Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HB764_RS12735 Genome accession   NZ_CP050478
Coordinates   2728056..2728487 (+) Length   143 a.a.
NCBI ID   WP_255957060.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC011     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2723056..2733487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB764_RS12720 (HB764_12605) pdhR 2725089..2725856 (-) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HB764_RS12725 (HB764_12610) ampD 2726261..2726812 (-) 552 WP_134976391.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HB764_RS12730 (HB764_12615) nadC 2726905..2727792 (+) 888 WP_255957059.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HB764_RS12735 (HB764_12620) pilA 2728056..2728487 (+) 432 WP_255957060.1 pilin Machinery gene
  HB764_RS12740 (HB764_12625) pilB 2728499..2730184 (+) 1686 WP_255957061.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HB764_RS12745 (HB764_12630) pilC 2730219..2731442 (+) 1224 WP_255957062.1 type II secretion system F family protein Machinery gene
  HB764_RS12750 (HB764_12635) pilD 2731512..2732381 (+) 870 WP_255957063.1 A24 family peptidase Machinery gene
  HB764_RS12755 (HB764_12640) coaE 2732382..2732996 (+) 615 WP_255957064.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14574.54 Da        Isoelectric Point: 6.2300

>NTDB_id=433207 HB764_RS12735 WP_255957060.1 2728056..2728487(+) (pilA) [Vibrio campbellii strain LJC011]
MKTNKQKKQQGFTLIELMIVVAIIGVLAAVAIPAYQDYVKKSEAASALATLKSLITPAELYYQENGTSTAAGLSDLGSVA
SANDLGTLTSAIGSGSGGVPTLKFAFGSNSSMASTDTLTFSRSATEGWSCARAGTVPAIDGCQ

Nucleotide


Download         Length: 432 bp        

>NTDB_id=433207 HB764_RS12735 WP_255957060.1 2728056..2728487(+) (pilA) [Vibrio campbellii strain LJC011]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTGATGATTGTGGTAGCGATTATTGGTGTTTT
GGCAGCGGTTGCTATACCAGCTTATCAAGACTACGTTAAAAAATCAGAAGCCGCATCAGCTCTTGCTACCTTAAAATCCT
TGATTACTCCGGCTGAACTCTATTATCAAGAAAATGGAACGAGCACAGCTGCTGGTCTTTCTGATTTAGGTTCAGTAGCT
TCAGCTAACGATTTAGGCACACTAACATCGGCAATTGGATCTGGTTCAGGTGGAGTACCAACTCTAAAGTTTGCTTTTGG
TTCAAACAGCTCTATGGCCTCGACTGATACTTTAACTTTCTCTCGTAGTGCAACCGAAGGTTGGTCTTGTGCTCGTGCTG
GTACAGTCCCAGCAATTGATGGCTGCCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

61.972

99.301

0.615
  pilA Pseudomonas aeruginosa PAK

44.444

94.406

0.42
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.066

95.804

0.413
  pilA Vibrio cholerae strain A1552

43.066

95.804

0.413
  pilA Vibrio cholerae C6706

43.066

95.804

0.413
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.481

94.406

0.392