Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HC248_RS02630 Genome accession   NZ_CP051461
Coordinates   547406..548623 (+) Length   405 a.a.
NCBI ID   WP_168921143.1    Uniprot ID   -
Organism   Polaromonas vacuolata strain KCTC 22033     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542406..553623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC248_RS02610 (HC248_00522) rpmA 543549..543806 (-) 258 WP_168921140.1 50S ribosomal protein L27 -
  HC248_RS02615 (HC248_00523) rplU 543827..544147 (-) 321 WP_168921141.1 50S ribosomal protein L21 -
  HC248_RS02620 (HC248_00524) - 544342..545325 (+) 984 WP_202882408.1 polyprenyl synthetase family protein -
  HC248_RS02625 (HC248_00525) pilB 545587..547320 (+) 1734 WP_168921142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HC248_RS02630 (HC248_00526) pilC 547406..548623 (+) 1218 WP_168921143.1 type II secretion system F family protein Machinery gene
  HC248_RS02635 (HC248_00527) - 548660..549571 (+) 912 WP_202882409.1 GNAT family N-acetyltransferase -
  HC248_RS02640 (HC248_00528) - 549629..550519 (+) 891 WP_168921144.1 prepilin peptidase -
  HC248_RS02645 (HC248_00529) coaE 550523..551167 (+) 645 WP_168921145.1 dephospho-CoA kinase -
  HC248_RS02650 (HC248_00530) zapD 551207..551962 (+) 756 WP_168921146.1 cell division protein ZapD -
  HC248_RS02655 (HC248_00531) - 551982..552185 (+) 204 WP_168921147.1 DNA gyrase inhibitor YacG -
  HC248_RS02660 (HC248_00532) - 552290..552589 (+) 300 WP_238342700.1 hypothetical protein -
  HC248_RS02665 (HC248_00533) grxD 552667..552990 (-) 324 WP_168921148.1 Grx4 family monothiol glutaredoxin -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44125.92 Da        Isoelectric Point: 9.8325

>NTDB_id=438363 HC248_RS02630 WP_168921143.1 547406..548623(+) (pilC) [Polaromonas vacuolata strain KCTC 22033]
MATVASKGIQEFVYEWEGKDRAGKPVRGEVRAAGENQVKASLRRQGVLATKIKKRRMRSGKSIRPKDIAIFTRQLATMMK
AGVPLLQSFDIVGRGNPNASVTKLLNDIRSDVETGTSLSAAFRKYPLHFNSLYCNLVEAGEAAGILDSLLERLAVYMEKT
EAIKSKIKSALMYPISVVLVAFVVVAVIMIFVIPAFKSVFSSFGADLPAPTLMVIALSEFFVAYWWLIFGGLGGGFYFFM
QAWRRSPKMQRVMDRLVLKLPVFGSLINKSCIARWTRTLSTMFAAGVPLVEALDSVGGASGNSLYAYATEKIQQEVSTGT
SLTAAMSNANLFPTMVLQMCAIGEESGSVDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGTIIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=438363 HC248_RS02630 WP_168921143.1 547406..548623(+) (pilC) [Polaromonas vacuolata strain KCTC 22033]
ATGGCAACAGTTGCATCCAAGGGAATTCAAGAGTTTGTCTACGAATGGGAAGGCAAAGATCGTGCCGGTAAGCCTGTGCG
GGGAGAAGTTCGTGCTGCCGGTGAGAACCAAGTCAAGGCGTCTTTGCGACGTCAAGGCGTGTTGGCGACTAAGATTAAAA
AGCGCCGTATGCGCTCGGGGAAATCCATCCGGCCCAAGGATATTGCGATTTTCACGCGACAACTCGCCACCATGATGAAA
GCCGGCGTGCCCTTGCTGCAGTCGTTTGATATCGTTGGCCGCGGCAATCCAAATGCCAGTGTGACCAAGCTGCTCAACGA
TATTCGCTCGGATGTTGAAACCGGTACCTCGCTAAGCGCGGCCTTTCGAAAATACCCACTGCATTTCAATTCCTTGTATT
GCAATTTGGTCGAGGCCGGTGAGGCCGCTGGTATTTTGGATTCTTTGCTAGAGCGTCTAGCTGTTTACATGGAAAAAACT
GAGGCCATCAAGTCCAAGATCAAGTCCGCGCTGATGTATCCAATCTCTGTGGTGCTGGTGGCTTTTGTGGTTGTGGCGGT
AATCATGATTTTCGTCATACCAGCTTTTAAAAGTGTGTTTTCTTCGTTTGGCGCGGACCTGCCTGCGCCTACCTTGATGG
TTATCGCCTTGAGTGAATTTTTTGTTGCCTACTGGTGGTTGATTTTTGGCGGCTTGGGTGGTGGCTTTTATTTTTTCATG
CAGGCTTGGCGGCGTAGTCCCAAGATGCAAAGAGTCATGGATCGATTGGTGCTCAAGCTACCGGTGTTTGGCTCGCTGAT
TAATAAATCCTGCATCGCGCGCTGGACCCGTACGCTCTCGACTATGTTTGCCGCTGGCGTGCCTTTGGTCGAGGCGCTTG
ACTCGGTAGGCGGCGCTTCTGGTAATTCACTCTACGCTTACGCGACTGAAAAAATTCAGCAGGAAGTCTCTACTGGCACC
AGCTTAACGGCGGCCATGTCGAATGCCAATTTGTTTCCGACCATGGTGTTACAGATGTGCGCGATTGGGGAAGAATCCGG
ATCGGTTGACCACATGCTGGGTAAAGCCGCCGACTTTTACGAGGCCGAGGTCGATGAAATGGTGGCCGGCTTGTCCAGTT
TGATGGAACCCATCATCATCGTTTTTCTGGGCACAATAATTGGCGGCATTGTGGTTTCCATGTACTTGCCCATTTTTAAG
CTCGGACAGGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.086

100

0.531
  pilC Legionella pneumophila strain ERS1305867

51.759

98.272

0.509
  pilG Neisseria gonorrhoeae MS11

51.134

98.025

0.501
  pilG Neisseria meningitidis 44/76-A

50.882

98.025

0.499
  pilC Acinetobacter baylyi ADP1

47.99

98.272

0.472
  pilC Acinetobacter baumannii D1279779

47.859

98.025

0.469
  pilC Vibrio cholerae strain A1552

42.462

98.272

0.417
  pilC Vibrio campbellii strain DS40M4

40.5

98.765

0.4
  pilC Thermus thermophilus HB27

38.462

99.506

0.383