCAAAGGCCTAGACATGCGCCAGACAAACAA
TGTCGTTATGGTCGCGCCAGCTGCTGAAATTGCCGCACGTGAAAAACTAGAGCTCGAAGCTGCACAACAGATAACTGAGC
TTGCCCCGCTGCGCACAGAGTTTATTCAAATCAACTATGCAAAAGCATCCGACATTTCAACCCTACTTAAATCAGACTCT
AAGAATCTTTTATCTGGAAGAGGTAGCGTCAGCGTTGATGCAAGGACAAACATCCTTCTTGTGCAAGAAACGTCAGATAA
GCTAGCCGAAATACGCGGCGTTATTGCCAAGCTCGACATTCCTGTAAGACAGGTAATGATCGAATCAAGAATTGTTGTGG
CAAGCAATGACTTCTCAAAAGCCCTCGGCACCAAACTTGGTTACTCAGGCGTCGTAAGCGATGACATCGGCGGCAATAAC
ACACTAGCTGTTACCGGCTCAGGAGCGGGCGCTGACTCCATGGTCGGTTCAGGAGAGTCAATCCCAGGTAACGGTGTTTC
ACCCCTGTATCTGTCCCAAACCTCAACAATAGAAACATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(220-284)

Secretin and TonB N terminus short domain (STN)

(146-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0HU24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

46.649

100

0.485
  comQ Acinetobacter baylyi ADP1

44.798

96.379

0.432
  pilQ Acinetobacter baumannii strain A118

42.254

98.886

0.418
  pilQ Acinetobacter baumannii D1279779

42.254

98.886

0.418
  pilQ Legionella pneumophila strain Lp02

45.37

90.251

0.409
  pilQ Legionella pneumophila strain ERS1305867

45.37

90.251

0.409
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.615

89.694

0.373
  pilQ Vibrio cholerae strain A1552

41.615

89.694

0.373
  pilQ Vibrio campbellii strain DS40M4

40.06

92.479

0.37