Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUF19_RS04155 Genome accession   NZ_CP054475
Coordinates   943712..945409 (-) Length   565 a.a.
NCBI ID   WP_260998629.1    Uniprot ID   -
Organism   Thalassolituus hydrocarboniclasticus strain alknpb1M-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 938712..950409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUF19_RS04125 (HUF19_04120) - 939120..939656 (+) 537 WP_260998624.1 PadR family transcriptional regulator -
  HUF19_RS04130 (HUF19_04125) - 939675..940631 (+) 957 WP_260998625.1 DUF808 domain-containing protein -
  HUF19_RS04135 (HUF19_04130) yacG 940688..940888 (-) 201 WP_145469683.1 DNA gyrase inhibitor YacG -
  HUF19_RS04140 (HUF19_04135) coaE 940947..941543 (-) 597 WP_260998626.1 dephospho-CoA kinase -
  HUF19_RS04145 (HUF19_04140) pilD 941560..942438 (-) 879 WP_260998627.1 A24 family peptidase Machinery gene
  HUF19_RS04150 (HUF19_04145) pilC 942438..943664 (-) 1227 WP_260998628.1 type II secretion system F family protein Machinery gene
  HUF19_RS04155 (HUF19_04150) pilB 943712..945409 (-) 1698 WP_260998629.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUF19_RS04160 (HUF19_04155) - 946058..946183 (-) 126 Protein_818 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HUF19_RS04165 (HUF19_04160) - 946394..947038 (-) 645 WP_260998630.1 hypothetical protein -
  HUF19_RS04170 (HUF19_04165) nadC 947073..947936 (-) 864 WP_260998631.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUF19_RS04175 (HUF19_04170) - 947911..949293 (-) 1383 WP_260998632.1 sodium:alanine symporter family protein -
  HUF19_RS04180 (HUF19_04175) ampD 949475..950032 (+) 558 WP_260998633.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61528.44 Da        Isoelectric Point: 4.9015

>NTDB_id=452707 HUF19_RS04155 WP_260998629.1 943712..945409(-) (pilB) [Thalassolituus hydrocarboniclasticus strain alknpb1M-1]
MAVLTGLPRRIVEQNLVNSQQAQEIINKAKQDKISFVAAAVASGYIKSRAIALLASEEFGLPLLDLSAFDFELMPKELVS
RELIEKHRVLPLFQRGNRLFVAQSDPSAMLAIDEIKFNTGMTVEAVLAEEDKLGSAVDKFLAANDNSFGDLDGDLDDVDL
EDPDARDKAQEAEKDSSGDDAPIVRFVNKMLLDAIKGGASDIHFEPYEKRYRVRYRTDGMLHEVASPPVNLAGKIAARLK
VMSQMDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDMLGYEADQKALYLDALAKPQGM
ILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNARVGLDFAAALRSFLRQDPDVVMVGEIRDLD
TASIAIKAAQTGHLVLSTLHTNSAAETLTRLANMGVPSFNIATSVSLIIAQRLARRLCASCKAPAEIPKELLLEAGFTEE
QLQTAEIYKPVGCEQCKGGYKGRVGIYEVVRITDAISRIIMEGGNSIEISDAARKEGFNDLRTSALQKVAQGVTSLEEAN
RVTTD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=452707 HUF19_RS04155 WP_260998629.1 943712..945409(-) (pilB) [Thalassolituus hydrocarboniclasticus strain alknpb1M-1]
ATGGCCGTTTTGACCGGCTTACCACGCCGTATCGTTGAGCAGAATCTGGTGAACAGCCAGCAGGCTCAGGAAATCATCAA
TAAGGCAAAGCAGGACAAAATCAGTTTTGTTGCCGCCGCCGTTGCATCGGGTTACATAAAATCACGTGCCATTGCACTGC
TGGCTTCTGAAGAGTTCGGCCTCCCCCTGCTGGATCTCAGTGCCTTTGATTTTGAGCTGATGCCCAAAGAACTGGTCAGC
CGTGAACTGATCGAAAAGCACCGGGTTCTGCCATTGTTTCAGCGCGGAAACCGTCTGTTTGTCGCGCAAAGCGACCCATC
CGCCATGCTGGCGATCGACGAAATCAAGTTTAATACCGGCATGACCGTCGAGGCGGTACTGGCTGAAGAAGATAAACTCG
GCAGTGCTGTCGATAAATTCCTGGCCGCTAACGACAACAGCTTTGGTGATCTGGACGGTGATCTCGACGATGTGGATCTG
GAAGACCCGGACGCCCGGGATAAAGCCCAAGAAGCCGAGAAGGATTCCTCCGGCGACGACGCTCCGATCGTACGTTTTGT
TAATAAAATGCTGCTCGATGCGATTAAAGGCGGCGCTTCCGATATTCACTTTGAGCCTTACGAAAAGCGCTACCGGGTAC
GTTACCGTACCGATGGCATGCTGCATGAAGTTGCCAGCCCGCCAGTCAACCTTGCCGGCAAGATTGCTGCGCGCTTAAAG
GTGATGTCACAGATGGATATCTCTGAGCGGCGCGTTCCCCAGGATGGCCGTATCAAGATGAAGCTGTCCAAAACCAAGGC
CATCGATTTCCGCGTCAATACCCTGCCAACCCTGTGGGGTGAAAAGATCGTACTGCGTATTCTCGATCCTTCTTCAGCCA
AGCTGGGGATTGATATGCTCGGTTATGAAGCCGATCAGAAAGCCCTGTATCTGGATGCCCTGGCCAAGCCGCAGGGCATG
ATTCTGGTTACCGGCCCGACCGGTTCCGGTAAAACGGTATCCCTGTATACCGGTCTGAATATCCTTAACGTGGAAGGCAC
CAATATTTCCACCGCGGAAGACCCGGTAGAAATTAACCTCGAAGGTATTAACCAGGTAAACGTAAACGCCCGTGTCGGTC
TCGACTTCGCAGCAGCTCTGCGCTCCTTCCTGCGTCAGGACCCGGATGTGGTAATGGTGGGGGAAATCCGGGATCTGGAT
ACCGCCTCTATCGCCATCAAAGCGGCCCAGACCGGTCACTTAGTGCTTTCCACCCTGCACACCAACAGTGCTGCCGAAAC
CCTGACCCGTCTGGCCAATATGGGTGTGCCATCGTTCAATATTGCCACCTCGGTCAGTCTGATTATCGCCCAGCGTCTGG
CGCGGCGTTTATGCGCAAGCTGCAAAGCCCCGGCAGAGATTCCTAAAGAGCTGCTGCTGGAGGCCGGATTCACCGAAGAA
CAGCTGCAAACCGCTGAGATTTATAAGCCGGTTGGCTGTGAGCAATGTAAAGGCGGTTATAAAGGCCGTGTCGGTATTTA
TGAAGTAGTTAGAATAACCGATGCTATCTCCCGTATTATCATGGAGGGCGGCAACTCGATTGAGATTTCTGATGCAGCGC
GCAAAGAGGGTTTTAACGATTTGCGTACATCGGCGCTGCAAAAGGTCGCCCAAGGGGTTACCAGCCTGGAAGAAGCCAAC
CGGGTAACCACCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

63.186

100

0.632
  pilB Acinetobacter baylyi ADP1

61.239

100

0.612
  pilB Legionella pneumophila strain ERS1305867

54.401

100

0.547
  pilB Vibrio cholerae strain A1552

51.318

100

0.517
  pilB Vibrio parahaemolyticus RIMD 2210633

51.706

98.584

0.51
  pilB Vibrio campbellii strain DS40M4

50

100

0.501
  pilF Neisseria gonorrhoeae MS11

50.088

100

0.501
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.7

94.513

0.375
  pilF Thermus thermophilus HB27

35.902

100

0.363