Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RGE_RS20780 Genome accession   NC_017075
Coordinates   4570152..4571876 (+) Length   574 a.a.
NCBI ID   WP_014430446.1    Uniprot ID   I0HX60
Organism   Rubrivivax gelatinosus IL144     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4565152..4576876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE_RS20760 (RGE_42570) - 4565571..4566122 (+) 552 WP_014430442.1 hypothetical protein -
  RGE_RS24960 (RGE_42580) - 4566187..4567734 (+) 1548 WP_014430443.1 methyl-accepting chemotaxis protein -
  RGE_RS20770 (RGE_42590) - 4567820..4568830 (+) 1011 WP_014430444.1 sulfotransferase -
  RGE_RS20775 (RGE_42600) chrA 4568830..4570047 (+) 1218 WP_014430445.1 chromate efflux transporter -
  RGE_RS20780 (RGE_42610) pilB 4570152..4571876 (+) 1725 WP_014430446.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RGE_RS20785 (RGE_42620) pilG 4571896..4573128 (+) 1233 WP_014430447.1 type II secretion system F family protein Machinery gene
  RGE_RS20790 (RGE_42630) - 4573125..4574075 (+) 951 WP_014430448.1 A24 family peptidase -
  RGE_RS20795 (RGE_42640) coaE 4574057..4574683 (+) 627 WP_014430449.1 dephospho-CoA kinase -
  RGE_RS20800 (RGE_42650) zapD 4574711..4575466 (+) 756 WP_014430450.1 cell division protein ZapD -
  RGE_RS20805 (RGE_42660) - 4575490..4575690 (+) 201 WP_014430451.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62716.00 Da        Isoelectric Point: 5.4804

>NTDB_id=44740 RGE_RS20780 WP_014430446.1 4570152..4571876(+) (pilB) [Rubrivivax gelatinosus IL144]
MPADTTVETQQTALSGVARVLVHAGKLNARTAEDLARSARERKTSFVSAVVAAGALKPDELAHALSGALALPLLDLNAVD
LQKLPRNLLDAKTATQYQVIVLGRRGNRLFIGAADPTDQEAVERIKFATQLTPEWVIVEYDKLMRQLDVQGQSANDVLES
LAGEDFDFDVSDDTTTTAAEAEVSTEVEDAPVVRFLQKMLIDAINARASDLHFEPYEYNYRVRFRIDGELREITQPPIAI
KDKLASRIKVISRMDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKVVIRILDPSSARLGIEALGYEKIEKDRLMA
AIQRPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNIATVEDPAEINLPGINQVNVNDKAGLTFAAALKAFLRQDPDIIM
VGEIRDLETADIAIKAAQTGHLVMSTLHTNDAPTTLTRLLNMGVAPFNIAASVLLITAQRLARRLCEVCKKPADYPREAL
LKAGYTDADLDGSWKPYRAVGCSACNNGYKGRVGIYQVMPITEAIQRVILSEGTALDIAEQAQREGVRDLRQSGLVKVRL
GVTSLEEVITVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=44740 RGE_RS20780 WP_014430446.1 4570152..4571876(+) (pilB) [Rubrivivax gelatinosus IL144]
ATGCCCGCCGACACCACCGTCGAGACACAGCAGACCGCGCTGTCCGGCGTCGCCCGGGTCCTGGTGCACGCCGGCAAGCT
CAACGCGCGCACCGCCGAGGACCTGGCGCGCAGCGCGCGCGAGCGCAAGACCAGCTTCGTCTCGGCCGTCGTCGCCGCCG
GGGCGCTCAAGCCCGACGAGCTGGCGCACGCGCTGTCGGGCGCGCTGGCCCTGCCGCTGCTGGACCTCAACGCGGTCGAC
CTGCAGAAGCTGCCGCGCAACCTGCTCGACGCCAAGACGGCGACGCAGTACCAGGTCATCGTGCTCGGCCGGCGCGGCAA
CCGGCTGTTCATCGGCGCGGCCGACCCCACCGACCAGGAAGCCGTCGAGCGCATCAAGTTCGCCACCCAGCTGACCCCGG
AGTGGGTCATCGTCGAGTACGACAAGCTGATGCGCCAGCTCGACGTCCAGGGCCAGAGCGCCAACGACGTGCTCGAGTCC
CTGGCCGGCGAGGACTTCGACTTCGACGTCAGCGACGACACGACGACGACCGCCGCCGAGGCCGAGGTCTCGACCGAAGT
CGAGGACGCGCCGGTCGTGCGCTTCCTGCAGAAGATGCTGATCGACGCGATCAACGCCCGCGCCTCCGACCTGCACTTCG
AGCCCTACGAATACAACTACCGGGTGCGCTTCCGCATCGACGGCGAGCTGCGCGAGATCACCCAGCCGCCGATCGCCATC
AAGGACAAGCTCGCCTCGCGCATCAAGGTCATCTCGCGCATGGACATCGCCGAGAAGCGGGTCCCGCAGGACGGCCGGAT
GAAGCTGAAGTTCGGCAGCAAGGCGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGGTCGTGATCC
GGATCCTGGACCCTTCGTCGGCGCGCCTGGGCATCGAGGCCCTGGGCTACGAGAAGATCGAGAAGGACCGCCTGATGGCC
GCGATCCAGCGGCCCTACGGCATGATCCTCGTCACCGGCCCGACCGGCAGCGGCAAGACGGTGTCGCTCTACACCTGCCT
GAACATCCTCAACCAGCCGGGCGTGAACATCGCCACCGTCGAGGATCCGGCCGAAATCAACCTGCCGGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGGCCTTCCTGCGCCAGGACCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAGACCGCCGACATCGCGATCAAGGCGGCGCAGACCGGCCACCTGGTGATGAGCACGCT
GCACACCAACGACGCGCCGACGACGCTGACGCGGCTGCTGAACATGGGCGTGGCGCCGTTCAACATCGCCGCCAGCGTGC
TGTTGATCACCGCGCAACGCCTGGCGCGCCGTTTGTGTGAAGTCTGCAAGAAACCGGCGGACTATCCGCGCGAGGCGCTG
CTCAAGGCGGGGTATACCGACGCCGATCTGGACGGAAGCTGGAAGCCGTATCGTGCGGTCGGCTGCTCCGCCTGCAACAA
CGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCACCGAGGCCATCCAGCGCGTCATCCTCTCCGAGGGCA
CCGCGCTGGACATCGCCGAGCAGGCGCAGCGCGAGGGCGTGCGCGACCTGCGCCAGTCCGGTCTGGTGAAGGTGCGCCTG
GGCGTCACCTCGCTGGAAGAAGTCATCACCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I0HX60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.965

98.258

0.54
  pilF Neisseria gonorrhoeae MS11

54.352

98.084

0.533
  pilB Acinetobacter baylyi ADP1

53.873

98.955

0.533
  pilB Legionella pneumophila strain ERS1305867

48.78

100

0.488
  pilB Vibrio cholerae strain A1552

45.406

98.606

0.448
  pilB Vibrio campbellii strain DS40M4

44.779

98.432

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

44.76

98.084

0.439
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.678

92.509

0.376
  pilF Thermus thermophilus HB27

37.5

98.955

0.371

Multiple sequence alignment