Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HB763_RS01975 Genome accession   NZ_CP050475
Coordinates   447090..448775 (+) Length   561 a.a.
NCBI ID   WP_255985277.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC012     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442090..453775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB763_RS01955 (HB763_01960) pdhR 443695..444462 (-) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HB763_RS01960 (HB763_01965) ampD 444867..445418 (-) 552 WP_255985276.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HB763_RS01965 (HB763_01970) nadC 445511..446398 (+) 888 WP_010643264.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HB763_RS01970 (HB763_01975) pilA 446662..447090 (+) 429 WP_050912911.1 type IV pilin protein Machinery gene
  HB763_RS01975 (HB763_01980) pilB 447090..448775 (+) 1686 WP_255985277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HB763_RS01980 (HB763_01985) pilC 448810..450033 (+) 1224 WP_255985278.1 type II secretion system F family protein Machinery gene
  HB763_RS01985 (HB763_01990) pilD 450106..450975 (+) 870 WP_255985279.1 A24 family peptidase Machinery gene
  HB763_RS01990 (HB763_01995) coaE 450976..451590 (+) 615 WP_255985280.1 dephospho-CoA kinase -
  HB763_RS01995 (HB763_02000) zapD 451618..452358 (+) 741 WP_005534051.1 cell division protein ZapD -
  HB763_RS02000 (HB763_02005) yacG 452415..452615 (+) 201 WP_012128882.1 DNA gyrase inhibitor YacG -
  HB763_RS02005 (HB763_02010) rplS 452954..453307 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62138.88 Da        Isoelectric Point: 5.0310

>NTDB_id=433138 HB763_RS01975 WP_255985277.1 447090..448775(+) (pilB) [Vibrio campbellii strain LJC012]
MLTNLSTVLRQAGILTFSQEESLLEQVKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANDMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=433138 HB763_RS01975 WP_255985277.1 447090..448775(+) (pilB) [Vibrio campbellii strain LJC012]
ATGCTAACCAACCTTTCAACGGTTCTTCGTCAAGCAGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGT
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTAACGGAGCACCTAA
GCTCAGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATACGCTTCGCTATGCCAAACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAACTTTAATTCTCGCGGTAGCTGACCCAACCAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCAACCGGCTTGCAAGTCGAGCTTGTCCTCGCTGACTTTCGCGAGCTGACAGCAG
CCATTCGGCGTTTGTATGGCCGATCGCTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGACGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAACCTTACGAAAATATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGAGTCATTTAAGCCGTCGCCTTTCAGCTCGGATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTACCACAGGATGGGCGAATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTGTTTGGAGAAAAGATTGTTCTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAGCTCGGTTATAGCGACTCACAAAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAGTATTCTTAATAAGCCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTCGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAAGCGCTCCGCTCTTTCTTACGTCAAGATCCTGATGTCGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTACACACAAACTCAGCTGCCGAGACTGT
TATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCTTCTCTCAGTTTGATCATCGCCCAACGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAATGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGACGAATCCCTTTCAGAAGCACTCATCAAAGGCGCTTCAGTACACGAATTAGAAAAACTCGCCATTGCCAATGATA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

99.109

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

90.553

100

0.906
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Acinetobacter baumannii D1279779

51.434

93.226

0.48
  pilB Legionella pneumophila strain ERS1305867

47.576

99.287

0.472
  pilB Acinetobacter baylyi ADP1

50.097

92.157

0.462
  pilF Neisseria gonorrhoeae MS11

43.44

100

0.437
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilF Thermus thermophilus HB27

36.655

100

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.38

99.465

0.362