Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HB763_RS01970 Genome accession   NZ_CP050475
Coordinates   446662..447090 (+) Length   142 a.a.
NCBI ID   WP_050912911.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC012     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441662..452090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB763_RS01955 (HB763_01960) pdhR 443695..444462 (-) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HB763_RS01960 (HB763_01965) ampD 444867..445418 (-) 552 WP_255985276.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HB763_RS01965 (HB763_01970) nadC 445511..446398 (+) 888 WP_010643264.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HB763_RS01970 (HB763_01975) pilA 446662..447090 (+) 429 WP_050912911.1 type IV pilin protein Machinery gene
  HB763_RS01975 (HB763_01980) pilB 447090..448775 (+) 1686 WP_255985277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HB763_RS01980 (HB763_01985) pilC 448810..450033 (+) 1224 WP_255985278.1 type II secretion system F family protein Machinery gene
  HB763_RS01985 (HB763_01990) pilD 450106..450975 (+) 870 WP_255985279.1 A24 family peptidase Machinery gene
  HB763_RS01990 (HB763_01995) coaE 450976..451590 (+) 615 WP_255985280.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14510.54 Da        Isoelectric Point: 5.7554

>NTDB_id=433137 HB763_RS01970 WP_050912911.1 446662..447090(+) (pilA) [Vibrio campbellii strain LJC012]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSEAAAGLATLRSLTTNIDTFIADTGTFPADGDAPTLGAA
VDMNKLGTISFADSGASGATATFTFDGTASALASTDTVVLAKDATTGLWTCSHSTGVTLKGC

Nucleotide


Download         Length: 429 bp        

>NTDB_id=433137 HB763_RS01970 WP_050912911.1 446662..447090(+) (pilA) [Vibrio campbellii strain LJC012]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACCCTAATTGAATTGATGATCGTGGTAGCTGTTATTGGTGTTTT
AGCAGCGATTGCTATTCCACAGTATCAGAATTATGTAAAAAAATCAGAGGCTGCAGCTGGGCTTGCAACTCTTCGTTCAC
TAACTACAAATATTGATACTTTCATTGCTGATACTGGCACCTTTCCTGCTGATGGCGACGCACCCACATTAGGTGCTGCT
GTTGATATGAACAAACTTGGAACAATTTCTTTTGCAGATAGTGGTGCATCGGGAGCGACAGCAACGTTTACTTTTGATGG
TACAGCTAGCGCACTAGCTTCTACTGATACCGTTGTCCTTGCGAAGGATGCAACTACCGGACTTTGGACCTGCTCTCATA
GTACTGGCGTAACTCTAAAAGGCTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.299

100

0.556
  pilA Vibrio cholerae strain A1552

51.299

100

0.556
  pilA Vibrio cholerae C6706

51.299

100

0.556
  pilA Vibrio parahaemolyticus RIMD 2210633

48.252

100

0.486
  pilA Pseudomonas aeruginosa PAK

45.27

100

0.472
  pilA Acinetobacter baumannii strain A118

35.948

100

0.387