Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   HHM66_RS00785 Genome accession   NZ_CP053074
Coordinates   128044..128985 (+) Length   313 a.a.
NCBI ID   WP_170078124.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain TPCH-A88     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 123044..133985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHM66_RS00775 (HHM66_00775) rpoC 123755..127396 (+) 3642 WP_015057200.1 DNA-directed RNA polymerase subunit beta' -
  HHM66_RS00780 (HHM66_00780) - 127598..127963 (+) 366 WP_003055257.1 DUF1033 family protein -
  HHM66_RS00785 (HHM66_00785) comGA/cglA 128044..128985 (+) 942 WP_170078124.1 competence type IV pilus ATPase ComGA Machinery gene
  HHM66_RS00790 (HHM66_00790) comYB 128921..129952 (+) 1032 WP_015057201.1 competence type IV pilus assembly protein ComGB Machinery gene
  HHM66_RS00795 (HHM66_00795) comYC 129954..130280 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  HHM66_RS00800 (HHM66_00800) comGD 130255..130683 (+) 429 WP_041781473.1 competence type IV pilus minor pilin ComGD -
  HHM66_RS00805 (HHM66_00805) comGE 130691..130936 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  HHM66_RS00810 (HHM66_00810) comGF 130917..131357 (+) 441 WP_170078125.1 competence type IV pilus minor pilin ComGF -
  HHM66_RS00815 (HHM66_00815) comGG 131386..131709 (+) 324 WP_227985720.1 competence type IV pilus minor pilin ComGG -
  HHM66_RS00820 (HHM66_00820) comYH 131772..132725 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  HHM66_RS00825 (HHM66_00825) - 132784..133980 (+) 1197 WP_015057203.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35310.44 Da        Isoelectric Point: 7.0191

>NTDB_id=442751 HHM66_RS00785 WP_170078124.1 128044..128985(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain TPCH-A88]
MVQALARSILKEAEQIQAQDIYILPKEDQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IDGDTLLFLRLSSVGNYRGQESLVIRLLHHQQRSLYYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDDLLAAGHLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=442751 HHM66_RS00785 WP_170078124.1 128044..128985(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain TPCH-A88]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AGATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGATGGAGACACACTTTTGTTTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTATATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGCAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGCAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
ACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.701

99.361

0.623
  comYA Streptococcus mutans UA140

61.415

99.361

0.61
  comYA Streptococcus mutans UA159

61.415

99.361

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

61.093

99.361

0.607
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.361

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

52.09

99.361

0.518
  comGA Latilactobacillus sakei subsp. sakei 23K

39.116

93.93

0.367