Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HPQ68_RS03500 Genome accession   NZ_CP053748
Coordinates   770524..771714 (-) Length   396 a.a.
NCBI ID   WP_304665261.1    Uniprot ID   -
Organism   Massilia sp. erpn     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765524..776714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPQ68_RS03475 (HPQ68_03465) - 766846..767772 (-) 927 WP_255756473.1 methyltransferase domain-containing protein -
  HPQ68_RS03480 (HPQ68_03470) - 767832..768554 (+) 723 WP_255756474.1 ComF family protein -
  HPQ68_RS03485 (HPQ68_03475) trmL 768564..769034 (+) 471 WP_255756475.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  HPQ68_RS03490 (HPQ68_03480) - 769193..770032 (+) 840 WP_255756476.1 transposase -
  HPQ68_RS03495 (HPQ68_03485) rfaE2 770029..770511 (-) 483 WP_176349188.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  HPQ68_RS03500 (HPQ68_03490) pilU 770524..771714 (-) 1191 WP_304665261.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPQ68_RS03505 (HPQ68_03495) - 771818..772759 (+) 942 WP_255756477.1 hydrogen peroxide-inducible genes activator -
  HPQ68_RS03510 (HPQ68_03500) ubiA 772833..773687 (+) 855 WP_255758216.1 4-hydroxybenzoate octaprenyltransferase -
  HPQ68_RS03515 (HPQ68_03505) - 773771..774145 (+) 375 WP_255756478.1 YqjD family protein -
  HPQ68_RS03520 (HPQ68_03510) - 774171..774965 (-) 795 WP_255756479.1 transporter substrate-binding domain-containing protein -
  HPQ68_RS03525 (HPQ68_03515) proC 775070..775906 (-) 837 WP_255756480.1 pyrroline-5-carboxylate reductase -
  HPQ68_RS03530 (HPQ68_03520) - 775903..776631 (-) 729 WP_255756481.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43983.13 Da        Isoelectric Point: 6.1519

>NTDB_id=446878 HPQ68_RS03500 WP_304665261.1 770524..771714(-) (pilU) [Massilia sp. erpn]
MSSIFGHEEAQAYMHKLLKAMHQVGGSDLFISADFPPSIKSQGAMKALSQQRLSGEVTRALACAIMNEKQRQEFESELEC
NFAISLPDVCRFRVNVFVQQQNVAMVIRTIASEIPNFDKLDLPEVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNG
HSAGHIITVEDPVEYVHKNKGCLVTHREVGVDTHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTIAEMIFKGSFQTIKEIMHKS
RELGMCTFDQALYELYNKGYISYEEALRNADSANGLRLQIKLRGDRREPGGAAAAGGAAELSMQMDEEDEEEPGSH

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=446878 HPQ68_RS03500 WP_304665261.1 770524..771714(-) (pilU) [Massilia sp. erpn]
ATGTCGTCGATTTTTGGCCACGAAGAAGCACAGGCATATATGCACAAGCTGCTAAAAGCCATGCACCAGGTGGGCGGTTC
GGACTTGTTCATCTCGGCCGACTTCCCGCCCAGCATCAAATCCCAGGGCGCCATGAAGGCGCTCAGCCAGCAGCGCCTGA
GCGGCGAGGTGACGCGCGCCCTGGCCTGCGCCATCATGAACGAGAAGCAGCGCCAGGAGTTCGAGAGCGAGCTCGAATGC
AACTTCGCCATCTCGCTGCCGGACGTGTGCCGCTTTCGCGTCAACGTCTTCGTGCAGCAGCAGAATGTGGCGATGGTGAT
CCGCACCATCGCCTCCGAAATCCCGAACTTCGACAAGCTTGACCTGCCCGAGGTGCTGAAGGATGTGGTGATGACCAAGC
GCGGCCTGGTGCTGGTGGTGGGCGGCACCGGTTCCGGCAAATCGACCACCTTGGCAGCCATGATCGATTACCGCAACGGC
CACTCGGCCGGCCACATCATCACGGTGGAAGACCCGGTCGAATACGTGCACAAGAACAAGGGCTGCCTGGTGACGCACCG
CGAAGTGGGCGTGGACACGCACAGCTGGCACAACGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAAATCCGCGACACCGAAACCATGGAGCATGCGATCGCCTTCGCCGAAACAGGCCACCTCTGCCTGGGCACCCTGCAC
GCGAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGCAACCAGCTGCTGATGGACCT
GTCCTCGAATCTGCGCGCCATCGTCTCGCAGCGCCTGATCCGCACCGAGGACGGCAAAGGGCGCAAGGCCGCCATTGAAA
TCCTGCTCAACACGCCGACGATTGCCGAGATGATTTTCAAGGGCAGCTTCCAGACCATCAAGGAAATCATGCACAAATCG
CGCGAGCTGGGCATGTGCACTTTCGACCAGGCGCTGTACGAACTCTATAATAAGGGTTACATCTCATACGAAGAAGCGTT
GCGCAACGCCGACTCGGCCAACGGCCTGCGTCTGCAGATCAAGCTGCGCGGCGACCGCAGGGAGCCAGGCGGCGCTGCCG
CTGCCGGCGGCGCGGCCGAACTGAGCATGCAGATGGATGAGGAAGACGAAGAAGAACCCGGCTCACACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(88-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.728

95.707

0.543
  pilU Acinetobacter baylyi ADP1

54.52

89.394

0.487
  pilU Vibrio cholerae strain A1552

52.125

89.141

0.465
  pilT Pseudomonas aeruginosa PAK

44.478

84.596

0.376
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.713

84.343

0.369
  pilT Acinetobacter baylyi ADP1

43.807

83.586

0.366
  pilT Legionella pneumophila strain Lp02

45.171

81.061

0.366
  pilT Legionella pneumophila strain ERS1305867

45.171

81.061

0.366
  pilT Pseudomonas stutzeri DSM 10701

42.985

84.596

0.364