Detailed information NTG0045263

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL3_RS13120
• Genome accession: NC_017190
• Coordinates: 2587947..2588561 (-)
• Length: 204 a.a.
• NCBI ID: WP_014470701.1
• Uniprot ID: A0AAP7N5G8
• Organism: Bacillus amyloliquefaciens LL3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2582947..2593561

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL3_RS13095 (LL3_02750)	rpsT	2583247..2583513 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LL3_RS13100 (LL3_02751)	holA	2583536..2584571 (-)	1036	Protein_2639	DNA polymerase III subunit delta	-
LL3_RS21110	-	2584611..2584765 (-)	155	Protein_2640	hypothetical protein	-
LL3_RS20700 (LL3_02752)	-	2584806..2584940 (+)	135	WP_003152870.1	YqzM family protein	-
LL3_RS13110 (LL3_02753)	-	2584959..2587311 (-)	2353	Protein_2642	DNA internalization-related competence protein ComEC/Rec2	-
LL3_RS13115 (LL3_02754)	-	2587312..2587881 (-)	570	WP_014470700.1	ComE operon protein 2	-
LL3_RS13120 (LL3_02755)	comEA	2587947..2588561 (-)	615	WP_014470701.1	helix-hairpin-helix domain-containing protein	Machinery gene
LL3_RS13125 (LL3_02756)	comER	2588621..2589442 (+)	822	WP_014470702.1	late competence protein ComER	-
LL3_RS13130 (LL3_02757)	-	2589511..2590248 (-)	738	WP_014470703.1	class I SAM-dependent methyltransferase	-
LL3_RS13135 (LL3_02758)	rsfS	2590245..2590601 (-)	357	WP_014470704.1	ribosome silencing factor	-
LL3_RS13140 (LL3_02759)	yqeK	2590618..2591178 (-)	561	WP_014470705.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LL3_RS13145 (LL3_02760)	-	2591168..2591737 (-)	570	WP_014470706.1	nicotinate-nucleotide adenylyltransferase	-
LL3_RS13150 (LL3_02761)	yhbY	2591748..2592038 (-)	291	WP_014470707.1	ribosome assembly RNA-binding protein YhbY	-
LL3_RS13155 (LL3_02762)	aroE	2592032..2592874 (-)	843	WP_014470708.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22180.40 Da
• Isoelectric Point: 5.4688

>NTDB_id=45263 LL3_RS13120 WP_014470701.1 2587947..2588561(-) (comEA) [Bacillus amyloliquefaciens LL3]
MTKVMMTERQRQYLKILGVCLAAGAVLICILLFRSEKEEPVKQQSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTIVYIPSEGEEANPPKTAAGEKENASVNINTASLDELQAIPGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=45263 LL3_RS13120 WP_014470701.1 2587947..2588561(-) (comEA) [Bacillus amyloliquefaciens LL3]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
GATCTGCATTTTGCTGTTTCGTTCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCGC
CCGTCACGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTTTAT
GAAATGAAGGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATCGTTTATATCCCGAGTGAAGGCGAAGAGGCGAATCCCCCGAAAACCG
CAGCAGGTGAAAAGGAGAATGCTTCTGTCAATATCAATACGGCATCTCTTGACGAACTTCAGGCGATTCCCGGTGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAATGGGCGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.436	95.588	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.945	100	0.382
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.945	100	0.382
comEA/celA/cilE	Streptococcus pneumoniae D39	35.945	100	0.382
comEA/celA/cilE	Streptococcus pneumoniae R6	35.945	100	0.382
comEA	Staphylococcus aureus MW2	35.294	100	0.382
comEA	Latilactobacillus sakei subsp. sakei 23K	33.333	100	0.382
comEA	Staphylococcus aureus N315	34.842	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.484	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.023	100	0.373