Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HCU79_RS18815 Genome accession   NZ_CP050794
Coordinates   4125417..4126658 (+) Length   413 a.a.
NCBI ID   WP_202047337.1    Uniprot ID   A0AAW5RK11
Organism   Aeromonas media strain FiBL-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4120417..4131658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU79_RS18790 (HCU79_18780) ampD 4120756..4121328 (-) 573 WP_404837243.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HCU79_RS18795 (HCU79_18785) - 4121466..4121936 (+) 471 WP_139439015.1 TIGR02281 family clan AA aspartic protease -
  HCU79_RS18800 (HCU79_18790) nadC 4121940..4122803 (+) 864 WP_404837244.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HCU79_RS18805 - 4123116..4123577 (+) 462 WP_404837245.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HCU79_RS18810 (HCU79_18800) pilB 4123581..4125287 (+) 1707 WP_404837246.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HCU79_RS18815 (HCU79_18805) pilC 4125417..4126658 (+) 1242 WP_202047337.1 type II secretion system F family protein Machinery gene
  HCU79_RS18820 (HCU79_18810) pilD 4126739..4127611 (+) 873 WP_404837247.1 prepilin peptidase Machinery gene
  HCU79_RS18825 (HCU79_18815) coaE 4127630..4128244 (+) 615 WP_404837248.1 dephospho-CoA kinase -
  HCU79_RS18830 (HCU79_18820) zapD 4128282..4129004 (+) 723 WP_162519286.1 cell division protein ZapD -
  HCU79_RS18835 (HCU79_18825) yacG 4129017..4129211 (+) 195 WP_042053535.1 DNA gyrase inhibitor YacG -
  HCU79_RS18840 (HCU79_18830) mutT 4129283..4129687 (-) 405 WP_247625411.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45577.64 Da        Isoelectric Point: 10.0689

>NTDB_id=434906 HCU79_RS18815 WP_202047337.1 4125417..4126658(+) (pilC) [Aeromonas media strain FiBL-01]
MATLAQKRNTPKKVFSFRWHGFNRKGQKVSGEFQADNINTVKAELRKQGVNVTKVSKQGQGLFSKGGARIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIATDVETGTPLSEALRRHPRHFDALYCDLVDAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIVVTSILLLFVIPQFEEIFKSFGAELPVFTRFVIGISRFMQDWWYIIFGGIA
LAIFLYVRAWRKSQKVRDSTDRFILTIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAILAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=434906 HCU79_RS18815 WP_202047337.1 4125417..4126658(+) (pilC) [Aeromonas media strain FiBL-01]
ATGGCAACATTAGCGCAAAAACGTAACACACCCAAAAAGGTCTTCTCCTTCCGCTGGCATGGATTCAACCGCAAGGGGCA
GAAGGTTTCCGGTGAGTTCCAGGCCGACAACATCAACACCGTCAAGGCGGAGCTGCGCAAGCAGGGCGTCAATGTCACCA
AGGTCAGCAAGCAGGGCCAGGGACTGTTTTCCAAAGGTGGTGCCCGGATCAAGCCGATGGACATCGCCGTCATCTCACGC
CAGATCACCACCATGCTCTCCGCCGGTGTGCCACTGGTGCAGAGCCTGCAGATCATCGCCCGCGGTCATGAGAAGGCGGC
GGTACGCGAACTCATCGGTCAGATCGCCACCGATGTGGAAACCGGCACCCCACTGTCGGAAGCGCTGCGCCGCCACCCAC
GCCACTTCGATGCCCTCTACTGCGATCTGGTGGACGCTGGGGAGCAGTCCGGTGCGCTGGAAACCATCTACGACCGAATT
GCCACATACCGTGAGAAGAGTGAGGCGCTCAAGTCCAAGATCAAGAAGGCCATGTTCTATCCCGCCATGGTCATCCTGGT
CGCCATCGTCGTGACCTCCATACTGCTGCTGTTCGTCATTCCCCAGTTCGAGGAGATCTTCAAGAGCTTCGGGGCCGAGC
TGCCAGTCTTCACCCGATTCGTCATTGGCATCTCCCGCTTCATGCAGGATTGGTGGTATATCATCTTTGGCGGCATAGCC
CTCGCCATCTTCCTCTACGTGCGCGCCTGGCGAAAATCCCAGAAGGTACGGGACAGCACCGACAGATTCATCCTCACTAT
TCCGGTCGTGGGCAACATACTGCACAAGGCGGCCATGGCCCGCTTCGCCCGCACCCTCTCCACCACCTTCTCCGCCGGGA
TTCCCTTGGTGGATGCCCTGGTCTCCGCTGCCGGCGCCTCTGGCAACTATGTCTATCGCACGGCCATCCTGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTCGCCATGCGCACCGTGGATCTCTTCCCCGACATGGTGATCCAGATGGT
GATGATAGGTGAAGAGTCCGGCGCCATCGATGACATGCTCTCCAAGGTTGCCGCCATCTTCGAGCAGGAGGTGGACGACA
TGGTCGATGGCCTCACCAGCCTGCTCGAGCCCATCATCATGGTGGTGCTCGGGGTGCTGGTCGGTGGCATGGTCGTCGCC
ATGTACCTCCCCATCTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.061

95.884

0.538
  pilC Legionella pneumophila strain ERS1305867

52.593

98.063

0.516
  pilC Acinetobacter baumannii D1279779

51.87

97.094

0.504
  pilC Acinetobacter baylyi ADP1

51.637

96.126

0.496
  pilC Vibrio cholerae strain A1552

47.5

96.852

0.46
  pilC Vibrio campbellii strain DS40M4

46.482

96.368

0.448
  pilG Neisseria gonorrhoeae MS11

40.05

97.337

0.39
  pilG Neisseria meningitidis 44/76-A

39.801

97.337

0.387
  pilC Thermus thermophilus HB27

38.75

96.852

0.375