Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HB760_RS15040 Genome accession   NZ_CP050464
Coordinates   35748..36764 (-) Length   338 a.a.
NCBI ID   WP_255899045.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC015     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 30748..41764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB760_RS15015 (HB760_14940) aroK 31602..32120 (-) 519 WP_010443904.1 shikimate kinase AroK -
  HB760_RS15020 (HB760_14945) pilQ 32309..34063 (-) 1755 WP_255901950.1 type IV pilus secretin PilQ Machinery gene
  HB760_RS15025 (HB760_14950) pilP 34098..34613 (-) 516 WP_255899049.1 pilus assembly protein PilP Machinery gene
  HB760_RS15030 (HB760_14955) pilO 34603..35196 (-) 594 WP_255899047.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB760_RS15035 (HB760_14960) pilN 35189..35764 (-) 576 WP_256113731.1 PilN domain-containing protein Machinery gene
  HB760_RS15040 (HB760_14965) pilM 35748..36764 (-) 1017 WP_255899045.1 type IV pilus biogenesis protein PilM Machinery gene
  HB760_RS15045 (HB760_14970) - 36943..39510 (+) 2568 WP_255899043.1 penicillin-binding protein 1A -
  HB760_RS15050 (HB760_14975) oxyR 39589..40497 (-) 909 WP_255899040.1 DNA-binding transcriptional regulator OxyR -
  HB760_RS15055 (HB760_14980) - 40672..41400 (+) 729 WP_255899037.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37876.29 Da        Isoelectric Point: 6.3141

>NTDB_id=433032 HB760_RS15040 WP_255899045.1 35748..36764(-) (pilM) [Vibrio campbellii strain LJC015]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGSRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGATTPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=433032 HB760_RS15040 WP_255899045.1 35748..36764(-) (pilM) [Vibrio campbellii strain LJC015]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAATCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAAAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTAGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACGATGTTCACCGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTTCTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTACAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTCTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

98.225

100

0.982
  pilM Vibrio cholerae strain A1552

49.415

100

0.5