Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPY11_RS02475 Genome accession   NZ_CP053820
Coordinates   511637..512062 (-) Length   141 a.a.
NCBI ID   WP_142609993.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA10G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506637..517062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY11_RS02455 (HPY11_02455) coaE 507138..507746 (-) 609 WP_162810836.1 dephospho-CoA kinase -
  HPY11_RS02460 (HPY11_02460) pilD 507743..508618 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  HPY11_RS02465 (HPY11_02465) pilC 508676..509902 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY11_RS02470 (HPY11_02470) pilB 509945..511633 (-) 1689 WP_175245917.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY11_RS02475 (HPY11_02475) pilA 511637..512062 (-) 426 WP_142609993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY11_RS02480 (HPY11_02480) nadC 512307..513197 (-) 891 WP_175245918.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY11_RS02485 (HPY11_02485) ampD 513493..514038 (+) 546 WP_175245919.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY11_RS02490 (HPY11_02490) fldB 514209..514727 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY11_RS02495 (HPY11_02495) xerD 514865..515773 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY11_RS02500 (HPY11_02500) dsbC 515790..516542 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14586.67 Da        Isoelectric Point: 7.8085

>NTDB_id=447836 HPY11_RS02475 WP_142609993.1 511637..512062(-) (pilA) [Vibrio cholerae strain SA10G]
MKAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPAYKDYVTKSELASGFATIKSVITPAELYFQENGKISASASTVDVLGV
SSGANTLGELTITADNTIQFAHDAGAALGAKFVYTRSSGGWSCTYTQPSAVSAAAPKGCTP

Nucleotide


Download         Length: 426 bp        

>NTDB_id=447836 HPY11_RS02475 WP_142609993.1 511637..512062(-) (pilA) [Vibrio cholerae strain SA10G]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTAATGATTGTGGTAGCGATTATTGGTGTGTT
GGCAGCGATTGCTGTTCCTGCTTATAAGGATTATGTAACCAAGAGCGAATTGGCATCTGGTTTCGCTACTATCAAGTCCG
TGATAACTCCAGCTGAACTTTATTTCCAAGAAAATGGAAAAATAAGTGCAAGCGCAAGCACTGTTGATGTTCTCGGTGTA
TCTTCTGGAGCAAATACTTTAGGTGAACTTACTATAACCGCTGACAATACAATTCAATTTGCTCATGATGCTGGTGCTGC
ATTAGGTGCAAAATTTGTTTACACAAGAAGCTCTGGAGGTTGGAGTTGCACTTACACACAACCATCAGCAGTTAGTGCAG
CAGCTCCTAAGGGCTGTACACCATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.414

100

0.539
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.871

100

0.482
  pilA Vibrio cholerae strain A1552

43.871

100

0.482
  pilA Vibrio cholerae C6706

43.871

100

0.482
  pilA Pseudomonas aeruginosa PAK

39.735

100

0.426
  pilA Acinetobacter baumannii strain A118

39.189

100

0.411
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.695

92.908

0.369
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.681

97.872

0.369