Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HKW99_RS15515 Genome accession   NZ_CP053061
Coordinates   3304976..3306679 (-) Length   567 a.a.
NCBI ID   WP_407292131.1    Uniprot ID   -
Organism   Stutzerimonas zhaodongensis strain JZ60     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3299976..3311679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW99_RS15485 (HKW99_15415) - 3300040..3301077 (+) 1038 WP_407292116.1 NADP(H)-dependent aldo-keto reductase -
  HKW99_RS15490 (HKW99_15420) - 3301163..3301855 (+) 693 WP_407292118.1 energy-coupling factor ABC transporter permease -
  HKW99_RS15495 (HKW99_15425) yacG 3301892..3302089 (-) 198 WP_407292121.1 DNA gyrase inhibitor YacG -
  HKW99_RS15500 (HKW99_15430) coaE 3302086..3302694 (-) 609 WP_407292122.1 dephospho-CoA kinase -
  HKW99_RS15505 (HKW99_15435) pilD 3302888..3303754 (-) 867 WP_407292125.1 prepilin peptidase Machinery gene
  HKW99_RS15510 (HKW99_15440) pilC 3303757..3304974 (-) 1218 WP_407292128.1 type II secretion system F family protein Machinery gene
  HKW99_RS15515 (HKW99_15445) pilB 3304976..3306679 (-) 1704 WP_407292131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKW99_RS15520 (HKW99_15450) pilE 3306906..3307442 (+) 537 WP_407292133.1 pilin Machinery gene
  HKW99_RS15525 (HKW99_15455) tfpZ 3307573..3308304 (+) 732 WP_407292134.1 TfpX/TfpZ family type IV pilin accessory protein -
  HKW99_RS15530 (HKW99_15460) cysN 3308594..3310492 (-) 1899 WP_407292137.1 sulfate adenylyltransferase subunit CysN -
  HKW99_RS15535 (HKW99_15465) cysD 3310505..3311422 (-) 918 WP_407292138.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61874.79 Da        Isoelectric Point: 5.2252

>NTDB_id=442607 HKW99_RS15515 WP_407292131.1 3304976..3306679(-) (pilB) [Stutzerimonas zhaodongensis strain JZ60]
MTDNIALSGLAKQMVLTGLLDDKAAQQAQQQAARNKLSLVTYVVQNKLANGRAVAELAADQFGVAYMDLNALDKESQLKD
VVSEKLARQHRVLPLLKRGHKLFVAVSDPTNHQAVTDIQFSTGLTTEAILVEDDKLGLAIDKFFESATSGLEDLGEVDLD
GVDVQSVSDDGSSEDVGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAFRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALLEEGFP
ADKIGTFKIYGPVGCENCTGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=442607 HKW99_RS15515 WP_407292131.1 3304976..3306679(-) (pilB) [Stutzerimonas zhaodongensis strain JZ60]
ATGACGGACAACATTGCACTTTCCGGCCTCGCCAAACAGATGGTGCTAACCGGGCTGCTTGATGACAAGGCGGCGCAACA
AGCGCAACAGCAGGCTGCGCGTAATAAGCTGTCGCTGGTCACCTATGTCGTGCAGAACAAACTCGCCAATGGTCGTGCGG
TGGCTGAGTTGGCAGCCGACCAGTTCGGCGTTGCCTATATGGACCTTAACGCGCTGGATAAGGAGAGCCAGCTAAAGGAT
GTCGTCTCGGAAAAATTGGCCCGCCAGCACCGTGTGCTGCCATTGCTCAAGCGAGGCCATAAGCTGTTCGTGGCGGTTTC
TGATCCGACCAATCATCAGGCGGTGACGGATATCCAGTTCAGCACCGGGCTGACAACTGAAGCGATCCTCGTTGAAGATG
ACAAGCTCGGGTTGGCTATCGATAAATTCTTCGAGAGTGCTACATCCGGGCTGGAAGATCTAGGCGAGGTGGATCTGGAC
GGAGTGGACGTTCAGAGTGTGTCGGACGATGGGTCGAGCGAAGATGTGGGAGAAGCTGCTGATGATGCTCCCGTCGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGATCCTCCGACCTGCACTTCGAGCCTTATGAGAAAGCCTTTC
GAGTTCGCCTCCGTACGGATGGCATCCTGCACGAAGTCGCAAGGCCGCCGATCCAGCTAGCGCCCCGAATTTCTGCACGC
CTCAAGGTCATGGCTGGGCTGGACATATCCGAGCGGCGCAAACCGCAGGATGGTCGGATCAAGATGAAGCTGTCCAAGAC
CAAAGCCATCGACTTTCGCGTGAACACGTTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCTCCA
GTGCCCAGATGGGTATCGATGCGCTGGGTTATGAGGAAGACCAAAAGGAGTTGTACCTGAGCGCGCTCAAGCAGCCGCAG
GGCATGATCCTCGTTACCGGCCCAACGGGGTCGGGTAAGACAGTATCGCTCTATACCGGCCTGAACATTCTCAATACAGT
CGATGTAAACATCTCCACTGCGGAAGATCCGGTTGAGATCAACCTCGAAGGTATCAACCAAGTCAACGTTAACCCTCGTC
AAGGAATGGACTTCTCCCAGGCGCTTAGGGCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGGGAGATTCGCGAC
CTGGAGACTGCAGAGATCGCCATCAAGGCGGCACAGACGGGTCACATGGTGATGTCGACGCTTCATACGAACAGCGCCGC
AGAAACCCTGACACGCCTGCGCAACATGGGTGTGGCGGCCTTCAATATCGCCACGTCAGTGAACCTGATCATCGCCCAAC
GTCTGGCGCGAAAGCTGTGTAACAGTTGCAAGAAAGAAGTCGAATTGCCGCGTGAAGCACTGCTGGAAGAAGGATTTCCG
GCCGATAAGATTGGCACGTTCAAGATCTACGGTCCGGTGGGCTGCGAGAATTGCACAGGCGGCTATAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCAGCCCTGGCCAGGATTATCATGGAAGACGGCAATTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAATGACCTGCGGACTTCGGCTCTAGTCAAAGCCATGCAGGGCGTGACGAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.73

100

0.587
  pilB Acinetobacter baylyi ADP1

58.333

99.471

0.58
  pilB Legionella pneumophila strain ERS1305867

55.245

100

0.557
  pilB Vibrio parahaemolyticus RIMD 2210633

49.383

100

0.494
  pilB Vibrio cholerae strain A1552

54.043

89.418

0.483
  pilB Vibrio campbellii strain DS40M4

48.227

99.471

0.48
  pilF Neisseria gonorrhoeae MS11

52.115

91.711

0.478
  pilF Thermus thermophilus HB27

37.209

98.589

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.91

93.827

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

39.655

92.063

0.365