Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HB764_RS12740 Genome accession   NZ_CP050478
Coordinates   2728499..2730184 (+) Length   561 a.a.
NCBI ID   WP_255957061.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2723499..2735184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB764_RS12720 (HB764_12605) pdhR 2725089..2725856 (-) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HB764_RS12725 (HB764_12610) ampD 2726261..2726812 (-) 552 WP_134976391.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HB764_RS12730 (HB764_12615) nadC 2726905..2727792 (+) 888 WP_255957059.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HB764_RS12735 (HB764_12620) pilA 2728056..2728487 (+) 432 WP_255957060.1 pilin Machinery gene
  HB764_RS12740 (HB764_12625) pilB 2728499..2730184 (+) 1686 WP_255957061.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HB764_RS12745 (HB764_12630) pilC 2730219..2731442 (+) 1224 WP_255957062.1 type II secretion system F family protein Machinery gene
  HB764_RS12750 (HB764_12635) pilD 2731512..2732381 (+) 870 WP_255957063.1 A24 family peptidase Machinery gene
  HB764_RS12755 (HB764_12640) coaE 2732382..2732996 (+) 615 WP_255957064.1 dephospho-CoA kinase -
  HB764_RS12760 (HB764_12645) zapD 2733024..2733764 (+) 741 WP_005534051.1 cell division protein ZapD -
  HB764_RS12765 (HB764_12650) yacG 2733821..2734021 (+) 201 WP_012128882.1 DNA gyrase inhibitor YacG -
  HB764_RS12770 (HB764_12655) rplS 2734359..2734712 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62076.86 Da        Isoelectric Point: 5.1851

>NTDB_id=433208 HB764_RS12740 WP_255957061.1 2728499..2730184(+) (pilB) [Vibrio campbellii strain LJC011]
MLTNLSTVLRQAGILTFSQEESLLEQVKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEGEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLNLIIAQRLARKLCPYCRQPQEPNNQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=433208 HB764_RS12740 WP_255957061.1 2728499..2730184(+) (pilB) [Vibrio campbellii strain LJC011]
ATGCTAACCAACCTCTCAACGGTTCTTCGCCAAGCAGGAATACTGACCTTTTCCCAAGAAGAATCGTTACTTGAACAAGT
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTAACAGAACACCTAA
GCTCAGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATACGCTTCGCTATGCCAAACGCTCGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAACTTTGATTCTCGCGGTTGCCGACCCAACAAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCGACAGGCTTGCAAGTCGAGCTTGTCCTCGCTGACTTTCGTGAGCTAACAGCAG
CTATTCGACGTTTATATGGCCGATCACTTGGGCAAGAAAAATCCGGCCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGGTGAAATTGAGAACATCGAAGATCTCAGCCAAGATGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTCTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCCTATGAAAACATGTACCGCGTTCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCACCGAGTCATTTAAGCCGTCGTCTCTCCGCTCGGATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTGCCACAGGATGGGCGCATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTGTTTGGAGAAAAAATTGTTCTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAGCTCGGTTATAGCGACTCACAAAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAGCATTCTTAATAAACCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTTGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCTAAGATCGGTTTTG
GTTTTGCCGAAGCCTTGCGCTCTTTCTTACGTCAAGATCCTGATGTAGTCATGGTAGGTGAGATTCGAGACCTCGATACG
GCAGAAATCGCTATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTGCACACAAACTCAGCTGCCGAGACTGT
TATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCCTCTCTCAATTTGATCATCGCCCAACGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAACCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTTTCAGAAGCACTCATCAAAGGCGCCTCGGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCTTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.752

100

0.988
  pilB Vibrio parahaemolyticus RIMD 2210633

90.374

100

0.904
  pilB Vibrio cholerae strain A1552

73.31

100

0.734
  pilB Acinetobacter baumannii D1279779

51.445

92.513

0.476
  pilB Legionella pneumophila strain ERS1305867

47.576

99.287

0.472
  pilB Acinetobacter baylyi ADP1

50.484

92.157

0.465
  pilF Neisseria gonorrhoeae MS11

43.262

100

0.435
  pilB/pilB1 Synechocystis sp. PCC 6803

36.762

100

0.389
  pilF Thermus thermophilus HB27

36.833

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.559

99.465

0.364