Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPY11_RS02470 Genome accession   NZ_CP053820
Coordinates   509945..511633 (-) Length   562 a.a.
NCBI ID   WP_175245917.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA10G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504945..516633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY11_RS02445 (HPY11_02445) yacG 505995..506192 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY11_RS02450 (HPY11_02450) zapD 506366..507106 (-) 741 WP_000207194.1 cell division protein ZapD -
  HPY11_RS02455 (HPY11_02455) coaE 507138..507746 (-) 609 WP_162810836.1 dephospho-CoA kinase -
  HPY11_RS02460 (HPY11_02460) pilD 507743..508618 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  HPY11_RS02465 (HPY11_02465) pilC 508676..509902 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY11_RS02470 (HPY11_02470) pilB 509945..511633 (-) 1689 WP_175245917.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY11_RS02475 (HPY11_02475) pilA 511637..512062 (-) 426 WP_142609993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY11_RS02480 (HPY11_02480) nadC 512307..513197 (-) 891 WP_175245918.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY11_RS02485 (HPY11_02485) ampD 513493..514038 (+) 546 WP_175245919.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY11_RS02490 (HPY11_02490) fldB 514209..514727 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY11_RS02495 (HPY11_02495) xerD 514865..515773 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY11_RS02500 (HPY11_02500) dsbC 515790..516542 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62051.11 Da        Isoelectric Point: 5.9427

>NTDB_id=447835 HPY11_RS02470 WP_175245917.1 509945..511633(-) (pilB) [Vibrio cholerae strain SA10G]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLSLGIFPAQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNNELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=447835 HPY11_RS02470 WP_175245917.1 509945..511633(-) (pilB) [Vibrio cholerae strain SA10G]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGAACTTCGGTGCCCGAGGCGCTGCTCAGTTTGGGCATTTTCCCCGCCCAAGAACTGACCGAGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGATCTTAGCCGTTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTTGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCGG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCTGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGCGTTTCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTGAAAAGACCGCAAGGGATGATT
TTGATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCACTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTCAAAGCCGCGCAAACCGGTCACTTAGTGCTTTCCACTCTGCACACCAACTCGGCTGCCGAAACCGT
GATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAATCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTACGCCCTTCTGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATAACGAGTTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.288

100

0.993
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.843

100

0.738
  pilB Legionella pneumophila strain ERS1305867

51.81

93.416

0.484
  pilB Acinetobacter baylyi ADP1

49.812

94.662

0.472
  pilB Acinetobacter baumannii D1279779

50.29

91.993

0.463
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37

100

0.395
  pilF Thermus thermophilus HB27

40.111

96.263

0.386