Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   FOB43_RS11315 Genome accession   NZ_CP050992
Coordinates   2380050..2380517 (+) Length   155 a.a.
NCBI ID   WP_168227762.1    Uniprot ID   -
Organism   Chromobacterium violaceum strain FDAARGOS_635     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2375050..2385517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB43_RS11300 (FOB43_11300) proB 2375650..2376768 (-) 1119 WP_168227759.1 glutamate 5-kinase -
  FOB43_RS11305 (FOB43_11305) - 2376844..2378346 (-) 1503 WP_168227760.1 hypothetical protein -
  FOB43_RS11310 (FOB43_11310) - 2378429..2379775 (-) 1347 WP_168227761.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  FOB43_RS11315 (FOB43_11315) pilA2 2380050..2380517 (+) 468 WP_168227762.1 pilin Machinery gene
  FOB43_RS11320 (FOB43_11320) - 2380599..2381075 (+) 477 WP_168227763.1 pilin -
  FOB43_RS11325 (FOB43_11325) - 2381144..2383006 (+) 1863 WP_168227764.1 Wzy polymerase domain-containing protein -
  FOB43_RS11330 (FOB43_11330) - 2383089..2384873 (+) 1785 WP_168227765.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16091.25 Da        Isoelectric Point: 8.9315

>NTDB_id=435798 FOB43_RS11315 WP_168227762.1 2380050..2380517(+) (pilA2) [Chromobacterium violaceum strain FDAARGOS_635]
MKKQRKQQGFTLIELMIVVAIVGILAAIAIPAYQDYTKRARVSEGLALADAAKTAVTEYYSTNNAFASGTTPLNTAYGVA
ASVTGNSVSGVSVLANGVIQITYNGTVSNGALVDLVPTVTSGSVQWYCTYTGSGTTAIATANQLSANWVPSTCRQ

Nucleotide


Download         Length: 468 bp        

>NTDB_id=435798 FOB43_RS11315 WP_168227762.1 2380050..2380517(+) (pilA2) [Chromobacterium violaceum strain FDAARGOS_635]
ATGAAGAAACAGCGTAAACAGCAGGGCTTCACCCTGATCGAACTGATGATCGTGGTCGCCATCGTCGGCATCCTGGCCGC
CATCGCCATTCCGGCCTATCAGGACTACACCAAGCGCGCGCGGGTGTCGGAAGGGTTGGCGCTGGCCGACGCGGCCAAGA
CGGCGGTGACGGAGTATTATTCGACGAATAACGCGTTCGCTTCCGGTACGACGCCGCTCAATACCGCGTATGGGGTGGCG
GCGTCGGTTACCGGCAATTCGGTTAGTGGCGTCAGTGTATTGGCGAATGGTGTCATCCAAATTACTTATAACGGCACGGT
ATCGAATGGCGCGCTGGTGGACTTGGTGCCGACCGTTACGTCGGGTTCGGTTCAGTGGTATTGCACTTACACCGGGTCGG
GTACGACGGCAATTGCGACGGCCAATCAGTTGAGCGCCAATTGGGTGCCGTCAACTTGCCGCCAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(39-154)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.667

96.774

0.51
  pilA2 Legionella pneumophila strain ERS1305867

52

96.774

0.503
  pilE Neisseria gonorrhoeae strain FA1090

45.625

100

0.471
  pilE Neisseria gonorrhoeae MS11

44.72

100

0.465
  pilA Ralstonia pseudosolanacearum GMI1000

42.169

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.234

100

0.452
  comP Acinetobacter baylyi ADP1

44.516

100

0.445
  pilA/pilA1 Eikenella corrodens VA1

35.22

100

0.361
  pilA/pilAI Pseudomonas stutzeri DSM 10701

35.22

100

0.361