Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HC248_RS02625 Genome accession   NZ_CP051461
Coordinates   545587..547320 (+) Length   577 a.a.
NCBI ID   WP_168921142.1    Uniprot ID   A0A6H2H656
Organism   Polaromonas vacuolata strain KCTC 22033     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540587..552320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC248_RS02600 (HC248_00520) proB 541123..542259 (-) 1137 WP_168921138.1 glutamate 5-kinase -
  HC248_RS02605 (HC248_00521) cgtA 542351..543424 (-) 1074 WP_168921139.1 Obg family GTPase CgtA -
  HC248_RS02610 (HC248_00522) rpmA 543549..543806 (-) 258 WP_168921140.1 50S ribosomal protein L27 -
  HC248_RS02615 (HC248_00523) rplU 543827..544147 (-) 321 WP_168921141.1 50S ribosomal protein L21 -
  HC248_RS02620 (HC248_00524) - 544342..545325 (+) 984 WP_202882408.1 polyprenyl synthetase family protein -
  HC248_RS02625 (HC248_00525) pilB 545587..547320 (+) 1734 WP_168921142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HC248_RS02630 (HC248_00526) pilC 547406..548623 (+) 1218 WP_168921143.1 type II secretion system F family protein Machinery gene
  HC248_RS02635 (HC248_00527) - 548660..549571 (+) 912 WP_202882409.1 GNAT family N-acetyltransferase -
  HC248_RS02640 (HC248_00528) - 549629..550519 (+) 891 WP_168921144.1 prepilin peptidase -
  HC248_RS02645 (HC248_00529) coaE 550523..551167 (+) 645 WP_168921145.1 dephospho-CoA kinase -
  HC248_RS02650 (HC248_00530) zapD 551207..551962 (+) 756 WP_168921146.1 cell division protein ZapD -
  HC248_RS02655 (HC248_00531) - 551982..552185 (+) 204 WP_168921147.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62958.61 Da        Isoelectric Point: 5.3222

>NTDB_id=438362 HC248_RS02625 WP_168921142.1 545587..547320(+) (pilB) [Polaromonas vacuolata strain KCTC 22033]
MATADSAINEISSMALPGLGRALVVAGKIGQRAAEDIFAKAKNSRTSFIAELTGSGVVSPFDLAHIMSQAFAAPLLDLDA
VDIMRLPRDLLDAKICQTYRIVVLGKRNNRLIVATADPSDQDAAEKIKFSTQLGVDWIIAEYDKLSQMLAASSATATQAM
DKIIGDDFEFDESTIESITDTTDPSVSEVDDAPVVKFLQKMLLDAFSMRASDLHFEPFEHSYRVRFRVDGELREIASPPV
AIKEKLAARIKVISRLDISEKRVPQDGKMKLKIGPDRVIDFRVSSLPTMFGEKIVIRILDPSSAKLGIDALGYEPIEKAR
LLQAIGRPYGMVLVTGPTSSGKTVSLYTCLNILNKPGVNISTAEDPAEITMPGVNQVSMNEKAGMTFPVALKAFLRQDPD
IIMVGEIRDIETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTNCKMPTDIPR
QTLLEAGFQEDDVDGSWTPYRPVGCSTCNNGYKGRFGIYQVMPITEEIQRIILRDGSALEIAAQAESEGVRSLRQSGLYK
VKLGMTSLEEILGCTNV

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=438362 HC248_RS02625 WP_168921142.1 545587..547320(+) (pilB) [Polaromonas vacuolata strain KCTC 22033]
ATGGCCACTGCAGACTCTGCGATCAATGAAATTTCTTCCATGGCTTTGCCTGGTTTGGGCCGCGCACTGGTGGTGGCCGG
GAAGATTGGTCAGCGCGCGGCGGAAGATATTTTTGCCAAAGCTAAAAACAGTCGCACCAGTTTTATCGCCGAGCTGACCG
GATCAGGTGTTGTTTCCCCTTTCGATTTGGCGCACATCATGTCGCAGGCTTTTGCCGCGCCTTTGCTCGACCTTGATGCG
GTAGACATCATGCGTTTGCCGCGGGATTTGCTGGATGCCAAAATCTGCCAGACTTACCGCATCGTTGTGCTGGGTAAGCG
TAACAACCGCTTAATTGTGGCGACGGCCGATCCCTCCGACCAAGATGCCGCTGAAAAAATCAAATTTTCTACGCAGCTAG
GCGTGGACTGGATTATTGCGGAGTACGACAAGCTCTCGCAAATGCTGGCGGCGTCTAGTGCTACCGCCACACAAGCAATG
GACAAAATCATTGGTGATGATTTTGAATTTGATGAATCCACCATTGAAAGCATCACAGACACAACTGACCCTAGTGTGTC
CGAAGTCGATGACGCTCCAGTGGTTAAGTTTTTGCAAAAAATGCTGCTTGATGCATTCAGTATGCGAGCTTCTGATTTGC
ATTTCGAACCGTTTGAACACAGCTATCGGGTCCGGTTTAGGGTTGATGGCGAACTGCGTGAAATTGCCTCGCCGCCGGTG
GCGATTAAAGAAAAGCTGGCCGCTCGCATCAAGGTAATTTCTCGGCTAGATATCTCTGAAAAACGCGTGCCGCAAGACGG
CAAGATGAAGTTGAAAATTGGGCCAGACCGGGTGATCGATTTTCGCGTCAGCTCCTTGCCCACTATGTTTGGCGAGAAAA
TCGTGATTCGTATTCTCGACCCTAGTAGCGCCAAACTAGGCATTGATGCGTTGGGCTACGAGCCGATTGAAAAAGCCCGT
CTGCTGCAAGCGATCGGCCGACCCTACGGCATGGTGTTGGTCACCGGGCCGACGAGCTCGGGCAAAACGGTTTCGCTTTA
TACCTGCCTGAATATTTTGAATAAGCCGGGCGTGAATATTTCCACCGCAGAAGATCCGGCCGAGATCACCATGCCTGGCG
TGAACCAAGTCAGCATGAATGAAAAAGCAGGCATGACATTTCCGGTTGCGCTCAAGGCATTTTTGCGCCAAGACCCAGAC
ATCATCATGGTGGGCGAGATTCGCGACATCGAAACGGCAGACATTTCCATCAAGGCCGCGCAGACCGGTCACTTGGTGTT
GTCAACTCTGCACACCAACGATGCGCCCACTACTTTGACGCGTATGCGCAATATGGGCATAGCGCCTTTTAATATCGCCT
CTAGTGTGATTCTCATTACTGCTCAGAGACTGGCGCGTCGACTCTGCACCAACTGCAAGATGCCAACAGATATTCCGCGT
CAAACCTTGCTCGAAGCGGGCTTTCAGGAAGACGATGTCGATGGCAGCTGGACACCTTACCGGCCGGTAGGTTGCTCTAC
GTGCAACAACGGCTACAAAGGGCGGTTTGGCATTTATCAGGTGATGCCTATCACTGAAGAAATTCAACGCATTATTTTGC
GCGACGGCAGTGCACTTGAAATCGCCGCGCAGGCTGAATCTGAAGGTGTCAGAAGCTTGCGTCAGTCGGGCTTATACAAA
GTCAAGCTTGGTATGACTTCGCTAGAAGAAATCTTAGGTTGTACCAATGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H2H656

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.996

97.574

0.527
  pilB Acinetobacter baumannii D1279779

52.817

98.44

0.52
  pilF Neisseria gonorrhoeae MS11

49.466

97.4

0.482
  pilB Legionella pneumophila strain ERS1305867

49.286

97.054

0.478
  pilB Vibrio campbellii strain DS40M4

43.262

97.747

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

44

95.321

0.419
  pilB Vibrio cholerae strain A1552

45.224

88.908

0.402
  pilF Thermus thermophilus HB27

36.861

98.267

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.098

92.201

0.36