Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   I5367_RS07885 Genome accession   NZ_CP065539
Coordinates   1561277..1563220 (+) Length   647 a.a.
NCBI ID   WP_012117534.1    Uniprot ID   A7Z4J7
Organism   Bacillus amyloliquefaciens strain DGL1     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1556277..1568220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5367_RS07860 (I5367_07860) def 1556651..1557133 (+) 483 WP_012117530.1 peptide deformylase -
  I5367_RS07865 (I5367_07865) fmt 1557138..1558091 (+) 954 WP_012117531.1 methionyl-tRNA formyltransferase -
  I5367_RS07870 (I5367_07870) rsmB 1558078..1559421 (+) 1344 WP_012117532.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  I5367_RS07875 (I5367_07875) rlmN 1559425..1560516 (+) 1092 WP_003154339.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I5367_RS07880 (I5367_07880) - 1560522..1561283 (+) 762 WP_041482191.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  I5367_RS07885 (I5367_07885) stkP 1561277..1563220 (+) 1944 WP_012117534.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  I5367_RS07890 (I5367_07890) rsgA 1563235..1564125 (+) 891 WP_012117535.1 ribosome small subunit-dependent GTPase A -
  I5367_RS07895 (I5367_07895) rpe 1564127..1564777 (+) 651 WP_012117536.1 ribulose-phosphate 3-epimerase -
  I5367_RS07900 (I5367_07900) - 1564846..1565490 (+) 645 WP_012117537.1 thiamine diphosphokinase -
  I5367_RS07905 (I5367_07905) spoVM 1565577..1565657 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  I5367_RS07910 (I5367_07910) rpmB 1565735..1565923 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  I5367_RS07915 (I5367_07915) - 1566182..1566544 (+) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71694.28 Da        Isoelectric Point: 5.0248

>NTDB_id=449984 I5367_RS07885 WP_012117534.1 1561277..1563220(+) (stkP) [Bacillus amyloliquefaciens strain DGL1]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQNPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPEGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=449984 I5367_RS07885 WP_012117534.1 1561277..1563220(+) (stkP) [Bacillus amyloliquefaciens strain DGL1]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCCGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAAATCCCTTTTGACGGAGAAT
CAGCCGTCAGTATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTTATTACTGACGGCGCAAAAGCCGCGCCTCAGGCGGAAACGCCGGGGGAGACGGATGATAAGGAACAGCAGCCCGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCGGCTGTTCTTGCGGTCAC
CGTGTTTCCGTCTTTATTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTCCGGGCGGATCCTGATATAACGGACATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGACGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAGAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGAACCCGTCAGCCGGCACCGACATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCGGAAGGACAAG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCATTTAAAATAAAAGAGCCGACTGAAAAAACAA
TCGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGATTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z4J7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.799

95.209

0.369
  stkP Streptococcus pneumoniae D39

38.636

95.209

0.368
  stkP Streptococcus pneumoniae R6

38.636

95.209

0.368
  stkP Streptococcus pneumoniae Rx1

38.474

95.209

0.366
  pknB Streptococcus mutans UA159

40.838

88.563

0.362