Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HUF19_RS04150 Genome accession   NZ_CP054475
Coordinates   942438..943664 (-) Length   408 a.a.
NCBI ID   WP_260998628.1    Uniprot ID   -
Organism   Thalassolituus hydrocarboniclasticus strain alknpb1M-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 937438..948664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUF19_RS04125 (HUF19_04120) - 939120..939656 (+) 537 WP_260998624.1 PadR family transcriptional regulator -
  HUF19_RS04130 (HUF19_04125) - 939675..940631 (+) 957 WP_260998625.1 DUF808 domain-containing protein -
  HUF19_RS04135 (HUF19_04130) yacG 940688..940888 (-) 201 WP_145469683.1 DNA gyrase inhibitor YacG -
  HUF19_RS04140 (HUF19_04135) coaE 940947..941543 (-) 597 WP_260998626.1 dephospho-CoA kinase -
  HUF19_RS04145 (HUF19_04140) pilD 941560..942438 (-) 879 WP_260998627.1 A24 family peptidase Machinery gene
  HUF19_RS04150 (HUF19_04145) pilC 942438..943664 (-) 1227 WP_260998628.1 type II secretion system F family protein Machinery gene
  HUF19_RS04155 (HUF19_04150) pilB 943712..945409 (-) 1698 WP_260998629.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUF19_RS04160 (HUF19_04155) - 946058..946183 (-) 126 Protein_818 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HUF19_RS04165 (HUF19_04160) - 946394..947038 (-) 645 WP_260998630.1 hypothetical protein -
  HUF19_RS04170 (HUF19_04165) nadC 947073..947936 (-) 864 WP_260998631.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44344.98 Da        Isoelectric Point: 9.5999

>NTDB_id=452706 HUF19_RS04150 WP_260998628.1 942438..943664(-) (pilC) [Thalassolituus hydrocarboniclasticus strain alknpb1M-1]
MAKQIKNSAFAWEGINRRGQTIKGEMSGQNAAMVKAQLRKQGVNPLKVRKVAQPLLGMGGLGKKKIKSSDITFFTRQMAT
MIKAGVPLVQSFDIVADGIDNPSMKELILGLRDTVSAGNDFASALKQHPEYFDELTCNLVESGEQAGALEAMLEKVANYK
EKTEILKAKIKKAMTYPLATLAVAFVVTAILLIKVVPTFDSMFRSFGSQLPAPTQFVVYLSEQAQAYYLHVLGLLAVAGA
FFSQAMKRSPAFRNNFERLTLKLPVFGDLIRKAAVARYARVLSTTFAAGVPLVEALESVAGAVGNVVYREAVLKIRDEVS
SGQQMHFAMRSTGVFPNMVVQMTSIGEESGALDAMLDKAATYFEDEVDNAVDNMTALLEPMIMAFLGVVIGGLIIAMYLP
IFEMGKAI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=452706 HUF19_RS04150 WP_260998628.1 942438..943664(-) (pilC) [Thalassolituus hydrocarboniclasticus strain alknpb1M-1]
ATGGCTAAGCAAATAAAGAACAGCGCCTTTGCCTGGGAAGGCATCAATCGCCGCGGCCAGACAATTAAGGGCGAAATGAG
CGGCCAGAATGCCGCAATGGTCAAGGCGCAGCTGCGTAAGCAGGGTGTTAACCCGCTGAAGGTACGCAAAGTTGCGCAAC
CTTTACTGGGAATGGGTGGGCTGGGCAAGAAAAAGATTAAATCGTCCGATATCACCTTTTTTACCCGGCAGATGGCGACC
ATGATCAAAGCCGGTGTTCCTCTGGTGCAGTCCTTTGATATCGTCGCCGATGGTATTGATAACCCATCGATGAAAGAGCT
GATTCTGGGCCTGCGCGATACCGTATCTGCGGGTAATGACTTTGCCTCTGCACTGAAGCAGCACCCTGAGTACTTTGATG
AGCTTACCTGCAATCTGGTCGAGTCCGGCGAACAGGCCGGTGCCCTTGAAGCCATGCTGGAAAAAGTGGCTAACTACAAA
GAAAAAACCGAGATTCTGAAGGCCAAAATCAAAAAGGCGATGACCTACCCCCTTGCCACACTCGCTGTGGCTTTTGTGGT
AACCGCTATCCTGTTGATTAAAGTAGTACCCACCTTTGACTCTATGTTCCGCAGCTTTGGTTCGCAGCTGCCGGCACCAA
CACAGTTTGTGGTTTATCTGTCTGAACAGGCTCAGGCCTATTACCTGCATGTACTGGGCCTTCTGGCCGTTGCCGGTGCT
TTTTTCTCACAGGCAATGAAACGCTCACCGGCTTTCCGCAATAACTTTGAGCGCCTCACCCTTAAGCTTCCCGTGTTCGG
CGATCTTATCCGCAAGGCGGCGGTTGCCCGTTATGCCCGGGTTCTGTCGACGACCTTTGCTGCCGGTGTGCCCTTAGTAG
AAGCGTTGGAGTCGGTTGCCGGTGCGGTAGGCAATGTTGTTTACCGCGAAGCCGTACTGAAAATCCGCGATGAAGTATCC
TCTGGCCAGCAGATGCACTTTGCGATGCGTTCCACCGGTGTGTTTCCCAATATGGTGGTACAGATGACCAGTATCGGTGA
GGAATCCGGTGCCCTTGATGCCATGCTGGATAAAGCCGCCACTTATTTCGAAGATGAAGTGGATAACGCCGTCGACAATA
TGACAGCCCTGCTTGAGCCGATGATTATGGCGTTCCTCGGTGTGGTAATCGGTGGTCTGATTATCGCCATGTACCTGCCA
ATCTTCGAGATGGGTAAAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

63.3

99.51

0.63
  pilC Acinetobacter baumannii D1279779

57.644

97.794

0.564
  pilC Acinetobacter baylyi ADP1

56.14

97.794

0.549
  pilC Legionella pneumophila strain ERS1305867

52.826

99.755

0.527
  pilG Neisseria gonorrhoeae MS11

44.059

99.02

0.436
  pilG Neisseria meningitidis 44/76-A

44.059

99.02

0.436
  pilC Vibrio cholerae strain A1552

41.266

96.814

0.4
  pilC Vibrio campbellii strain DS40M4

39.461

100

0.395