Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HKW99_RS15520 Genome accession   NZ_CP053061
Coordinates   3306906..3307442 (+) Length   178 a.a.
NCBI ID   WP_407292133.1    Uniprot ID   -
Organism   Stutzerimonas zhaodongensis strain JZ60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3301906..3312442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW99_RS15500 (HKW99_15430) coaE 3302086..3302694 (-) 609 WP_407292122.1 dephospho-CoA kinase -
  HKW99_RS15505 (HKW99_15435) pilD 3302888..3303754 (-) 867 WP_407292125.1 prepilin peptidase Machinery gene
  HKW99_RS15510 (HKW99_15440) pilC 3303757..3304974 (-) 1218 WP_407292128.1 type II secretion system F family protein Machinery gene
  HKW99_RS15515 (HKW99_15445) pilB 3304976..3306679 (-) 1704 WP_407292131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKW99_RS15520 (HKW99_15450) pilE 3306906..3307442 (+) 537 WP_407292133.1 pilin Machinery gene
  HKW99_RS15525 (HKW99_15455) tfpZ 3307573..3308304 (+) 732 WP_407292134.1 TfpX/TfpZ family type IV pilin accessory protein -
  HKW99_RS15530 (HKW99_15460) cysN 3308594..3310492 (-) 1899 WP_407292137.1 sulfate adenylyltransferase subunit CysN -
  HKW99_RS15535 (HKW99_15465) cysD 3310505..3311422 (-) 918 WP_407292138.1 sulfate adenylyltransferase subunit CysD -
  HKW99_RS15540 (HKW99_15470) - 3311597..3312355 (-) 759 WP_407292141.1 Nif3-like dinuclear metal center hexameric protein -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18213.59 Da        Isoelectric Point: 4.3466

>NTDB_id=442608 HKW99_RS15520 WP_407292133.1 3306906..3307442(+) (pilE) [Stutzerimonas zhaodongensis strain JZ60]
MKAQMQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLASSAKSLIAENANTQAELLAVATAWNAQANNSG
AVSKYVASVLVDDATGEVTVTFNEDNVGNIPAAATLVYTPYIQTGNGAVQLANNFGTGNTGSVDWGCASTANLVSAGRGL
TTLTAGTLPAQFAPSECR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=442608 HKW99_RS15520 WP_407292133.1 3306906..3307442(+) (pilE) [Stutzerimonas zhaodongensis strain JZ60]
ATGAAAGCTCAGATGCAAAAAGGTTTTACCCTGATTGAATTGATGATCGTTGTAGCGATCATTGGTATTTTGGCAGCAGT
GGCGTTGCCGGCTTATCAGGATTATACGATTCGAGCACGCGTTACCGAAGGTGTGGGTCTTGCCTCCAGTGCTAAGTCTC
TTATCGCAGAAAATGCAAATACCCAAGCCGAACTGCTTGCCGTAGCTACTGCATGGAATGCCCAAGCAAACAACTCTGGC
GCAGTAAGTAAATACGTCGCGTCCGTGCTAGTTGACGATGCTACTGGCGAAGTGACCGTTACGTTCAACGAAGACAACGT
TGGCAACATCCCAGCTGCTGCAACGCTGGTCTACACACCGTACATTCAAACTGGCAACGGCGCCGTTCAACTTGCCAACA
ACTTCGGCACCGGCAATACTGGTTCTGTTGATTGGGGCTGCGCTTCCACAGCCAACCTAGTTTCCGCCGGCCGCGGCCTT
ACAACTCTGACCGCTGGCACACTGCCTGCCCAGTTCGCTCCGAGCGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)

Pilin (bacterial filament) (Pilin)

(38-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

44.162

100

0.489
  pilA2 Legionella pneumophila str. Paris

44.509

97.191

0.433
  pilA2 Legionella pneumophila strain ERS1305867

43.353

97.191

0.421
  pilA Ralstonia pseudosolanacearum GMI1000

39.011

100

0.399