Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HB760_RS15020 Genome accession   NZ_CP050464
Coordinates   32309..34063 (-) Length   584 a.a.
NCBI ID   WP_255901950.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC015     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 27309..39063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB760_RS15000 (HB760_14925) - 28014..28853 (-) 840 WP_038889578.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  HB760_RS15005 (HB760_14930) - 28923..30416 (-) 1494 WP_255899055.1 SPOR domain-containing protein -
  HB760_RS15010 (HB760_14935) aroB 30470..31570 (-) 1101 WP_255899052.1 3-dehydroquinate synthase -
  HB760_RS15015 (HB760_14940) aroK 31602..32120 (-) 519 WP_010443904.1 shikimate kinase AroK -
  HB760_RS15020 (HB760_14945) pilQ 32309..34063 (-) 1755 WP_255901950.1 type IV pilus secretin PilQ Machinery gene
  HB760_RS15025 (HB760_14950) pilP 34098..34613 (-) 516 WP_255899049.1 pilus assembly protein PilP Machinery gene
  HB760_RS15030 (HB760_14955) pilO 34603..35196 (-) 594 WP_255899047.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB760_RS15035 (HB760_14960) pilN 35189..35764 (-) 576 WP_256113731.1 PilN domain-containing protein Machinery gene
  HB760_RS15040 (HB760_14965) pilM 35748..36764 (-) 1017 WP_255899045.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63942.80 Da        Isoelectric Point: 4.4499

>NTDB_id=433028 HB760_RS15020 WP_255901950.1 32309..34063(-) (pilQ) [Vibrio campbellii strain LJC015]
MKMRHGLTLTPRKLTGLFSLWFSLLLAPFVAAEEVQSNSLESIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGLPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGEGEGEGDDGGSSVGIDDFLNVNLAATSP
NATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVT
PQITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGAL
FRRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=433028 HB760_RS15020 WP_255901950.1 32309..34063(-) (pilQ) [Vibrio campbellii strain LJC015]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTCTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTGAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTCGAGGGTAACTTGACCTTGCGTTTGGATGGTTTGCCTTGGCAGCAAGTACTCGACATTATCCTGCAAGTAA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTCGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGAGAGGGCAACATCAGTATGCTTTCAGATCGAGGCAGCATGACCATTGATGAGCGTACCA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACCTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCTTGTACGATGGCGGCGGTGAAG
GTGAAGGTGAAGGTGATGACGGTGGCAGCAGTGTAGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCA
AATGCGACCAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACA
AGAATCGAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCG
AGATCCCTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACG
CCACAGATTACGCCTGACAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCAGGCCAAGTGGTAAAAACAGG
CACTGGTGAGGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTCAATAATGGCGAGACAGTCGTATTGG
GCGGTATTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTT
TTCCGTCGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(424-582)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

98.63

100

0.986
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.193

97.603

0.753
  pilQ Vibrio cholerae strain A1552

77.193

97.603

0.753
  pilQ Legionella pneumophila strain ERS1305867

40.82

96.062

0.392
  pilQ Legionella pneumophila strain Lp02

41.198

94.349

0.389
  pilQ Pseudomonas aeruginosa PAK

39.859

97.089

0.387
  pilQ Acinetobacter baumannii strain A118

37.105

100

0.382
  pilQ Acinetobacter baumannii D1279779

37.105

100

0.382
  comQ Acinetobacter baylyi ADP1

36.707

99.829

0.366