Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RGE_RS20785 Genome accession   NC_017075
Coordinates   4571896..4573128 (+) Length   410 a.a.
NCBI ID   WP_014430447.1    Uniprot ID   I0HX61
Organism   Rubrivivax gelatinosus IL144     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4566896..4578128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE_RS20770 (RGE_42590) - 4567820..4568830 (+) 1011 WP_014430444.1 sulfotransferase -
  RGE_RS20775 (RGE_42600) chrA 4568830..4570047 (+) 1218 WP_014430445.1 chromate efflux transporter -
  RGE_RS20780 (RGE_42610) pilB 4570152..4571876 (+) 1725 WP_014430446.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RGE_RS20785 (RGE_42620) pilG 4571896..4573128 (+) 1233 WP_014430447.1 type II secretion system F family protein Machinery gene
  RGE_RS20790 (RGE_42630) - 4573125..4574075 (+) 951 WP_014430448.1 A24 family peptidase -
  RGE_RS20795 (RGE_42640) coaE 4574057..4574683 (+) 627 WP_014430449.1 dephospho-CoA kinase -
  RGE_RS20800 (RGE_42650) zapD 4574711..4575466 (+) 756 WP_014430450.1 cell division protein ZapD -
  RGE_RS20805 (RGE_42660) - 4575490..4575690 (+) 201 WP_014430451.1 DNA gyrase inhibitor YacG -
  RGE_RS20810 (RGE_42670) nifB 4575882..4577456 (+) 1575 WP_014430452.1 nitrogenase cofactor biosynthesis protein NifB -
  RGE_RS20815 (RGE_42680) - 4577490..4577684 (+) 195 WP_014430453.1 4Fe-4S binding protein -
  RGE_RS20820 (RGE_42690) - 4577687..4578073 (+) 387 WP_014430454.1 iron-sulfur cluster assembly accessory protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44795.87 Da        Isoelectric Point: 10.0005

>NTDB_id=44741 RGE_RS20785 WP_014430447.1 4571896..4573128(+) (pilG) [Rubrivivax gelatinosus IL144]
MATAASAARAKSAAKEQIFEWEGKDKNGKLVRGEIRAGGEAVVNASLRRQGILVTKVKKRRTSGGRSIKQKDIAIFTRQL
ATMMKAGVPLLQAFDIVARGASNPRMTKLLTDIRSDVETGTSLSAAFRKHPMYFDALYCNLIEAGEAGGILEALLERLAI
YQEKTMALKQKIKSALMYPVAVIVVAFIVLTVIMIFVIPAFKEVFRSFGADLPAPTLFVIALSEFFVSWWWVIFGLIFGG
TYFFLQSWKRSEKMQMVMDRLLLRLPIFGTLIEKSVIARWTRTLATMFAAGVPLVEALDSVGGASGNAVYARATEQIQKD
VSTGSALTTSMTATSVFPTMVLQMSAIGEESGSLDHMLGKAAEFYEEEVDDMVKGLSSLMEPFIIVILGVLIGGIVVSMY
LPIFKLGAVV

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=44741 RGE_RS20785 WP_014430447.1 4571896..4573128(+) (pilG) [Rubrivivax gelatinosus IL144]
ATGGCCACAGCAGCATCCGCCGCGCGCGCCAAGAGCGCCGCCAAGGAACAGATCTTCGAGTGGGAGGGCAAGGACAAGAA
CGGCAAGCTCGTGCGCGGCGAGATCCGCGCCGGGGGCGAGGCCGTCGTCAACGCCAGCCTGCGCCGCCAGGGCATCCTGG
TCACCAAGGTCAAGAAGCGCCGCACCAGCGGCGGCCGCTCGATCAAGCAGAAGGACATCGCGATCTTCACGCGCCAGCTG
GCGACGATGATGAAGGCCGGCGTGCCGCTGCTGCAGGCCTTCGACATCGTCGCGCGCGGGGCGTCCAACCCGCGCATGAC
CAAGCTGCTGACGGACATCCGCTCGGACGTCGAGACCGGCACCAGCCTGTCGGCCGCGTTCCGCAAGCACCCGATGTACT
TCGACGCGCTGTACTGCAACCTGATCGAAGCCGGCGAGGCCGGCGGCATCCTGGAAGCGCTGCTCGAGCGCCTGGCGATC
TACCAGGAGAAGACGATGGCGCTGAAGCAGAAGATCAAGTCGGCGCTGATGTACCCGGTGGCGGTCATCGTCGTCGCCTT
CATCGTGCTGACGGTCATCATGATCTTCGTGATTCCGGCGTTCAAGGAGGTCTTCCGCTCCTTCGGCGCCGACCTGCCGG
CGCCGACGCTGTTCGTGATCGCGCTGTCCGAGTTCTTCGTCAGCTGGTGGTGGGTGATCTTCGGCCTGATCTTCGGCGGC
ACCTACTTCTTCCTGCAGTCCTGGAAGCGCTCGGAGAAGATGCAGATGGTCATGGACCGGCTGCTGCTGCGCCTGCCGAT
CTTCGGCACGCTGATCGAGAAGTCGGTGATCGCGCGCTGGACGCGCACGCTGGCGACGATGTTCGCCGCCGGCGTGCCGC
TGGTCGAGGCGCTCGACTCGGTCGGCGGCGCCTCGGGCAACGCCGTCTACGCCCGGGCCACCGAGCAGATCCAGAAGGAC
GTCTCCACCGGCTCGGCGCTGACCACCTCGATGACCGCGACCTCGGTCTTCCCGACGATGGTGCTGCAGATGTCGGCCAT
CGGCGAGGAATCCGGCTCTCTGGACCACATGCTGGGCAAGGCCGCCGAGTTCTACGAGGAAGAGGTCGACGACATGGTCA
AGGGCCTGTCGAGCCTGATGGAGCCCTTCATCATCGTCATCCTGGGCGTGCTGATCGGCGGCATCGTGGTCTCGATGTAC
CTGCCGATCTTCAAGCTGGGCGCCGTCGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I0HX61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

55.138

97.317

0.537
  pilG Neisseria meningitidis 44/76-A

54.887

97.317

0.534
  pilC Pseudomonas stutzeri DSM 10701

53.96

98.537

0.532
  pilC Acinetobacter baylyi ADP1

50

99.024

0.495
  pilC Legionella pneumophila strain ERS1305867

50.253

96.585

0.485
  pilC Acinetobacter baumannii D1279779

48.889

98.78

0.483
  pilC Vibrio campbellii strain DS40M4

42.5

97.561

0.415
  pilC Vibrio cholerae strain A1552

41.457

97.073

0.402
  pilC Thermus thermophilus HB27

39.25

97.561

0.383

Multiple sequence alignment